Structure and biosensor characteristics of complex between glucose oxidase and plasma-polymerized nanothin film

Deposition of chemically reactive and repellent sites on biosensor chips for reduced non-specific binding

“…The use of gas-phase processes can overcome such drawbacks and represents a class of relatively straightforward methods to functionalize the plastic surface. [5][6][7][8][9] A one step process of surface functionalization, by plasma enhanced chemical vapour deposition (PECVD), is more appealing than the multistep, [10][11][12][13] wet chemical process. In…”

Amperometric Biosensor Based on Glucose Dehydrogenase and Plasma-polymerized Thin Films

“…10 nm from the crystallographic data, 23 the immobilized GDH array on PPF surface was probably covered with the 9-nm-and 18-nm-thickness PPF-coatings. 24 From Figs. 2b and 2c, we see that the response times (to reach 90% of the steady-state current) of the device with 9 nmthickness PPF-overcoating were 25 s, whereas those with 18 nmthickness was 105 s. The difference is because the 18 nmthickness PPF blocked the access of the substrate (glucose) more strongly than the 9 nm-thickness PPF.…”

“…Our group has already reported that the other enzyme (GOx) treated by plasma retained its activity. 24 Therefore, this fact can diverse the process for dry-chemical base biosensors and biochips. Figure 5 shows the operational stability under continuous polarization at +0.6 V in the presence of 9.6 mM glucose.…”

Optimization of Carbon Nanotube Layer Formation on Plasma-Polymerized Thin Film for Enzyme Biosensor

“…The immobilized GOx molecules at the surface are in the form of a compact ellipsoid with approximate dimensions of 12.2 × 8.3 nm 2 (measured by AFM). (21,22) Therefore, the redox center exists at a smaller pocket in the larger (10 nm) three-dimensional structure of GOx. The dimension of a single-walled CNT (1.2-2 nm) is smaller than that of GOx; therefore, access to the reaction center is possible.…”

“…Using a different approach, we have proposed an amperometric biosensor based on a plasma-polymerized thin film (PPF), in which the PPF is achieved by glow discharge or plasma in the vapor phase. (19)(20)(21)(22) For the PPF, acetonitrile, a monomer, is suitable for the interfacial design between the biological component (enzyme) and the electrode, because of the highly branched and crosslinked aliphatic hydrocarbon backbone chains that contain nitrogen atoms in the form of primary amine groups.…”