Structure and composition of ferritin cores from pea seed (Pisum sativum)

Wade, Vanessa J.; Treffry, Amyra; Laulhère, Jean-Pierre; Bauminger, E. R.; Cleton, Maud I.; Mann, Stephen; Briat, Jean-François; Harrison, Pauline M.

doi:10.1016/0167-4838(93)90201-2

Cited by 94 publications

(75 citation statements)

References 36 publications

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“…Details of the constraints applied during these fits are given in the Electronic Supplementary Material (Online Resource 2), the corresponding results are listed in Table 1 and Table 2 (Table 1, Table 2). The relatively low average hyperfine magnetic field is similar to values found for the hydrous ferric oxide core of bacterial and plant ferritins which are in the range of 41-45 T (St. Pierre et al 1986, Wade et al 1993, Hartnett et al 2012. The spectra measured at T = 5 K (Fig 1 a,d) reflects a considerable collapse and broadening of this main magnetic sextet component suggesting that the corresponding magnetic phase undergoes a transition to the paramagnetic state at around ∼ 5 K. The excessive broadening of the peaks of the sextet as observed at 5 K may refer to the presence of magnetic relaxation phenomena appearing due to the nanosized nature of the associated magnetic particles, as well as to a distribution in the hyperfine magnetic field occurring due to the presence of a multitude of iron microenvironments with slightly different characteristic magnetic transition temperatures.…”

Section: Resultssupporting

confidence: 55%

“…It is known that plant and bacterial ferritins have low magnetic ordering temperature (< 4 K) and exhibit a significantly lower hyperfine magnetic field (40-44 T) than iron-rich mammalian ferritins (48-50 T) due to their amorphous structure and high phosphate content (St. Pierre et al 1986, Chasteen et al 1999, Wade et al 1993, Cornell & Schwertmann 2003, Hartnett et al 2012. Therefore, it is plausible to regard the well-developed magnetic sextet component Fe B1 in our spectra (at T= 2 K, Fig.…”

Section: Resultsmentioning

confidence: 99%

“…duckweed, soybean, stocks, pea, rice were investigated (Goodman and Dekock 1982a,b, Kilcoyne et al 2000, Wade et al 1993. The main Fe component was identified as ferritin or γ-FeOOH precipitated on the surface of the roots.…”

Section: Abstract Introductionmentioning

confidence: 99%

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Revisiting the iron pools in cucumber roots: identification and localization

Kovács

Pěchoušek

Machala

et al. 2016

Planta

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Main ConclusionFe deficiency responses in Strategy I causes a shift from the formation of partially removable hydrous ferric oxide on the root surface to the accumulation of Fe-citrate in the xylem. 2 AbstractIron may accumulate in various chemical forms during its uptake and assimilation in roots.The permanent and transient Fe microenvironments formed during these processes in cucumber which takes up Fe in a reduction based process (Strategy I), have been investigated.

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Section: Resultssupporting

confidence: 55%

Section: Resultsmentioning

confidence: 99%

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Revisiting the iron pools in cucumber roots: identification and localization

Kovács

Pěchoušek

Machala

et al. 2016

Planta

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“…In plants, phosphate is part of the mineral core of ferritins, and the ratio is about 1 phosphate for 3 iron atoms (34). An attractive hypothesis would be that ferritins are necessary to regulate phosphate homeostasis in plastids, since these proteins store phosphate as well as iron.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis Ferritin 1 (AtFer1) Gene Regulation by the Phosphate Starvation Response 1 (AtPHR1) Transcription Factor Reveals a Direct Molecular Link between Iron and Phosphate Homeostasis

Bournier

Tissot

Mari

et al. 2013

Journal of Biological Chemistry

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148

131

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Background: Physiological evidences have linked phosphate and iron nutrition in plants. Results: Both PHR1 and PHL1 interact with AtFer1 promoter region and regulate its expression in an iron-independent manner. Conclusion: A molecular link exists between the control of iron and of phosphate homeostasis. Significance: PHR1 and PHL1 play a critical role in the regulation of both phosphate and iron homeostasis.

