Structure and Possible Function of Chloroplast Heat-Shock Proteins and the Effect of Cyclic Heat-Shock on Plant Morphogenesis and Circadian Rhythmicity

Knack, Gaby; Otto, Beate; Ottersbach, Peter; Alexander, R. W.; Liu, Zhonglai; Kloppstech, Klaus

doi:10.1007/978-94-009-0511-5_758

Cited by 7 publications

(7 citation statements)

References 17 publications

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“…In addition, the mRNA levels of several light-inducible genes were considerably enhanced similar to earlier findings in Chlamydomonas where the level of mRNA for LHCP was increased after heat-shock [12]. More importantly, the mRNA levels were found to oscillate in a circadian manner [ 15,16]. Since these effects were observed in complete darkness they were assumed to be independent of the presence of activated phytochrome.…”

Section: Introductionsupporting

confidence: 84%

Temperature treatments of dark-grown pea seedlings cause an accelerated greening in the light at different levels of gene expression

1992

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We have previously shown that heat-shock in the dark evokes photomorphogenesis-like effects and circadian rhythmicity at the level of mRNAs when applied to emerging pea plantlets during several consecutive days [15]. Here we extend these findings by showing that a temperature shift to 10 degrees C above average and a single heat-shock are sufficient for induction of circadian rhythmicity and changes in morphogenesis. The maximum response to a single heat-shock occurs at days 2 to 3 after sowing indicating additional developmental control of the response. An increasing number of heat-shock treatments intensifies the morphogenetic effect. The heat-shocked plantlets have an elevated level of the xanthophyll lutein in the dark. Upon illumination of heat-shocked plantlets accumulation of chloroplast pigments as well as that of individual thylakoid membrane proteins and their corresponding mRNAs occur much faster than in the etiolated controls. This is reflected in an accelerated formation of grana stacks. Therefore, heat-shock seems to evoke a responsiveness of plantlets similar to that obtained earlier by other authors using pre-illumination. The working hypothesis is put forward that induction or synchronization of circadian rhythmicity by either light or heat-shock might be sufficient to explain the observed morphogenetic changes.

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Section: Introductionsupporting

confidence: 84%

Temperature treatments of dark-grown pea seedlings cause an accelerated greening in the light at different levels of gene expression

1992

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“…After the completion of this study, Knack et al. (22) reported the deduced amino acid sequence of a pea LMW HSP cDNA, HSP26-22, encoding a protein identical to PsHSP22.7. They did not identify it as an endomembrane protein.…”

Section: Methodsmentioning

confidence: 99%

Localization of small heat shock proteins to the higher plant endomembrane system.

Helm¹,

LaFayette²,

Nagao³

et al. 1993

Mol. Cell. Biol.

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“…HSP and the corresponding mRNAs are present in some seeds; while especially the mRNAs disappear rapidly during imbibition the proteins remain relatively stable [13]. De novo synthesis during development ofmRNAs for HSP and of the corresponding proteins starts a few days after germination of pea [17]. HSP expression might also be studied in the monocotyledonean leaf which has long been useful as a model for gene expression studies [6,28].…”

Section: Introductionmentioning

confidence: 99%

Expression of heat shock proteins during development of barley

1993

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Barley heat shock proteins have been cloned, characterized by hybrid release translation and sequenced. Clones coding for proteins of 17, 18, 30, 32 and 70 kDa have been obtained. Out of these the 32 and 30 kDa proteins have been characterized as precursors to plastidic proteins of 26 kDa by posttranslational transport and by cDNA sequencing. The coding regions of these two transcribed genes are highly homologous. Accumulation of the plastid HSP as well as of HSP 70 as well as their corresponding mRNAs has been studied in 2- to 6-day old seedlings and in the 7-day old barley leaf. The mRNA for all investigated proteins were only found after a heat shock; the mRNA levels increase towards the tip of the leaf and with development. Furthermore, under the conditions used the mRNAs for all investigated heat shock proteins accumulate in parallel. Unexpectedly, both proteins, HSP 70 and HSP 26, are found by western blotting in the 2-day old control plants in the absence of any inducing heat shock. At later stages of development and in the leaf gradient only immunoreactivity with HSP 70 was observed. In contrast to the levels of their mRNAs the highest levels of HSP 30-26 and 70 have been observed in the basal segments indicating that translational control plays a role during HSP expression. Under severe heat shock a protein of 30 kDa is induced whose identity is not known but which reacts with the antibody to HSP 30-26 and might represent the accumulating precursors of the plastidic proteins.

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Structure and Possible Function of Chloroplast Heat-Shock Proteins and the Effect of Cyclic Heat-Shock on Plant Morphogenesis and Circadian Rhythmicity

Cited by 7 publications

References 17 publications

Temperature treatments of dark-grown pea seedlings cause an accelerated greening in the light at different levels of gene expression

Temperature treatments of dark-grown pea seedlings cause an accelerated greening in the light at different levels of gene expression

Localization of small heat shock proteins to the higher plant endomembrane system.

Expression of heat shock proteins during development of barley

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