Structure, function and redesign of vanillyl-alcohol oxidase

Heuvel, Robert H. H. van den; Fraaije, Marco W.; Mattevi, Andrea; Berkel, W.J.H. van

doi:10.1016/s0531-5131(02)00272-8

Cited by 11 publications

(7 citation statements)

References 38 publications

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“…Since the enzyme is active with a wide range of phenolic substrates (Van den Heuvel et al 2000Heuvel et al , 2002, it is necessary to measure the relative activity of extracellular broth towards different phenolic substrates and mainly against vanillyl alcohol. The different trends of VAO and laccase activity in Figure 1 indicate that P. simplicissimum H5 produced two different phenolic oxidases.…”

Section: Discussionmentioning

confidence: 99%

Laccase activities of a soil fungus Penicillium simplicissimum in relation to lignin degradation

Zeng

Huang

et al. 2006

World J Microbiol Biotechnol

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The laccase activities of Penicillium simplicissimum H5 during solid-state fermentation with rice straw were studied. Degradation of lignocellulose was also followed. Results showed that all supplemental carbon sources inhibited the laccase activity in different degrees, while suitable concentrations of supplemental nitrogen sources remarkably enhanced the laccase activity. The enhancement of activity by the ordinary laccase inducers 2, 2¢-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and xylidine was not observed in this study. Lignocellulose degradation was improved when laccase activity was relatively low, suggesting a polymerizing function of laccase in lignin degradation by P. simplicissimum.

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Section: Discussionmentioning

confidence: 99%

Laccase activities of a soil fungus Penicillium simplicissimum in relation to lignin degradation

Zeng

Huang

et al. 2006

World J Microbiol Biotechnol

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“…AA4, AA6, AA7 and AA10 were included in the genome. AA4 included vanillyl-alcohol oxidases, which could transform some phenols [41]. Additionally, AA7 enzymes were involved in biotransformation or detoxification of lignocelluloses [42].…”

Section: Carbohydrate-active Enzyme (Cazyme) Annotationmentioning

confidence: 99%

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Lü

Zheng

et al. 2020

Biotechnol Biofuels

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Background: Bamboo, a lignocellulosic feedstock, is considered as a potentially excellent raw material and evaluated for lignocellulose degradation and bioethanol production, with a focus on using physical and chemical pre-treatment. However, studies reporting the biodegradation of bamboo lignocellulose using microbes such as bacteria and fungi are scarce. Results:In the present study, Bacillus velezensis LC1 was isolated from Cyrtotrachelus buqueti, in which the symbiotic bacteria exhibited lignocellulose degradation ability and cellulase activities. We performed genome sequencing of B. velezensis LC1, which has a 3929,782-bp ring chromosome and 46.5% GC content. The total gene length was 3,502,596 bp using gene prediction, and the GC contents were 47.29% and 40.04% in the gene and intergene regions, respectively. The genome contains 4018 coding DNA sequences, and all have been assigned predicted functions. Carbohydrate-active enzyme annotation identified 136 genes annotated to CAZy families, including GH, GTs, CEs, PLs, AAs and CBMs. Genes involved in lignocellulose degradation were identified. After a 6-day treatment, the bamboo shoot cellulose degradation efficiency reached 39.32%, and the hydrolysate was subjected to ethanol fermentation with Saccharomyces cerevisiae and Escherichia coli KO11, yielding 7.2 g/L of ethanol at 96 h. Conclusions:These findings provide an insight for B. velezensis strains in converting lignocellulose into ethanol. B. velezensis LC1, a symbiotic bacteria, can potentially degrade bamboo lignocellulose components and further transformation to ethanol, and expand the bamboo lignocellulosic bioethanol production.

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“…In the yeast Saccharomyces cerevisiae, coniferyl aldehyde is directly converted to ferulic acid, but enzymes involved in this conversion are not known (Adeboye et al, 2015). In the ascomycete Penicillium simplicissimum, vanillyl-alcohol oxidase (VaO) catalyzes the hydroxylation of eugenol to coniferyl alcohol (de Jong et al, 1992;Van den Heuvel et al, 2002). The physiological substrate of VaO has been proposed to be 4-methoxy(methylphenol) and is the only substrate found so far that induces the production of VaO (Fraaije et al, 1997).…”

Section: Coniferyl Alcohol and Eugenol Metabolic Pathwaysmentioning

confidence: 99%

A comparison between the homocyclic aromatic metabolic pathways from plant-derived compounds by bacteria and fungi

Lubbers

Dilokpimol

Visser

et al. 2019

Biotechnology Advances

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Aromatic compounds derived from lignin are of great interest for renewable biotechnical applications. They can serve in many industries e.g. as biochemical building blocks for bioplastics or biofuels, or as antioxidants, flavor agents or food preservatives. In nature, lignin is degraded by microorganisms, which results in the release of homocyclic aromatic compounds. Homocyclic aromatic compounds can also be linked to polysaccharides, tannins and even found freely in plant biomass. As these compounds are often toxic to microbes already at low concentrations, they need to be degraded or converted to less toxic forms. Prior to ring cleavage, the plant-and lignin-derived aromatic compounds are converted to seven central ring-fission intermediates, i.e. catechol, protocatechuic acid, hydroxyquinol, hydroquinone, gentisic acid, gallic acid and pyrogallol through complex aromatic metabolic pathways and used as energy source in the tricarboxylic acid cycle. Over the decades, bacterial aromatic metabolism has been described in great detail. However, the studies on fungal aromatic pathways are scattered over different pathways and species, complicating a comprehensive view of fungal aromatic metabolism. In this review, we depicted the similarities and differences of the reported aromatic metabolic pathways in fungi and bacteria. Although both microorganisms share the main conversion routes, many alternative pathways are observed in fungi. Understanding the microbial aromatic metabolic pathways could lead to metabolic engineering for strain improvement and promote valorization of lignin and related aromatic compounds.

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Structure, function and redesign of vanillyl-alcohol oxidase

Cited by 11 publications

References 38 publications

Laccase activities of a soil fungus Penicillium simplicissimum in relation to lignin degradation

Laccase activities of a soil fungus Penicillium simplicissimum in relation to lignin degradation

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

A comparison between the homocyclic aromatic metabolic pathways from plant-derived compounds by bacteria and fungi

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