2014
DOI: 10.1021/bi500660q
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Structure-Based Engineering of a Minimal Porin Reveals Loop-Independent Channel Closure

Abstract: Porins, like outer membrane protein G (OmpG) of Escherichia coli, are ideal templates among ion channels for protein and chemical engineering because of their robustness and simple architecture. OmpG shows fast transitions between open and closed states, which were attributed to loop 6 (L6). As flickering limits single-channel-based applications, we pruned L6 by either 8 or 12 amino acids. While the open probabilities of both L6 variants resemble that of native OmpG, their gating frequencies were reduced by 63… Show more

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Cited by 27 publications
(52 citation statements)
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“…Nevertheless, the OmpGF still possesses gating properties. In a recent study, residual gating was observed even when all the extracellular loops of OmpG were truncated [53]. This suggests that there are likely structural features other than loop L6 in OmpG that are responsible for gating behavior.…”
Section: Resultsmentioning
confidence: 99%
“…Nevertheless, the OmpGF still possesses gating properties. In a recent study, residual gating was observed even when all the extracellular loops of OmpG were truncated [53]. This suggests that there are likely structural features other than loop L6 in OmpG that are responsible for gating behavior.…”
Section: Resultsmentioning
confidence: 99%
“…These studies suggest that mechanisms other than loop 6 motion may also contribute to OmpG’s spontaneous gating, such as the motion of other loops or even loop-independent gating such as barrel contraction. 37 In addition to pH-dependent and spontaneous gating, OmpG also exhibited a third class of gating, one that was dependent on the applied voltages in the electrophysiological experiment. 7 It is necessary to distinguish these three types of gating behaviors in electrophysiology, as the voltage-gating may have its own mechanism distinct from the other two.…”
Section: Discussionmentioning
confidence: 99%
“…Loop 6 is unlikely to be associated with the voltage-dependent gating of OmpG, as the deletion of loop 6 and removal of all the loops did not abolish voltage-dependent gating. 37 …”
Section: Discussionmentioning
confidence: 99%
“…A non-gating “quietOmpG” was created by stabilizing a loop with a disulphide bond and deleting the bulge of a strand [24]. The gating of OmpG has since been further stabilized by dramatically removing all of the loops [25]. …”
Section: Outer Membrane Protein Designmentioning
confidence: 99%