Structure, Function, and Motility of Vacuoles in Filamentous Fungi

Cole, Louise; Orlovich, David A.; Ashford, A. E.

doi:10.1006/fgbi.1998.1051

Cited by 118 publications

(109 citation statements)

References 42 publications

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“…Investigators have previously shown that dyes targeted to the vacuole localize in a tubular network near the tips of hyphae (10,24,31,33). The cellular compartments visualized with RFP-VAM-3 protein, a vacuolar SNARE, looked like components of this vacuolar network.…”

Section: Resultsmentioning

confidence: 92%

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Structure and Distribution of Organelles and Cellular Location of Calcium Transporters in Neurospora crassa

et al. 2009

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We wanted to examine the cellular locations of four Neurospora crassa proteins that transport calcium. However, the structure and distribution of organelles in live hyphae of N. crassa have not been comprehensively described. Therefore, we made recombinant genes that generate translational fusions of putative organellar marker proteins with green or red fluorescent protein. We observed putative endoplasmic reticulum proteins, encoded by grp-78 and dpm, in the nuclear envelope and associated membranes. Proteins of the vacuolar membrane, encoded by vam-3 and vma-1, were in an interconnected network of small tubules and vesicles near the hyphal tip, while in more distal regions they were in large and small spherical vacuoles. Mitochondria, visualized with tagged ARG-4, were abundant in all regions of the hyphae. Similarly, we tagged the four N. crassa proteins that transport calcium with green or red fluorescent protein to examine their cellular locations. NCA-1 protein, a homolog of the SERCA-type Ca 2؉ -ATPase of animal cells, colocalized with the endoplasmic reticulum markers. The NCA-2 and NCA-3 proteins are homologs of Ca 2؉ -ATPases in the vacuolar membrane in yeast or in the plasma membrane in animal cells. They colocalized with markers in the vacuolar membrane, and they also occurred in the plasma membrane in regions of the hyphae more than 1 mm from the tip. The cax gene encodes a Ca 2؉ /H ؉ exchange protein found in vacuoles. As expected, the CAX protein localized to the vacuolar compartment. We observed, approximately 50 to 100 m from the tip, a few spherical organelles that had high amounts of tagged CAX protein and tagged subunits of the vacuolar ATPase (VMA-1 and VMA-5). We suggest that this organelle, not described previously in N. crassa, may have a role in sequestering calcium.

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Section: Resultsmentioning

confidence: 92%

“…GFP and fluorescent dyes have been used successfully to examine nuclei and mitochondria (24,26). Several reports have shown that "the vacuole" is far more dynamic and complex than the textbook presentation of a large spherical organelle (10,24,31,33,54). Our understanding of the structure and abundance of the ER and the Golgi body is limited.…”

mentioning

confidence: 99%

Structure and Distribution of Organelles and Cellular Location of Calcium Transporters in Neurospora crassa

et al. 2009

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“…The vacuolar profiles resemble those stained with Oregon Green 488 carboxylic acid diacetate 8 (carboxy-DFFDA) (Uetake et al 2002;Saito et al 2004) that have been used as a selective probe for fungal vacuoles (Cole et al 1998;Uetake et al 2002). A pH-mapping image of tubular vacuoles in a G. margarita germ tube is presented in Fig.…”

Section: Resultsmentioning

confidence: 95%

“…However, the vacuolar system in living filamentous fungi has been revealed as a complex tubular system, not a simple spherical form (Shepherd et al 1993;Rees et al 1994;Uetake et al 2002;Shoji et al 2006;Ashford and Allaway 2007). The vacuoles of ectomycorrhizal (EM) fungi are motile and consist of tubular and spherical forms (Shepherd et al 1993;Cole et al 1998). The motile tubular vacuole system is probably involved in long distance P i translocation Darrah et al 2006).…”

Section: Introductionmentioning

confidence: 99%

pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita

et al. 2014

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“…3C). It is pertinent to note at this point that tubular vacuoles are common in filamentous fungi and are intimately involved in intracellular transport (Cole et al, 1998).…”

Section: Vesicle Involvement In Organic Acid and Suc Secretionmentioning

confidence: 99%

Vesicle-Mediated Solute Transport between the Vacuole and the Plasma Membrane

Echeverrı́a

2000

Plant Physiology

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Structure, Function, and Motility of Vacuoles in Filamentous Fungi

Cited by 118 publications

References 42 publications

Structure and Distribution of Organelles and Cellular Location of Calcium Transporters in Neurospora crassa

Structure and Distribution of Organelles and Cellular Location of Calcium Transporters in Neurospora crassa

pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita

Vesicle-Mediated Solute Transport between the Vacuole and the Plasma Membrane

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