Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis

Goyette-Desjardins, Guillaume; Vinogradov, Evgeny; Okura, Masatoshi; Takamatsu, Daisuke; Gottschalk, Marcelo; Segura, Mariela

doi:10.1016/j.carres.2016.07.005

Cited by 21 publications

(27 citation statements)

References 27 publications

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“…1A and B). Moreover, purified CPS yields of the mutants SS2to3, SS2to7, SS2to8, SS2to9, and SS2to14 were comparable to those previously reported [9, 11–13] (Table S1). Nuclear magnetic resonance (NMR) analyses confirmed the serotype identity for the serotype-switched mutants, except for SS2to9 (Fig.…”

Section: Resultssupporting

confidence: 88%

“…Nuclear magnetic resonance (NMR) analyses confirmed the serotype identity for the serotype-switched mutants, except for SS2to9 (Fig. S5) [9, 11–13]. The CPS of SS2to9 slightly differed from that of serotype 9 strain 1273590 (used for CPS structure determination [11]) in that SS2to9 possessed a glucose instead of a galactose side chain (Fig.…”

Section: Resultsmentioning

confidence: 83%

“…S5) [9, 11–13]. The CPS of SS2to9 slightly differed from that of serotype 9 strain 1273590 (used for CPS structure determination [11]) in that SS2to9 possessed a glucose instead of a galactose side chain (Fig. S6A), suggesting that the donor strain and SS2to9 may be classified as a serotype 9 variant, which reacts with anti-serotype 9 serum (see Text S1 for more detail).…”

Section: Resultsmentioning

confidence: 99%

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Capsular polysaccharide switching inStreptococcus suismodulates host cell interactions and virulence

Okura

Auger

Shibahara

et al. 2020

Preprint

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Streptococcus suis serotype 2 strains can cause severe infections in both swine and humans. The capsular polysaccharide (CPS) of S. suis defines various serotypes based on its composition and structure. Though serotype switching from serotype 2 has been suggested to occur between S. suis strains, its impact on pathogenicity and virulence remains unknown. Herein, we experimentally generated S. suis serotype-switched mutants from a serotype 2 strain (SS2) that express the serotype 3, 4, 7, 8, 9, or 14 CPS (SS2to3, SS2to4, SS2to7, SS2to8, SS2to9, and SS2to14, respectively). The effects of serotype switching were then investigated with regards to classical properties conferred by presence of the serotype 2 CPS, including adhesion to/invasion of porcine tracheal epithelial cells, resistance to phagocytosis by murine macrophages, killing by murine and porcine whole blood, and dendritic cell-derived pro-inflammatory mediator production. Results demonstrated that these properties on host cell interactions were differentially modulated depending on the switched serotypes. Using a mouse model of systemic infection, SS2to8 was demonstrated to be hyper-virulent, with animals rapidly succumbing to septic shock, whereas SS2to3 and SS2to4 were less virulent than SS2 because of a reduced systemic inflammatory host response. By contrast, switching to serotype 7, 9, or 14 CPSs had little to no effect. Finally, development of clinical signs in a porcine model of infection was only observed following infection with SS2, SS2to7, and SS2to8. Taken together, these findings suggest that serotype switching can differentially modulate S. suis host cell interactions and virulence depending on the CPS type expressed.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

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Capsular polysaccharide switching inStreptococcus suismodulates host cell interactions and virulence

Okura

Auger

Shibahara

et al. 2020

Preprint

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“…The functions of HG124 and HG125 were related to restriction-modification system, while those of HG126, HG127, and HG128 were unknown. Recently, the chemical composition and structure of type 1 CPS was reported [ 58 ]; further analysis of the structure of a type 2 cps loci may allow to assess the role of HG124, HG125, HG126, HG127, and HG128. The fact that the reference strain harbors a type 2 cps locus also confirms that this strain is rather atypical.…”

Section: Discussionmentioning

confidence: 99%

Genomic comparisons of Streptococcus suis serotype 9 strains recovered from diseased pigs in Spain and Canada

