Structure and composition of microcalcifications in benign and malignant lesions of the breast: Study by light microscopy, transmission and scanning electron microscopy, microprobe analysis, and X-ray diffraction

Frappart, Lucien; Boudeulle, M.; Boumendil, Jacqueline; Lin, Hu; Martinon, I.; Palayer, C.; Mallet-Guy, Y; Raudrant, D.; Brémond, A.; Rochet, Y; Féroldi, J

doi:10.1016/s0046-8177(84)80150-1

Cited by 121 publications

(99 citation statements)

References 10 publications

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“…In a preliminary step, we validated our findings by designing a phantom using simulants mimicking the main chemical composition of 7 , and are therefore expected to induce stronger refraction to X-rays passing through them. This behaviour affects the small-angle scattering signal recorded with GI 18 and, as a consequence, it becomes detectable.…”

Section: Resultsmentioning

confidence: 68%

“…The whole samples are embedded in paraffin blocks and sliced with 5 mm thickness to form a three-dimensional data set. All slices are then stained (standard haematoxylin and eosin stain) and examined by microscopy with both unpolarized and polarized light to identify the birefringent and non-birefrigent microcalcifications 7 . The types and locations of the found microcalcifications are marked and the histopathological findings are compared with the experimental imaging results.…”

Section: Methodsmentioning

confidence: 99%

“…In the clinical routine, areas with suspicious microcalcifications within a breast are further investigated by stereotactic vacuum biopsies, followed by the histopathological workup. Frappart et al 6,7 have introduced the existence of two types of microcalcifications of different chemical composition as well as their correlation with benign and malignant breast lesions [6][7][8] . Type I microcalcifications consist of calcium oxalate dihydrate while type II microcalcifications are composed of calcium phosphates, mainly calcium hydroxyapatite 9 .…”

mentioning

confidence: 99%

“…Raman spectroscopy is chemical specific, and therefore well suited to determine different types of microcalcifications, but it works only on very thin, sliced tissue samples, obtained invasively with a biopsy, owing to the very limited penetration power of the probing radiation. In histopathology, using haematoxylin and eosin staining, the two types of microcalcifications show different refractive properties (type I is found to be birefringent, while type II is non-birefringent 6,7,9 ) when polarized light is used thus allowing their discrimination.…”

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confidence: 99%

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Non-invasive classification of microcalcifications with phase-contrast X-ray mammography

et al. 2014

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Microcalcifications can be indicative in the diagnosis of early breast cancer. Here we report a non-invasive diagnostic method that may potentially distinguish between different types of microcalcifications using X-ray phase-contrast imaging. Our approach exploits the complementary nature of the absorption and small-angle scattering signals of microcalcifications, obtained simultaneously with an X-ray grating interferometer on a conventional X-ray tube. We demonstrate that the new approach has 100% sensitivity and specificity when applied to phantom data, and we provide evidence of the solidity of the technique by showing its discrimination power when applied to fixed biopsies, to non-fixed tissue specimens and to fresh, whole-breast samples. The proposed method might be further developed to improve early breast cancer diagnosis and has the potential to increase the diagnostic accuracy and reduce the number of uncomfortable breast biopsies, or, in case of widespread microcalcifications, to select the biopsy site before intervention.

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Section: Resultsmentioning

confidence: 68%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Non-invasive classification of microcalcifications with phase-contrast X-ray mammography

et al. 2014

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“…The characteristics of the mineral deposits isolated from tissues may help diagnose the cause of the disease and determine the developmental stage of the focus. Notably, some typical mineral deposits have become an auxiliary sign for malignant tumor diagnosis (Frappart et al, 1984;Gonzalez et al, 1991;Fandos -Morera et al, 1988;Lanyi, 1985;Sorbe and Frankendal, 1982). However, the significance of the mineral deposits in pathological tissues and their potential use as indicators for the stage of disease have not yet been fully explored or understood, especially not from the viewpoint of mineralogy.…”

Section: Introductionmentioning

confidence: 99%

A study on psammoma body mineralization in meningiomas

Wang

Yang

et al. 2011

Journal of Mineralogical and Petrological Sciences

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Psammoma bodies (PBs) in meningioma patients often contain mineral deposits. In this paper, the mineralogical characteristics of PBs in the mineralized focus in meningioma patients were investigated by using scanning electron microscopy (SEM), environmental scanning electron microscopy (ESEM), high -resolution transmission electron microscopy (HRTEM), energy dispersive X -ray analysis (EDAX), and electron probe microanalysis (EPMA). We found that PBs had a concentric layered structure and the size ranged from several to tens of micrometers. Although no obvious trend in the variation of Ca/P ratios was observed from the center to the outer edge of the PBs, the Ca/P ratios were much lower in the early stage of PBs formation. The concentric layered structure of the PBs might be formed by a spiral arrangement of mineralized collagen fibers. Studies on PBs mineralization may provide some supporting information in understanding the origin and development of meningiomas.

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Multiple Pathways for Pathological Calcification in the Human Body

Vidavsky

Kunitake

Estroff

2020

Adv Healthcare Materials

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Biomineralization of skeletal components (e.g., bone and teeth) is generally accepted to occur under strict cellular regulation, leading to mineral–organic composites with hierarchical structures and properties optimized for their designated function. Such cellular regulation includes promoting mineralization at desired sites as well as inhibiting mineralization in soft tissues and other undesirable locations. In contrast, pathological mineralization, with potentially harmful health effects, can occur as a result of tissue or metabolic abnormalities, disease, or implantation of certain biomaterials. This progress report defines mineralization pathway components and identifies the commonalities (and differences) between physiological (e.g., bone remodeling) and pathological calcification formation pathways, based, in part, upon the extent of cellular control within the system. These concepts are discussed in representative examples of calcium phosphate‐based pathological mineralization in cancer (breast, thyroid, ovarian, and meningioma) and in cardiovascular disease. In‐depth mechanistic understanding of pathological mineralization requires utilizing state‐of‐the‐art materials science imaging and characterization techniques, focusing not only on the final deposits, but also on the earlier stages of crystal nucleation, growth, and aggregation. Such mechanistic understanding will further enable the use of pathological calcifications in diagnosis and prognosis, as well as possibly provide insights into preventative treatments for detrimental mineralization in disease.

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Structure and composition of microcalcifications in benign and malignant lesions of the breast: Study by light microscopy, transmission and scanning electron microscopy, microprobe analysis, and X-ray diffraction

Cited by 121 publications

References 10 publications

Non-invasive classification of microcalcifications with phase-contrast X-ray mammography

Non-invasive classification of microcalcifications with phase-contrast X-ray mammography

A study on psammoma body mineralization in meningiomas

Multiple Pathways for Pathological Calcification in the Human Body

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