Structure and Assembly of the Nuclear Pore Complex

Hampoelz, Bernhard; Andrés-Pons, Amparo; Kastritis, Panagiotis L.; Beck, Martin

doi:10.1146/annurev-biophys-052118-115308

Cited by 238 publications

(269 citation statements)

References 145 publications

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“…Nucleocytoplasmic shuttling of all substances needed for transcription and translation and numerous other cellular processes is mediated by the nuclear pore complex (NPC) [1][2][3] . NPC associates with and stabilizes a highly curved section of the NEnamely the fusion between the inner (INM) and outer nuclear membranes (ONM) 4,5 .…”

Section: Introductionmentioning

confidence: 99%

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Molecular architecture of the luminal ring of theXenopus laevisnuclear pore complex

Zhang

Zeng

et al. 2020

Preprint

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Nuclear pore complex (NPC) mediates the flow of substances between the nucleus and cytoplasm in eukaryotic cells. Here we report the cryo-electron tomography (cryo-ET) structure of the luminal ring (LR) of the NPC from Xenopus laevis oocyte. The observed key structural features of the LR are independently confirmed by single-particle cryo-electron microscopy (cryo-EM) analysis. The LR comprises eight butterfly-shaped subunits, each containing two symmetric wings. Each wing consists of four elongated, tubular protomers. Within the LR subunit, the eight protomers form a Finger domain, which directly contacts the fusion between the inner and outer nuclear membranes, and a Grid domain, which serves as a rigid base for the Finger domain. Two neighbouring LR subunits interact with each other through the lateral edges of their wings to constitute a Bumper domain, which displays two major conformations and appears to cushion neighbouring NPCs. Our study reveals previously unknown features of the LR and potentially explains the elastic property of the NPC.

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Section: Introductionmentioning

confidence: 99%

“…NPC has an eight-fold symmetry along the nucleocytoplasmic axis and a pseudo two-fold rotational symmetry in the plane of the NE. The scaffold of an NPC is proposed to comprise a cytoplasmic ring (CR), an inner ring (IR) and a nuclear ring (NR) 5 . Cytoplasmic filaments and nuclear basket are attached to the CR and NR, respectively 5,10 .…”

Section: Introductionmentioning

confidence: 99%

Molecular architecture of the luminal ring of theXenopus laevisnuclear pore complex

Zhang

Zeng

et al. 2020

Preprint

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“…To investigate whether NPCs change in Box 1. The structure and function of the nuclear pore complex at a glance The NPC structure, recently reviewed in [15], is made of~30 different proteins, called Nucleoporins or Nups, that are present in multiple copies (Fig. 1).…”

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confidence: 99%

Poor old pores—The challenge of making and maintaining nuclear pore complexes in aging

2020

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The nuclear pore complex (NPC) is the sole gateway to the nuclear interior, and its function is essential to all eukaryotic life. Controlling the functionality of NPCs is a tremendous challenge for cells. Firstly, NPCs are large structures, and their complex assembly does occasionally go awry. Secondly, once assembled, some components of the NPC persist for an extremely long time and, as a result, are susceptible to accumulate damage. Lastly, a significant proportion of the NPC is composed of intrinsically disordered proteins that are prone to aggregation. In this review, we summarize how the quality of NPCs is guarded in young cells and discuss the current knowledge on the fate of NPCs during normal aging in different tissues and organisms. We discuss the extent to which current data supports a hypothesis that NPCs are poorly maintained during aging of nondividing cells, while in dividing cells the main challenge is related to the assembly of new NPCs. Our survey of current knowledge points toward NPC quality control as an important node in aging of both dividing and nondividing cells. Here, the loss of protein homeostasis during aging is central and the NPC appears to both be impacted by, and to drive, this process.

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“…The NPC has a cylindrical appearance and resides at the site of fusion between the inner and outer nuclear membranes. Along the cylindrical axis from the cytoplasmic side, an NPC consists of cytoplasmic filaments (CF), a cytoplasmic ring (CR), an inner ring (IR), a nuclear ring (NR) and a nuclear basket (NB) 3,5,20 . The ring scaffolds associate with one another and with the membrane.…”

Section: Introductionmentioning

confidence: 99%

Structure of the Cytoplasmic Ring of theXenopus laevisNuclear Pore Complex

Huang

Zhang

Zhu

et al. 2020

Preprint

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Huang et al 2 Nuclear pore complex (NPC) exhibits structural plasticity and has only been characterized at local resolutions of up to 15 Å for the cytoplasmic ring (CR). Here we present a single-particle cryo-electron microscopy (cryo-EM) structure of the CR from Xenopus laevis NPC at average resolutions of 5.5-7.9 Å, with local resolutions reaching 4.5 Å. Improved resolutions allow identification and placement of secondary structural elements in the majority of the CR components. The two Y complexes in each CR subunit interact with each other and associate with those from flanking subunits, forming a circular scaffold. Within each CR subunit, the Nup358-containing region wraps around the stems of both Y complexes, likely stabilizing the scaffold. Nup205 connects the short arms of the two Y complexes and associates with the stem of a neighbouring Y complex. The Nup214-containing region uses an extended coiled-coil to link Nup85 of the two Y complexes and protrudes into the axial pore of the NPC. These previously uncharacterized structural features reveal insights into NPC assembly.

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Structure and Assembly of the Nuclear Pore Complex

Cited by 238 publications

References 145 publications

Molecular architecture of the luminal ring of theXenopus laevisnuclear pore complex

Molecular architecture of the luminal ring of theXenopus laevisnuclear pore complex

Poor old pores—The challenge of making and maintaining nuclear pore complexes in aging

Structure of the Cytoplasmic Ring of theXenopus laevisNuclear Pore Complex

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