Zheng¹,

Du²,

Qiu³

et al. 2018

Vet Res

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Streptococcus suis is one of the most important bacterial pathogens in the porcine industry and also a zoonotic agent. Serotype 9 is becoming one of the most prevalent serotypes within the S. suis population in certain European countries. In the present study, serotype 9 strains isolated from a country where infection due to this serotype is endemic (Spain), were compared to those recovered from Canada, where this serotype is rarely isolated from diseased pigs. For comparison purposes, strains from Brazil and the only strain isolated from a human case, in Thailand, were also incorporated. Firstly, sequence types (STs) were obtained followed by detection of putative virulence factors. Phylogenetic trees were constructed using the non-recombinant single nucleotide polymorphisms from core genomes of tested strains. Most Spanish strains were either ST123 or ST125, whereas Canadian strains were highly heterogeneous. However, the distribution of putative virulence factors was similar in both groups of strains. The fact that ST16 strains harbored more putative virulence genes and shared greater similarity with the genome of human serotype 2 strains suggests that they present a higher zoonotic and virulence potential than those from Canada and Spain. More than 80% of the strains included in this study carried genes associated with resistance to tetracycline, lincosamides and macrolides. Serotype 9 strains may be nearly 400 years old and have evolved in parallel into 2 lineages. The rapid population expansion of dominant lineage 1 occurred within the last 40 years probably due to the rapid development of the porcine industry.Electronic supplementary materialThe online version of this article (10.1186/s13567-017-0498-2) contains supplementary material, which is available to authorized users.

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“…For serotypes 7, 8, and 9, the water method was used (29)(30)(31). Briefly, the bacterial pellet from the 6-L culture was resuspended in deionized water (ddH2O).…”

Section: S Suis Capsular Polysaccharidesproduction and Purificationmentioning

confidence: 99%

Comparative Study of Immunogenic Properties of Purified Capsular Polysaccharides fromStreptococcus suisSerotypes 3, 7, 8, and 9: the Serotype 3 Polysaccharide Induces an Opsonizing IgG Response

et al. 2020

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Streptococcus suis is an encapsulated bacterium and one of the most important swine pathogens and a zoonotic agent for which no effective vaccine exists. Bacterial capsular polysaccharides (CPSs) are poorly immunogenic, but anti-CPS antibodies are essential to the host defense against encapsulated bacteria. In addition to the previously known serotypes 2 and 14, that are non-immunogenic, we have recently purified and described the CPS structures for serotypes 1, 1/2, 3, 7, 8, and 9. Here, we aimed to elucidate how these new structurally diverse CPSs interact with the immune system to generate anti-CPS antibody responses. CPS-stimulated dendritic cells produced significant levels of C–C motif chemokine ligand (CCL) 3, partially via Toll-like receptor (TLR) 2- and myeloid differentiation factor 88-dependent pathways, and CCL2, via TLR-independent mechanisms. Mice immunized with purified serotype 3 CPS adjuvanted with TiterMax Gold® produced an opsonizing IgG response, whereas other CPSs or adjuvants were negative. Mice hyperimmunized with heat-killed S. suis serotypes 3 and 9 both produced anti-CPS type 1 IgGs, whereas serotypes 7 and 8 remained negative. Also, mice infected with sublethal doses of S. suis serotype 3 produced primary anti-CPS IgM and IgG responses, of which only IgM were boosted after a secondary infection. In contrast, mice sublethaly infected with S. suis serotype 9 produced weak anti-CPS IgM and IgG responses following a secondary infection. This study provides important information on the divergent evolution of CPS serotypes with highly different structural and/or biochemical properties within S. suis and their interaction with the immune system.

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Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis

Cited by 21 publications

References 27 publications

Capsular polysaccharide switching inStreptococcus suismodulates host cell interactions and virulence

Capsular polysaccharide switching inStreptococcus suismodulates host cell interactions and virulence

Genomic comparisons of Streptococcus suis serotype 9 strains recovered from diseased pigs in Spain and Canada

Comparative Study of Immunogenic Properties of Purified Capsular Polysaccharides fromStreptococcus suisSerotypes 3, 7, 8, and 9: the Serotype 3 Polysaccharide Induces an Opsonizing IgG Response

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