2005
DOI: 10.1073/pnas.0506163102
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Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation inEnterococcus faecalis

Abstract: Many bacterial activities, including expression of virulence factors, horizontal genetic transfer, and production of antibiotics, are controlled by intercellular signaling using small molecules. To date, understanding of the molecular mechanisms of peptide-mediated cell-cell signaling has been limited by a dearth of published information about the molecular structures of the signaling components. Here, we present the molecular structure of PrgX, a DNAand peptide-binding protein that regulates expression of the… Show more

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Cited by 121 publications
(202 citation statements)
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“…In uninduced cells, basal transcription from the P Q promoter generates a 380-nt transcript, Q S , which includes a single ORF (prgQ) encoding a 22-aa polypeptide processed into I and secreted into the growth medium (10). An increased intracellular level of C, resulting from addition of exogenous C or C-producing recipient cells, shifts the PrgX structure and oligomerization state from a repressing to a nonrepressing conformation, resulting in induction (11,12). Induced donor cells contain increased levels of Q S , as well as longer transcripts (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…In uninduced cells, basal transcription from the P Q promoter generates a 380-nt transcript, Q S , which includes a single ORF (prgQ) encoding a 22-aa polypeptide processed into I and secreted into the growth medium (10). An increased intracellular level of C, resulting from addition of exogenous C or C-producing recipient cells, shifts the PrgX structure and oligomerization state from a repressing to a nonrepressing conformation, resulting in induction (11,12). Induced donor cells contain increased levels of Q S , as well as longer transcripts (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Once taken up by donor cells, I competes with C for binding to PrgX; the two peptides bind to the same subdomain of PrgX but have opposing effects on PrgX structure and function (11). It has been suggested that in the absence of recipient cells, the basal level expression of I keeps the expression of conjugation genes OFF in donor cells (11,12). When recipient cells are in close proximity, the increased C level overcomes the inhibition of I, thus allowing donor cells to mount a mating response.…”
mentioning
confidence: 99%
“…PrgX binds pCF10 DNA cooperatively at two sequences in the prgX-Q intergenic region (Bae et al, 2002) and recently has been shown to form a DNA loop by binding to both of its operators (Kozlowicz and Dunny, manuscript in preparation). Crystallographic data indicates that PrgX exists as a stable dimer, and likely forms a tetramer while looping the DNA (Shi et al, 2005). Pheromone-binding has previously been shown to disrupt oligomerization, and crystallographic evidence illustrates that this occurs via reduction of the PrgX tetramer to dimers (Shi et al, 2005).…”
Section: The Pheromone-sensitive Switch and Initiation Of A Mating Rementioning
confidence: 96%
“…Crystallographic data indicates that PrgX exists as a stable dimer, and likely forms a tetramer while looping the DNA (Shi et al, 2005). Pheromone-binding has previously been shown to disrupt oligomerization, and crystallographic evidence illustrates that this occurs via reduction of the PrgX tetramer to dimers (Shi et al, 2005). The breaking of tetramers also breaks the DNA loop, and likely reduces the affinity of PrgX for its DNA operator, thus allowing for greater rates of transcription at P Q .…”
Section: The Pheromone-sensitive Switch and Initiation Of A Mating Rementioning
confidence: 98%
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