Structure of a rhamnolipid from Pseudomonas aeruginosa

Edwards, John; Hayashi, James A.

doi:10.1016/0003-9861(65)90204-3

Cited by 154 publications

(48 citation statements)

References 18 publications

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“…The same explanation may apply to the traces of rhamnose identified in the slime hydrolysates, since these two sugars have been identified in cell-wall fractions of P. aeruginosa (Salton, 1964;Clarke, Gray & Reaveley, 1967). In addition, aging cultures of P. aerugino8a produce a rhamnosecontaining glycolipid (Jarvis & Johnson, 1949;Edwards & Hayashi, 1965) and this may have been produced in small amounts by the cultures from which the slime was extracted, contributing to the rhamnose identified in the hydrolysates.…”

Section: Quantitative Analysismentioning

confidence: 86%

Composition of Pseudomonas aeruginosa slime

Brown

Foster

Clamp

1969

Biochemical Journal

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1. The slime produced by eight strains of Pseudomonas aeruginosa on a number of different media was demonstrated to be qualitatively the same. Small quantitative differences may be occasioned by differences in the extraction procedure, the growth medium or the strain of organism used. 2. The slime was shown to be predominantly polysaccharide with some nucleic acid material and a small amount of protein. 3. The hydrolysed polysaccharide fraction consists mainly of glucose with smaller amounts of mannose. This accounts for some 50–60% of the total slime. In addition, there is some 5% of hyaluronic acid. The nucleic acid material represents approx. 20% of the total weight, and is composed of both RNA and DNA. 4. Minor components are protein, rhamnose and glucosamine, the protein being less than 5% of the total. 5. Hyaluronic acid is produced in greater quantities from nutrient broth than from chemically defined media, and is more firmly attached to the cells than the other components.

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Section: Quantitative Analysismentioning

confidence: 86%

Composition of Pseudomonas aeruginosa slime

Brown

Foster

Clamp

1969

Biochemical Journal

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“…Flaking occurs (see Fig. S1 in the supplemental material) as a result of the inability of the dried and brittle agar to remain adhered to the target plate, a result which can be due to trapped air underneath the growth medium, insufficient matrix saturation of the sample, use of smooth or polished MALDI target plates, certain medium components (e.g., high salt), and microbial molecules (1,5,17,18,22,26,27,34,38,39,59,70). In some cases, sample flaking does not interfere with instrument operation (Fig.…”

Section: Challenges In Microbial Imsmentioning

confidence: 99%

Primer on Agar-Based Microbial Imaging Mass Spectrometry

et al. 2012

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Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) imaging mass spectrometry (IMS) applied directly to microbes on agar-based medium captures global information about microbial molecules, allowing for direct correlation of chemotypes to phenotypes. This tool was developed to investigate metabolic exchange factors of intraspecies, interspecies, and polymicrobial interactions. Based on our experience of the thousands of images we have generated in the laboratory, we present five steps of microbial IMS: culturing, matrix application, dehydration of the sample, data acquisition, and data analysis/interpretation. We also address the common challenges encountered during sample preparation, matrix selection and application, and sample adherence to the MALDI target plate. With the practical guidelines described herein, microbial IMS use can be extended to bio-based agricultural, biofuel, diagnostic, and therapeutic discovery applications.

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“…The Rh1 L-rhamnosyl -L rhamnosylb -hydroxydecanoyl -b -hydroxydecanoate, and L -rhamnosyl -bhydrodecanoyl -b hydroxydecanoate, an Rh2, (Fig. 1) are the principal glycolipid produced by P. aeruginosa [19]. …”

Section: Polymeric Surfactantsmentioning

confidence: 99%

The Definition, Preparation and Application of Rhamnolipids as Biosurfactants

Kamal-Alahmad¹

2015

IJNFS

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Rhamnolipids mainly are produced by Pseudomonas aeruginosa and another microorganism that have been found to have good surface activity. Rhamnolipids are used in various application areas, including environmental, health, food, cosmetic, oil industries, pharmaceuticals and environmental bio remediation particularly in enhanced oil recovery (EOR) and cleaning of oil spills. Many kinds of bio surfactant such as, rhamnolipids are already being used in industry but it is important to develop indigenous technology for the production of rhamnolipids produced by the microorganisms of local origin which would be more suitable for the application to that specific areas. Rhamnolipids have several beneficial uses: they are easily degradable, nontoxic, nonmutagenic, and have the highest surface-tension-reduction index of any surface-tension reducing agent currently in use. In this review, I summarize the definition, preparation, properties, and the application in different areas especially in food and agriculture, and industrial potential of rhamnolipids, as the next generation of biosurfactants.

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Structure of a rhamnolipid from Pseudomonas aeruginosa

Cited by 154 publications

References 18 publications

Composition of Pseudomonas aeruginosa slime

Composition of Pseudomonas aeruginosa slime

Primer on Agar-Based Microbial Imaging Mass Spectrometry

The Definition, Preparation and Application of Rhamnolipids as Biosurfactants

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