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“…Accordingly, it is synthesized as an N-terminally extended precursor that is post-translationally targeted to the plastid. Although ferritin is encoded by a multigene family in plants (four FER genes in Arabidopsis; Petit et al 2001a), the subunits are all of one type with a ferroxidase active site and characteristics of both the H and the L chain (Wade et al 1993). Plant ferritins also have a distinctive extension peptide (EP) not found in mammalian homologs.…”

mentioning

confidence: 99%

FER1 and FER2 Encoding Two Ferritin Complexes in Chlamydomonas reinhardtii Chloroplasts Are Regulated by Iron

Long¹,

Sommer²,

Allen

et al. 2008

Genetics

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Two unlinked genes FER1 and FER2 encoding ferritin subunits were identified in the Chlamydomonas genome. An improved FER2 gene model, built on the basis of manual sequencing and incorporation of unplaced reads, indicated 49% identity between the ferritin subunits. Both FER1 and FER2 transcripts are increased in abundance as iron nutrition is decreased but the pattern for each gene is distinct. Using subunitspecific antibodies, we monitored expression at the protein level. In response to low iron, ferritin1 subunits and the ferritin1 complex are increased in parallel to the increase in FER1 mRNA. Nevertheless, the iron content of the ferritin1 complex is decreased. This suggests that increased expression results in increased capacity for iron binding in the chloroplast of iron-limited cells, which supports a role for ferritin1 as an iron buffer. On the other hand, ferritin2 abundance is decreased in iron-deprived cells, indicative of the operation of iron-nutrition-responsive regulation at the translational or post-translational level for FER2. Both ferritin subunits are plastid localized but ferritin1 is quantitatively recovered in soluble extracts of cells while ferritin2 is found in the particulate fraction. Partial purification of the ferritin1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of ferritin1 to ferritin2 is 70:1 in iron-replete cells, suggestive of a more dominant role of ferritin1 in iron homeostasis. The Volvox genome contains orthologs of each FER gene, indicating that the duplication of FER genes and potential diversification of function occurred prior to the divergence of species in the Volvocales.A LTHOUGH iron is abundant on earth, its bioavailability is limited in the aerobic world because of the insolubility of ferric salts, and iron can be a limiting nutrient for most forms of life. A third of the agricultural land and the same fraction of the ocean are considered iron deficient (reviewed by Boyd et al. 2007). Therefore, iron nutrition is a key component of global productivity. Organisms have evolved multiple and varied pathways for assimilating iron in its various chemical forms and at a range of concentrations in the nutrient environment (Staiger 2002; reviewed in Curie and Briat 2003;Hentze et al. 2004). Even though iron can be toxic to cells as a consequence of its propensity for participating in redox chemistry, cells do not generally excrete iron because it is a limiting nutrient (Liochev and Fridovich 1999). Instead, cells tend to store intracellular iron in a less reactive and hence nontoxic form. Because of the importance of iron for life, these pathways of uptake and storage are subject to layers of homeostatic regulation.We have developed Chlamydomonas as a model organism for understanding trace metal nutrition in plants, especially in the context of chloroplast function and photosynthesis (Merchant et al. 2006). As a microorganism, Chlamydomonas lends itself to nutritional studies because of the ease wit...

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Structure and composition of ferritin cores from pea seed (Pisum sativum)

Cited by 94 publications

References 36 publications

Revisiting the iron pools in cucumber roots: identification and localization

Revisiting the iron pools in cucumber roots: identification and localization

Arabidopsis Ferritin 1 (AtFer1) Gene Regulation by the Phosphate Starvation Response 1 (AtPHR1) Transcription Factor Reveals a Direct Molecular Link between Iron and Phosphate Homeostasis

FER1 and FER2 Encoding Two Ferritin Complexes in Chlamydomonas reinhardtii Chloroplasts Are Regulated by Iron

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