Structure elucidation of a major fucopyranose-rich heteropolysaccharide (STP-II) from Sargassum thunbergii

Luo, Dianhui; Yuan, Xiumei; Zeng, Yawei; Nie, Kaiying; Li, Zhiming; Wang, Zhaojing

doi:10.1016/j.carbpol.2016.01.049

Cited by 25 publications

(15 citation statements)

References 29 publications

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“…After hydrolysis for 10 and 15 min, the shape of IR spectrums remarkably changed and moved toward higher elution times (25-45 min) indicating the successful degradation of fucoidan. As shown in Table 1, the weight average molecular weight (M w ) of native fucoidan was found to be 421 9 10 3 g/mol which was in the wide range of 47-987 9 10 3 g/mol reported for the fucoidans from S. thunbergii, S. binderi, S. fusiforme and S. pallidum (Luo et al 2016;Lim et al 2016;Chen et al 2016;Li et al 2017). The significant variation in the molecular weight of fucoidans could be driven by differences in species, growth environment and extraction methods (Tabarsa et al 2015).…”

Section: Resultsmentioning

confidence: 84%

Improved immunomodulatory and antioxidant properties of unrefined fucoidans from Sargassum angustifolium by hydrolysis

et al. 2017

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The effect of polymer degradation was studied on immunomodulatory and antioxidant properties of fucoidan isolated from . Partially hydrolyzed fucoidans were prepared using 0.01 N hydrochloric acid after incubation for 10 and 15 min in boiling water. FT-IR analysis showed two major peaks at 850 cm corresponding to bending vibration of C-O-S of sulfate and 1256 cm derived from the stretching vibration of S-O. The native fucoidan consisted mainly of carbohydrate (49.4%), sulfate (22.9%), uronic acid (10.3%) and minor amount of protein (4.1%). The hydrolysis reduced the molecular weight of native fucoidan from 421 × 10 g/mol to 104.1 × 10 g/mol after 10 min boiling and 63.9 × 10 g/mol after 15 min boiling, without a significant change in their chemical compositions. Acid degradation increased the specific volume of gyration from 0.84 to 3.32 cm/g in hydrolyzed fucoidan polymers. Fucoidan with the lowest molecular weight showed the greatest proliferating effect on RAW264.7 cells and induced the macrophage cells to release more nitric oxide (39.0 μmol) at 50 μg/mL. The DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power remarkably increased after hydrolysis. The current results showed that molecular weight has determinant effect on immunomodulatory and antioxidant activities of unrefined fucoidan and thus acid hydrolysis can be applied on commercial scale to obtain fucoidans with more beneficial effects.

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Section: Resultsmentioning

confidence: 84%

Improved immunomodulatory and antioxidant properties of unrefined fucoidans from Sargassum angustifolium by hydrolysis

et al. 2017

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“…Nuclear magnetic resonance spectrum was used to identify the glycosidic linkage of FVP2 based on the monosaccharide composition and anomeric chemical shifts (Bahramzadeh, Tabarsa, You, Li, & Bita, ; Li et al, ; Luo et al, ; Xia, Wang, Yu, Liang, & Kuang, ). It can be seen from the FVP2 hydrogen spectrum, three signals at δ = 4.4 ppm, 4.94 ppm and 5.29 ppm (Figure c), corresponding to the anomeric protons and suggesting three residues identified as (1 → 3,6)‐β‐D‐Gal,(1 → 6)‐α‐D‐Glc and (1 → 3,6)‐α‐D‐Man respectively.…”

Section: Resultsmentioning

confidence: 99%

A novel polysaccharide isolated from Flammulina velutipes, characterization, macrophage immunomodulatory activities and its impact on gut microbiota in rats

Wang

et al. 2020

Animal Physiology Nutrition

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The structural characteristics of a novel Flammulina velutipes polysaccharide (FVP2) were explored in this study. Besides, immunomodulatory activities of FVP2 on RAW 264.7 cell and its impact on gut microbiota in rats were investigated. FVP2 has a molecular weight of 18.3 kD, and its main components include galactose (19.96%), glucose (60.66%) and mannose (19.38%). By NMR analysis, the main‐chain structure consisted of (1 → 3)‐linked‐β‐D‐Gal, (1 → 6) ‐linked‐β‐D‐Gal, (1 → 6)‐linked‐α‐D‐Glc and (1 → 3,6)‐linked‐α‐D‐Man was identified. Results of the in vitro assays on RAW 264.7 murine macrophage cells showed FVP2 could significantly up‐regulate the expression of NO, TNF‐α and IL‐6. FVP2 was intragastrically administered to rats for 2 weeks. Compared with the control group, two caecal short‐chain fatty acids (SCFAs) concentration (isobutyric acid and butyric acid) and the abundance of beneficial microbiota of the FVP2‐treated group were significantly increased (p < .05) respectively. The results demonstrated that FVP2 could effectively enhance the level of butyric acid and increase beneficial gut microbiota, which could improve the intestinal barrier function and maintain the intestinal mucosal integrity, suggesting that FVP2 could potentially be an immunomodulators or a functional food to promote intestinal health.

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“…In 1993, Patankar et al published a revised structure of a commercial fucoidan from F. vesiculosus : mainly an α(1→3)- l -fucose linear backbone with sulfate substitution at O-4 and some α- l -fucose branched at O-4 or O-2 [20]. Thereafter, studies on fucoidans’ structure evidenced different repeating units for highly purified fucoidan fractions from different species [21,22,23,24,25,26,27,28]. Structures are based on an α(1→3)- l -fucose backbone with some alternating α(1→4) linkages.…”

Section: What Are Fucoidans?mentioning

confidence: 99%

Fucoidans in Nanomedicine

et al. 2016

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Fucoidans are widespread cost-effective sulfated marine polysaccharides which have raised interest in the scientific community over last decades for their wide spectrum of bioactivities. Unsurprisingly, nanomedicine has grasped these compounds to develop innovative therapeutic and diagnostic nanosystems. The applications of fucoidans in nanomedicine as imaging agents, drug carriers or for their intrinsic properties are reviewed here after a short presentation of the main structural data and biological properties of fucoidans. The origin and the physicochemical specifications of fucoidans are summarized in order to discuss the strategy of fucoidan-containing nanosystems in Human health. Currently, there is a need for reproducible, well characterized fucoidan fractions to ensure significant progress.

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Structure elucidation of a major fucopyranose-rich heteropolysaccharide (STP-II) from Sargassum thunbergii

Cited by 25 publications

References 29 publications

Improved immunomodulatory and antioxidant properties of unrefined fucoidans from Sargassum angustifolium by hydrolysis

Improved immunomodulatory and antioxidant properties of unrefined fucoidans from Sargassum angustifolium by hydrolysis

A novel polysaccharide isolated from Flammulina velutipes, characterization, macrophage immunomodulatory activities and its impact on gut microbiota in rats

Fucoidans in Nanomedicine

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