Structure and Hydration of Highly-Branched, Monodisperse Phytoglycogen Nanoparticles

Nickels, Jonathan D.; Atkinson, John; Papp-Szabó, Erzsébet; Stanley, Christopher B.; Diallo, Souleymane; Perticaroli, Stefania; Baylis, Benjamin; Mahon, Perry T.; Ehlers, Georg; Katsaras, John; Dutcher, John

doi:10.1021/acs.biomac.5b01393

Cited by 83 publications

(115 citation statements)

References 61 publications

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“…The inset shows an isolated nanoparticle in water, with a line profile through its center, indicating its flattened nature (horizontal axis is 90 nm and range of the vertical axis is 12.5 nm). Adapted with permission . Copyright 2016, American Chemical Society.…”

Section: Structure and Physicochemical Properties Of Glycogen Nanoparmentioning

confidence: 99%

“…Glycogen can be readily obtained commercially from oyster, slipper limpet, bovine and rabbit liver, and sweet corn (termed phytoglycogen) . This availability makes glycogen well positioned for use in a material‐based application.…”

Section: Structure and Physicochemical Properties Of Glycogen Nanoparmentioning

confidence: 99%

“…This interaction can lead to interesting solvation phenomena that have use, for example, in moisturizers, rheological modifiers, and emulsifiers . Like other polysaccharides, glycogen has a very well hydrated structure . From a biological perspective, glycogen constitutes a high proportion of wet mass of various organs.…”

Section: Structure and Physicochemical Properties Of Glycogen Nanoparmentioning

confidence: 99%

“…In contrast to the comparable hydration water structure displayed by the two linear polysaccharides, that of phytoglycogen was significantly different, suggesting that the high degree of branching in phytoglycogen allows for much more well‐ordered water clusters. Nickels et al used neutron scattering to interpret the hydration of small dense phytoglycogen nanoparticles, achieved through determination of the neutron scattering length density (NSLD) match point of PG nanoparticles (i.e., the ratio of H 2 O to D 2 O where the scattering from the molecule equals that of the solvent), which allowed for the calculation of the molecular volume per glucose monomer. This in turn was used in calculations for the approximate number of water molecules associated with each glucose monomer (obtained through a best fit of the NSLD at 100% D 2 O), which suggested that the particles contained ≈23 water molecules per glucose monomer.…”

Section: Structure and Physicochemical Properties Of Glycogen Nanoparmentioning

confidence: 99%

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Glycogen as a Building Block for Advanced Biological Materials

2019

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complex drug delivery systems that are challenging to scale up and therefore have limited clinical and commercial translation. Although numerous nanoparticles have been widely developed and used for various applications, including biomedical applications, [2] there is a growing interest in nontoxic and degradable alternatives to first-generation synthetic nanomaterials. Ideally the synthesis of such nanoparticles needs to be cost effective, simple, green, reproducible, scalable, and the constitutive building blocks of the nanoparticles should be nontoxic, functional, biodegradable, and available on a large scale. Naturally occurring nanoparticles could be such a valid alternative. These nanoparticles include intracellular structures, such as magnetosomes [3] and glycogen, and extracellular assemblies such as lipoproteins and viruses. [3] Among these, glycogen is the most abundant and versatile biological nanoparticle, which fulfils the requirements for the fabrication of nanostructured biomaterials. Glycogen is nature's prime nanoparticle that exists in most organisms, from bacteria and archaea to humans, [4,5] as a vital component of the cellular energy machinery. It is a highly branched polysaccharide comprising repeating units of glucose connected by linear α-d-(1,4) glycosidic linkages with α-d-(1,6) branching, structured into roughly spherical nanoparticles that have a high water solubility and molecular weight.The use of polysaccharides as components for advanced materials has been an attractive concept for decades. [6,7] A key motive is that polysaccharides, as a natural biomaterial, are endowed with a level of biodegradability and biocompatibility. In addition, they can be easily modified chemically or biochemically to produce functional derivatives, which are important for various therapeutic applications. [8] The structures of polysaccharides vary considerably but span from linear to highly branched polymers with molecular weights ranging from thousands to millions, composed of mono-or disaccharides, or short-chain oligomers bound together by glycosidic linkages. [9] There is a rich history of research on the use of polysaccharides in a range of therapeutic applications including: i) soft tissue engineering, [10,11] where the materials can be designed to mimic the mechanical properties of the extracellular matrix in the tissue; ii) as drug, protein, or gene delivery systems, [7,12,13] where polysaccharides have a large number of reactive groups [14] that allow for tunable properties [12] such as pH-responsiveness; [15] and iii) as nanoprobes for cellular Biological nanoparticles found in living systems possess distinct molecular architectures and diverse functions. Glycogen is a unique biological polysaccharide nanoparticle fabricated by nature through a bottom-up approach. The biocatalytic synthesis of glycogen has evolved over time to form a nanometer-sized dendrimer-like structure (20-150 nm) with a highly branched surface and a dense core. This makes glycogen markedly different from other natur...

