Structure and transcription of the genes encoding the B1015 light-harvesting complex beta and alpha subunits and the photosynthetic reaction center L, M, and cytochrome c subunits from Rhodopseudomonas viridis

Abstract

The genes encoding the and a subunits of the B1015 light-harvesting complex (LHC)

Sequence analysis and transcriptional organization of the Rhodopseudomonas viridis cytochrome c2 gene

“…Overall comparison results in the common sequence motif 5'-GA-N5-CG-3' in the -40/-30 region. In R. viridis, the expression of cytochrome c2, reaction center polypeptides, and other proteins involved in the photosynthetic process depends on anaerobic growth conditions (22,24 (45) with those from Rhodobacter capsulatus (1,8), Rhodobacter sphaeroides (23,25), and Rhodospirillum rubrum (9). The common sequence motif in the -40/-30 region is in boldface.…”

“…Recently, the exact 5' and 3' ends of the puf operon transcripts from R. viridis were determined (45). Alignment of the nucleotide sequences upstream from the transcription starts of the puf operon and cycA gene from photosynthetically grown R. viridis cells (Fig.…”

“…The methods for RNA isolation and Northern blot analysis have been described previously (45). RNA isolated from photoheterotrophically grown R. viridis cells was size fractionated on a 1.2% agarose gel, transferred to a nylon membrane by vacuum blotting, and cross-linked to the membrane by UV light.…”

“…Primer extension was performed as described before (6,45). Total RNA (50 ,ug) was hybridized to the 5'-end 32P-labeled 27-mer oligonucleotide 5'-GCCG CGAGAACGAACAGTCCGAACACC-3' (7 x i0' dpm) at 30°C for 12 h. After extension of the hybridized primer by AMV reverse transcriptase (40 U) for 90 min at 50°C, the mixture was phenol extracted and precipitated by ethanol.…”

“…Radioactively labeled hybridization probes were obtained by in vitro transcription of a 1.1-kb Sall-ApaI DNA fragment (fragment C, Fig. 1 (45).…”