Structure des parois cellulaires des nocardia. I‐isolement et composition des parois de <i>Nocardia kirovani</i>

Guinand, Micheline; Vacheron, Marie‐Jeanne; Michel, Georges

doi:10.1016/0014-5793(70)80036-9

Cited by 33 publications

(20 citation statements)

References 15 publications

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“…PETIT, ADAM, and others (7,8) reported that Mycobacterium smegmatis had an atypical glycan strand in its cell wall which was identified to be a repeated unit of disaccharide, N-acetylglucosaminyl-~3-1,4-N-glycolylmuramic acid, which was soon demonstrated by AzUMA et al (9) to be also present in three other mycobacteria but not in Corynebacterium fermentans or Streptomyces albus. The same glycan structure was also found in three strains of Nocardia (10,11).…”

supporting

confidence: 73%

“…For the sake of convenience, "glycolyl type" cell wall will be proposed for the name of this kind of bacterial cell wall, if ordinary cell wall could be called "acetyl type," concerning the acyl type of the glycan part of the peptidoglycan. Although it was not decided in this work which amino sugar is acylated with the glycolyl group, the occurrence of N-glycolylmuramic acid has been shown by Azuma et al in the strains of M. phlei ATCC 356, M. tuberculosis BCG, and M. kansasii P21 (9), and by Michel and the co-worker in N. kirovani (10), N. asteroides R399, and N. caviae IM 1381 (11).…”

Section: Separation and Determination Of Glycolic Acid In Cell Lysatementioning

confidence: 67%

“…However, the method for determination of glycan structure so far reported is considerably time-consuming and laborious. The procedure involves preparation of a purified cell wall, enzymic fragmentation, gel filtration, and chromatographic or mass spectrometric identification (7)(8)(9)(10)(11).…”

mentioning

confidence: 99%

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Acyl Type of Bacterial Cell Wall: Its Simple Identification by Colorimetric Method

Uchida¹,

Aida²

1977

J. Gen. Appl. Microbiol.

172

110

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supporting

confidence: 73%

Section: Separation and Determination Of Glycolic Acid In Cell Lysatementioning

confidence: 67%

See 1 more Smart Citation

Acyl Type of Bacterial Cell Wall: Its Simple Identification by Colorimetric Method

Uchida¹,

Aida²

1977

J. Gen. Appl. Microbiol.

172

110

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“…kirovani strain IM 1374 was kindly provided by the Institut Merieux (69 Marcy l'Etoile, France). It was grown in Sauton's medium for one week and the walls were isolated and purified as previously described [6].…”

Section: Wall Preparationmentioning

confidence: 99%

“…The experiments presented herein deal with the characterization of the various wall lipids and with the primary structure of the peptidoglycan of Nocardia strain kirovani. Preliminary reports have appeared [6]. …”

mentioning

confidence: 99%

Structural Investigations on Cell Walls of Nocardia sp.