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Section: Structure and Physicochemical Properties Of Glycogen Nanoparmentioning

confidence: 99%

Section: Structure and Physicochemical Properties Of Glycogen Nanoparmentioning

confidence: 99%

Section: Structure and Physicochemical Properties Of Glycogen Nanoparmentioning

confidence: 99%

Section: Structure and Physicochemical Properties Of Glycogen Nanoparmentioning

confidence: 99%

See 2 more Smart Citations

Glycogen as a Building Block for Advanced Biological Materials

2019

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“…The ability to characterize and define nanoparticle interfaces in detail is highly relevant to understanding their function, as interfacial effects govern numerous important properties of nanoparticles such as hydration [14], encapsulation [15], and self-assembly behavior [16] to name but a few. Nevertheless, an accurate description of the nanoparticle interface is seldom incorporated into analysis.…”

Section: Introductionmentioning

confidence: 99%

A novel method for constructing continuous intrinsic surfaces of nanoparticles

Allen

Lorenz

2017

J Mol Model

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In recent years, the field of nanotechnology has become increasingly prevalent in the disciplines of science and engineering due to it’s abundance of application areas. Therefore, the ability to study and characterize these materials is more relevant than ever. Despite the wealth of simulation and modeling studies of nanoparticles reported in the literature, a rigorous description of the interface of such materials is rarely included in analyses which are pivotal to understanding interfacial behavior. We propose a novel method for constructing the continuous intrinsic surface of nanoparticles, which has been applied to a model system consisting of a sodium dodecyl sulfate micelle in the presence of testosterone propionate. We demonstrate the advantages of using our continuous intrinsic surface definition as a means to elucidate the true interfacial structure of the micelle, the interfacial properties of the hydrating water molecules, and the position of the drug (testosterone propionate) within the micelle. Additionally, we discuss the implications of this algorithm for future work in the simulation of nanoparticles. Graphical Abstractᅟ

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Tracking the Endosomal Escape of Nanoparticles in Live Cells Using a Triplex‐Forming Oligonucleotide

Bhangu,

Mummolo,

Fernandes

et al. 2024

Adv Funct Materials

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Nanoparticle‐mediated intracellular delivery of oligonucleotides is a complex phenomenon that depends on the architecture and the intracellular trafficking of the engineered nanoparticles. Unravelling the molecular arrangements of oligonucleotides within the nanoparticles as well as their intracellular behavior are essential for designing effective nucleic acid delivery systems. Herein, a simple and general strategy for probing the endosomal escape of nanoparticles carrying oligonucleotides in live cells is reported. A triplex‐forming oligonucleotide probe is designed to target the transcription factor, kappa‐light‐chain‐enhancer of activated B cells (NF‐κB), in the cytosol of cells and to transduce the binding into a fluorescent Förster resonance energy transfer (FRET) signal. The combined use of the triplex‐forming oligonucleotide probe and super‐resolution microscopy enables the elucidation of the morphology, intracellular localization, and endosomal escape of the oligonucleotide‐loaded nanoparticles on a molecular level and with nanoscale resolution. The co‐delivery of the FRET probe and mRNA in cells via lipid‐ and polymer‐ based nanoparticles allow simultaneous correlation of the endosomal escape properties of nanoparticles and gene expression efficiency.

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Structure and Hydration of Highly-Branched, Monodisperse Phytoglycogen Nanoparticles

Cited by 83 publications

References 61 publications

Glycogen as a Building Block for Advanced Biological Materials

Glycogen as a Building Block for Advanced Biological Materials

A novel method for constructing continuous intrinsic surfaces of nanoparticles

Tracking the Endosomal Escape of Nanoparticles in Live Cells Using a Triplex‐Forming Oligonucleotide

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