Structure and functional capacity of a benzene-mineralizing, nitrate-reducing microbial community

Abstract

This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Anaerobe isolation from denitrifying benzene-degrading enrichment culture and their capacity to mineralize benzene

“…Pseudomonadaceae (0 -21%)), and unknown Rhizobiaceae (0 -0.2%), Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium (0 -0.2%) (Family: Rhizobiaceae (< 1%)) were detectable at the indicated range of relative abundances throughout the time-course of the previous study done with the original inoculum (Eziuzor et al, 2022). Thus, the corresponding phylotypes identified in acetate-grown consortia showed a snapshot of possible isolates under the classical isolation procedure employed here.…”

“…The copyright holder for this preprint this version posted January 7, 2023. ; https://doi.org/10.1101/2023.01.07.522375 doi: bioRxiv preprint fixing symbionts mostly found naturally in legumes (Mousavi et al, 2014) and have been identified in anaerobic microbial communities degrading acetate under sulfate-reducing conditions (Bin Hudari et al, 2020), and benzene under nitrate-reducing conditions (Eziuzor et al, 2022), which is the original inoculum for the isolation attempt. ANPR was also found to be the dominant phenanthrene degrader aerobically in gradient anthropized soils (Lemmel et al, 2019).…”

“…Anaerobes are known to have a long lag phase and, in this case, would require more time to acclimatize in the medium. A lag phase of around 70 days was observed in the original benzene-mineralizing, nitrate-reducing microcosms (Eziuzor et al, 2022). Considering the long lag phases observed in anaerobic cultivation and the inherent acclimatization of the cells in their new, unnatural media, it is expected that the isolates will take even a longer time to acclimatize, proliferate and metabolically degrade the stable benzene compound only if favoured anaerobically.…”

“…All bacterial phylotypes identified in the acetate-grown consortia as uncultured Anaerolineaceae (0 – 4.4%) (Family: Anaerolineaceae (1 – 9%)); Simplicispira (0 – 3.2%) (Family: Burkholderiaceae (2 – 11%)); Nocardioides (0 – 6.9%) (Family: Nocardioidaceae (0 – 9%)); Pseudomonas (0 – 11.4%) (Family: Pseudomonadaceae (0 – 21%)), and unknown Rhizobiaceae (0 – 0.2%), Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium (0 – 0.2%) (Family: Rhizobiaceae (< 1%)) were detectable at the indicated range of relative abundances throughout the time-course of the previous study done with the original inoculum (Eziuzor et al, 2022). Thus, the corresponding phylotypes identified in acetate-grown consortia showed a snapshot of possible isolates under the classical isolation procedure employed here.…”

“…A denitrifying benzene-mineralizing culture enriched from a former benzene-contaminated aquifer near Zeitz in Germany in on-site columns containing coarse sands; originally enriched as a sulfate-reducing culture but later was adapted to the denitrifying condition and maintained for several years in the laboratory (Vogt et al, 2007; Keller et al, 2018; Eziuzor et al, 2022). The enrichment culture is made up of both liquid-phase and solid phases comprising coarse sands.…”