Structure and function of vascular endothelial growth factor and its receptor system

Park, Seong Ah; Jeong, Mi Suk; Ha, Ki‐Tae; Jang, Se Bok

doi:10.5483/bmbrep.2018.51.2.233

Cited by 76 publications

(63 citation statements)

References 60 publications

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“…Interestingly, HKMECs cocultured in VEGF-supplemented media (blue bars) showed less upregulation on average of the aforementioned genes except for ANG2, as compared to HKMECs cocultured in VEGF-free media (green bars). These data suggest that in coculture, HPTECs secrete not only VEGF but also other soluble factors supporting HKMECs to maintain key kidney endothelial features via paracrine signaling (22,43,56). In contrast to the genes discussed in Fig.…”

Section: Hptecs Support Expression Of Key Hkmec Organ-specific and Acmentioning

confidence: 83%

Open microfluidic coculture reveals paracrine signaling from human kidney epithelial cells promotes kidney specificity of endothelial cells

Zhang

Lih

Nagao

et al. 2020

Preprint

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AbstractEndothelial cells (ECs) from different human organs possess organ-specific characteristics that support specific tissue regeneration and organ development. EC specificity are identified by both intrinsic and extrinsic cues, among which, parenchyma and organ-specific microenvironment are critical contributors. These extrinsic cues are, however, largely lost during ex vivo cultures. Outstanding challenges remain to understand and re-establish EC organ-specificity for in vitro studies to recapitulate human organ-specific physiology. Here, we designed an open microfluidic platform to study the role of human kidney tubular epithelial cells in supporting EC specificity. The platform consists of two independent cell culture regions segregated with a half wall; culture media is added to connect the two culture regions at a desired timepoint, and signaling molecules can travel across the half wall (paracrine signaling). Specifically, we report that in the microscale coculture device, primary human kidney proximal tubular epithelial cells (HPTECs) rescued primary human kidney peritubular microvascular EC (HKMEC) monolayer integrity and fenestra formation, and HPTECs upregulated key HKMEC kidney-specific genes (HNF1B, AJAP1, KCNJ16) and endothelial activation genes (VCAM1, MMP7, MMP10) in coculture. Co-culturing with HPTECs also promoted kidney-specific genotype expression in human umbilical vein ECs (HUVECs), and human pluripotent stem cell-derived ECs (hPSC-ECs). In comparison to the culture in HPTEC conditioned media, co-culture of ECs with HPTECs showed increased upregulation of kidney specific genes, suggesting potential bidirectional paracrine signaling. Importantly, our device is compatible with standard pipettes, incubators, and imaging readouts, and could also be easily adapted to study cell signaling between other rare or sensitive cells.

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Section: Hptecs Support Expression Of Key Hkmec Organ-specific and Acmentioning

confidence: 83%

Open microfluidic coculture reveals paracrine signaling from human kidney epithelial cells promotes kidney specificity of endothelial cells

Zhang

Lih

Nagao

et al. 2020

Preprint

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“…Late-stage of breast cancer often accompanies with clinical metastasis to the lung, liver, brain, and bone [22]. During bone metastasis, breast cancer cells have to undergo an invasion-transfer cascade to convert themselves to metastatic cells [23,24]. Cancer cells often concentrate on the endosteal after reaching the bone site and then break the delicate balance between osteoblasts and osteoclasts in the bone microenvironment.…”

Section: Discussionmentioning

confidence: 99%

Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Suppresses Migration of Bone‐Metastatic Breast Cancer Cells via OPN‐FAK/ERK/NF‐κB Axis

Fang

Yan

Yang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Hedyotis diffusa plus Scutellaria barbata is a couplet of medicinal that has been commonly used to treat inflammation-related diseases and various types of tumors. However, the effect of this couplet on tumor cell migration has not been elucidated. With the aid of MCF-7-BOM, a bone-metastatic subline of ER + breast cancer MCF-7, we showed that ethyl acetate fraction extracted at an equal weight ratio of Hedyotis diffusa plus Scutellaria barbata (EA11) inhibited cell migration of MCF-7-BOM in a concentration-dependent manner. To define the underlying molecular mechanism, we revealed that EA11 reduced the expression of osteopontin (OPN) and interfered with the FAK/ERK/NF-κB signaling pathways, which are both critical for breast cancer bone metastasis. This study strongly suggested EA11 may represent a potential therapeutic agent against bone metastasis of breast cancer.

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“…The VEGF family contains VEGF‐A, VEGF‐B, VEGF‐C, VEGF‐D and placental growth factor . It is well believed that VEGF‐A plays a central role in abundant angiogenic processes, which consist of EC migration, proliferation and tube formation …”

Section: Vascular Endothelial Growth Factormentioning

confidence: 99%

“…64 It is well believed that VEGF-A plays a central role in abundant angiogenic processes, which consist of EC migration, proliferation and tube formation. 67 The receptors of VEGF-A are the VEGFR-1 (fmsrelated tyrosine kinase1, FLT1) and VEGFR-2 (kinase insert domain receptor, KDR or Flk1). Both are highaffinity receptor tyrosine kinases that are largely expressed on the surface of EC.…”

Section: Vascular Endothelial Growth Factormentioning

confidence: 99%

Inflammation and angiogenesis in the corpus luteum

Wang

et al. 2019

J of Obstet and Gynaecol

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Angiogenesis is a very important process that helps establish and maintain the normal structure and function of the corpus luteum (CL). Early luteal development can be considered a kind of physiological injury with an inflammatory response; therefore, the inflammatory response may play an important role in the luteal angiogenesis. The inflammatory response is companied by activated leukocytes and their mediators. For luteal tissue, numerous activated leukocytes such as macrophages, neutrophils and eosinophils are present in the early luteal phase and are widely involved in neovascularization. The objective of this review is to describe the role of the inflammatory factors in the angiogenesis and to discuss their mechanism. Knowledge of action and mechanism of these inflammatory factors on angiogenic activity will be beneficial for the understanding of luteal function.

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Structure and function of vascular endothelial growth factor and its receptor system

Cited by 76 publications

References 60 publications

Open microfluidic coculture reveals paracrine signaling from human kidney epithelial cells promotes kidney specificity of endothelial cells

Open microfluidic coculture reveals paracrine signaling from human kidney epithelial cells promotes kidney specificity of endothelial cells

Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Suppresses Migration of Bone‐Metastatic Breast Cancer Cells via OPN‐FAK/ERK/NF‐κB Axis

Inflammation and angiogenesis in the corpus luteum

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