Vacheron

Guinand

Michel

et al. 1972

European Journal of Biochemistry

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The walls of Nocardia kirovani are composed of three main constituents : the peptidoglycan matrix, a polysaccharide polymer and a variety of free and bound lipids. The free lipids represent 17.5O/, (dry weight) of the walls and consist for the major part of C,,-,, fatty acids and nocardic acids, and for the minor part, of nocardones, triglycerides and carotenoid pigments. The nocardic acids were identified as tri-and tetra-unsaturated, &-branched, 8-hydroxylated compounds C,,H,,,O,-C,,H,,,O,, the nocardones as tri-and tetra-unsaturated ketones C5,HIo60 -C6,H,,,0, and the main carotenoid pigment as phlei-xantophylle palmitate. Esters of glycerol with C,,, C,,, C,, fatty acids and, for some of them, with odd numbered poly-unsaturated acids containing 35 to 45 carbon atoms, were also identified. Bound lipids represent about 20°/, (dry weight) of the walls and consist mainly of nocardic acids probably ester-linked t o an arabinogalactan polymer. The peptidoglycan (about 40°/, dry weight) is composed of ~-1,4-N-acetylglucosaminyl-N-tides, where Azpm is meso-diarninopimelic acid. Crosslinking between some of the peptide units is mediated through D-Ala-(D)-A,pm linkages (peptidoglycan of chemotype I).All bacterial walls contain a peptidoglycan polymer which is basically a network of glycan strands that are interconnected through peptide chains. The glycan moiety consists of linear strands of alternating 8-1,4 linked pyranoside N-acetylglucosamine and N-acetylmuramic acid residues. The muramic acid residues, or a t least some of them, are substituted by tetrapeptide units which consistently have the general sequence L-Ala-D-Glu-L-Res-D-Ala. Most often, the L-Res residue is either a neutral amino acid or a diamino acid such as meso-diaminopimelic acid. The peptide units belonging to adjacent strands are, in turn, cross-linked through peptide bridges. Again, the composition and the location of the bridges vary. Cross-linking between two peptide units, however, always involves the C-terminal D-alanine residue of one of them. I n many Bacillaceae and gram-negative bacteria, for Abbreviations. NMR, nuclear magnetic resonance; aGln, a-glutamine; A,pm, meso-diaminopimelic acid, LL-A,pm, LL isomer of diaminopimelic acid. The notation (L) or (D) written immediate11 before A,pm specifies on which one of the two asymmetric carbons of mesodiaminopimelic acid the substituted amino groups are located. Similarly, the notation (L) or (D) immediately after A,pm distinguishes between the carboxyl-substituted groups.example, the bridging is an interpeptide bond which extends from the C-terminal D-alanine of one peptide unit to the amino group located on the D carbon of meso-diaminopimelic acid of another peptide unit.Most often, the walls of gram-positive bacteria are deprived of lipids and consist of a peptidoglycan matrix to which are covalently attached an almost endless variety of polysaccharides that are frequently negatively charged and sometimes of polyolphosphate polymers that are collectively called teichoic acid [ 11. The gr...

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Characterization of carotenoids in Rhodothermus marinus

et al. 2017

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Rhodothermus marinus, a marine aerobic thermophile, was first isolated from an intertidal hot spring in Iceland. In recent years, the R. marinus strain PRI 493 has been genetically modified, which opens up possibilities for targeted metabolic engineering of the species, such as of the carotenoid biosynthetic pathway. In this study, the carotenoids of the R. marinus type‐strain DSM 4252T, strain DSM 4253, and strain PRI 493 were characterized. Bioreactor cultivations were used for pressurized liquid extraction and analyzed by ultra‐high performance supercritical fluid chromatography with diode array and quadropole time‐of‐flight mass spectrometry detection (UHPSFC‐DAD‐QTOF/MS). Salinixanthin, a carotenoid originally found in Salinibacter ruber and previously detected in strain DSM 4253, was identified in all three R. marinus strains, both in the hydroxylated and nonhydroxylated form. Furthermore, an additional and structurally distinct carotenoid was detected in the three strains. MS/MS fragmentation implied that the mass difference between salinixanthin and the novel carotenoid structure corresponded to the absence of a 4‐keto group on the ß‐ionone ring. The study confirmed the lack of carotenoids for the strain SB‐71 (ΔtrpBΔpurAcrtBI’::trpB) in which genes encoding two enzymes of the proposed pathway are partially deleted. Moreover, antioxidant capacity was detected in extracts of all the examined R. marinus strains and found to be 2–4 times lower for the knock‐out strain SB‐71. A gene cluster with 11 genes in two operons in the R. marinus DSM 4252T genome was identified and analyzed, in which several genes were matched with carotenoid biosynthetic pathway genes in other organisms.

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Structure des parois cellulaires des nocardia. I‐isolement et composition des parois de Nocardia kirovani

Cited by 33 publications

References 15 publications

Acyl Type of Bacterial Cell Wall: Its Simple Identification by Colorimetric Method

Acyl Type of Bacterial Cell Wall: Its Simple Identification by Colorimetric Method

Structural Investigations on Cell Walls of Nocardia sp.

Characterization of carotenoids in Rhodothermus marinus

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