Structure of a minimal photosystem I from the green alga Dunaliella salina

Perez-Boerema, Annemarie; Klaiman, Daniel; Caspy, Ido; Netzer-El, Sigal Yoli; Amunts, Alexey; Nelson, Nathan

doi:10.1038/s41477-020-0611-9

Cited by 50 publications

(43 citation statements)

References 52 publications

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“…Among the 16 FCPI subunits, the positions of Fcpa1, Fcpa5, Fcpa6, and Fcpa7 are similar to those of four LHCR subunits in the red algal PSI-LHCI structures 9,10 ( Supplementary Fig. 3a), and the positions of Fcpa1, Fcpa4, Fcpa5, Fcpa6, and Fcpa7 are similar to those of five LHCI subunits in the green algal PSI-LHCI structures [5][6][7][8] ( Supplementary Fig. 3b).…”

Section: Resultsmentioning

confidence: 67%

“…The diatom PSI core contains well conserved, seven trans-membrane subunits (PsaA, PsaB, PsaF, PsaI, PsaJ, PsaL, and PsaM) and three stromal subunits (PsaC, PsaD, and PsaE) ( Supplementary Fig. 4, 5), and its structure is similar to that of cyanobacterial, algal, and higher plant PSI cores [3][4][5][6][7][8][9][10]16 . However, the diatom PSI core lacked PsaG, PsaH, PsaK, and PsaO subunits, among which, PsaK is present in all PSI cores from cyanobacteria to higher plants, PsaG and PsaH are unique to green lineage organisms, and PsaO is found in eukaryotic oxyphototrophs.…”

Section: Resultsmentioning

confidence: 99%

“…3) [3][4][5][6][7][8][9][10] . In the green lineage, four LHCI subunits are found in the plant PSI-LHCI 3,4 , whereas four-ten LHCI subunits are found in the green algal PSI-LHCI [5][6][7][8] . By contrast, three or five LHCR subunits are found in the red algal PSI-LHCI 9,10 .…”

Section: Discussionmentioning

confidence: 99%

“…In order to elucidate the mechanisms of light-energy capture and transfer within the photosystem-LHC supercomplexes, their structural information is indispensable and will also provide insights into why the color variations occur in the oxyphototrophs. The structures of green lineage PSI-LHCI supercomplexes have been determined from higher plants by X-ray crystallography 3,4 and from green algae by cryo-electron microscopy (cryo-EM) [5][6][7][8] . These structural analyses revealed the complicated pigment-protein networks in the PSI-LHCI, offering a structural basis for the energy flow from LHCI to PSI in the green lineages.…”

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confidence: 99%

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Structural basis for assembly and function of a diatom photosystem I-light-harvesting supercomplex

et al. 2020

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Photosynthetic light-harvesting complexes (LHCs) play a pivotal role in collecting solar energy for photochemical reactions in photosynthesis. One of the major LHCs are fucoxanthin chlorophyll a/c-binding proteins (FCPs) present in diatoms, a group of organisms having important contribution to the global carbon cycle. Here, we report a 2.40-Å resolution structure of the diatom photosystem I (PSI)-FCPI supercomplex by cryo-electron microscopy. The supercomplex is composed of 16 different FCPI subunits surrounding a monomeric PSI core. Each FCPI subunit showed different protein structures with different pigment contents and binding sites, and they form a complicated pigment-protein network together with the PSI core to harvest and transfer the light energy efficiently. In addition, two unique, previously unidentified subunits were found in the PSI core. The structure provides numerous insights into not only the light-harvesting strategy in diatom PSI-FCPI but also evolutionary dynamics of light harvesters among oxyphototrophs.

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Section: Resultsmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Structural basis for assembly and function of a diatom photosystem I-light-harvesting supercomplex

et al. 2020

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“…The culture flasks (batch culture) of D. parva were placed on shelves illuminated by fluorescent lamps. The light intensity was adjusted to (25,40, and 70 µ mol m -2 s -1 ) by changing the distance from fluorescent lamps. The light intensity was measured using LI-185 B Quantum/Radiometer photometer.…”

Section: Effect Of Light Intensitymentioning

confidence: 99%

The Influence of Different Light Wavelengths on Growth, Enzymes Activity and Photosynthesis of the Marine Microalga Dunaliella parva W.Lerche 1937

2021

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Light is an important factor that influences the growth and photosynthetic efficiency of microalgae; however, little is known about how light intensity together with the wavelength affect the photosynthetic capacity and growth of marine microalgae. In the present study, the growth of the marine green microalga Dunaliella parva was studied and optimized under different light intensities (25 ~ 70 μmol m-2 s-1) and qualities (blue, green, and red) in comparison with white light at 40 μmol m-2 s-1 as a control. The growth was monitored by counting the cell number, pigment content, Chl a, Chl b, and carotenoids concentrations. The optimal growth and highest photosynthetic efficiency (Fv/Fm) were recorded at a light intensity of 40 μ mol m-2 s-1 , white light, and 1.25 M NaCl (1.47 and 0.678×10 6 cell mL-1 , respectively). The activity of antioxidant enzymes, including catalase and peroxidase, as well as ascorbate content, showed the highest values of 0.190 µM/min.mg Chl, 0.434 and 13.3 mg/g f.wt. respectively, under the green light, which confirmed the presence of environmental stresses.

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A Multifunctional Tyrosine‐Immobilized PAH Molecule as a Universal Cathode Interlayer Enables High‐Efficiency Inverted Polymer Solar Cells

Kim

Moon

Lee

et al. 2021

Advanced Optical Materials

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Structure of a minimal photosystem I from the green alga Dunaliella salina

Cited by 50 publications

References 52 publications

Structural basis for assembly and function of a diatom photosystem I-light-harvesting supercomplex

Structural basis for assembly and function of a diatom photosystem I-light-harvesting supercomplex

The Influence of Different Light Wavelengths on Growth, Enzymes Activity and Photosynthesis of the Marine Microalga Dunaliella parva W.Lerche 1937

A Multifunctional Tyrosine‐Immobilized PAH Molecule as a Universal Cathode Interlayer Enables High‐Efficiency Inverted Polymer Solar Cells

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