Structure–Function Relationship of the Plant Photosynthetic Pigment–Protein Complex LHCII Studied with Molecular Spectroscopy Techniques

Gruszecki, Wiesław I.

doi:10.1016/b978-0-12-416596-0.00003-8

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…The fluorescence spectra and fluorescence intensity decays were measured upon front face excitation (magic angle mode) with a universal spectrofluorometer (laser LDH-D-C-470-Picoquant, Germany; photomultiplier-Hamamatsu H10721P-01, Hamamatsu Photonics K.K., Japan; monochromator-Shamrock 303i-B, Andor Technology, UK) constructed in our laboratory [10]. As an excitation source, we used an LDH380 laser emitting pulses of about 288 ps (FWHM) half-width at lambda = 380 nm (Pico-Quant, Germany).…”

Section: Apparatusmentioning

confidence: 99%

“…The knowledge of whether we are dealing with aggregate or monomer form at a given concentration of the active compound is vital as the actual form may alter the pharmacological activity of the compound as well as its ability to penetrate through biological membranes. Molecular aggregates also play a crucial role as energy transmitters from sunlight in many biological systems [9][10][11][12] and their unusual optical properties are frequently studied and used in various fields of nanoscience and nanotechnology such as efficient energy collecting, spectral broadening of the optical response of fluorescent systems and biosensing on plasmonic platforms [13][14][15]. Although, TiO 2 nano-objects have demonstrated toxicity and ecotoxicity [16][17][18][19], its use in the biomedical devices is widespread and in many cases does not seem to lead to serious side effects.…”

Section: Introductionmentioning

confidence: 99%

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The Luminescence of 1,8-Diazafluoren-9-One/Titanium Dioxide Composite Thin Films for Optical Application

et al. 2020

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The investigation of innovative label-free α-amino acids detection methods represents a crucial step for the early diagnosis of several diseases. While 1,8-diazafluoren-9-one (DFO) is known in forensic application because of the fluorescent products by reacting with the amino acids present in the papillary exudate, its application for diagnostic purposes has not been fully investigated. The stabilization of DFO over a transparent substrate allows its complexation with biomolecules for the detection of α-amino acids. In this study, DFO was immobilized into a titanium dioxide (TiO2) matrix for the fluorescence detection of glycine, as a target α-amino acid (a potential marker of the urogenital tract cancers). The DFO/TiO2 composite was characterized by atomic force microscopy, spectroscopic ellipsometry, fluorescence spectroscopy and fluorescence microscopy. The performed fluorescent studies indicate spectacular formation of aggregates at higher concentration. The measurements performed using various fluorescence and microscopic techniques together with the suitable analysis show that the aggregates are able to emit short-lived fluorescence.

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Section: Apparatusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Luminescence of 1,8-Diazafluoren-9-One/Titanium Dioxide Composite Thin Films for Optical Application

et al. 2020

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“…Numerous studies have shown that fine adjustments of the photosynthetic functions to different environmental conditions depend largely on the structural flexibility of thylakoid membranes [1,[17][18][19][20][21][22][23][24]. Albeit details of the molecular mechanisms are still under debate, NPQ mechanisms have been thoroughly documented to be associated with structural changes in the thylakoid membranes.…”

Section: Introductionmentioning

confidence: 99%

Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching

et al. 2020

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Non-photochemical quenching (NPQ) is an important photoprotective mechanism in plants and algae. Although the process is extensively studied, little is known about its relationship with ultrastructural changes of the thylakoid membranes. In order to better understand this relationship, we studied the effects of illumination on the organization of thylakoid membranes in Monstera deliciosa leaves. This evergreen species is known to exhibit very large NPQ and to possess giant grana with dozens of stacked thylakoids. It is thus ideally suited for small-angle neutron scattering measurements (SANS)—a non-invasive technique, which is capable of providing spatially and statistically averaged information on the periodicity of the thylakoid membranes and their rapid reorganizations in vivo . We show that NPQ-inducing illumination causes a strong decrease in the periodic order of granum thylakoid membranes. Development of NPQ and light-induced ultrastructural changes, as well as the relaxation processes, follow similar kinetic patterns. Surprisingly, whereas NPQ is suppressed by diuron, it impedes only the relaxation of the structural changes and not its formation, suggesting that structural changes do not cause but enable NPQ. We also demonstrate that the diminishment of SANS peak does not originate from light-induced redistribution and reorientation of chloroplasts inside the cells.

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Effects of polystyrene nanoplastics with different functional groups on the accumulation and toxicity of Pb on dandelion

et al. 2023

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Structure–Function Relationship of the Plant Photosynthetic Pigment–Protein Complex LHCII Studied with Molecular Spectroscopy Techniques

Cited by 5 publications

References 23 publications

The Luminescence of 1,8-Diazafluoren-9-One/Titanium Dioxide Composite Thin Films for Optical Application

The Luminescence of 1,8-Diazafluoren-9-One/Titanium Dioxide Composite Thin Films for Optical Application

Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching

Effects of polystyrene nanoplastics with different functional groups on the accumulation and toxicity of Pb on dandelion

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