Structure of plantaricin locus of Lactobacillus plantarum 8P-A3

Tsapieva, Anna N.; Duplik, Nadezhda; Suvorov, Alexander

doi:10.3920/bm2011.0030

Cited by 27 publications

(23 citation statements)

References 16 publications

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“…Different mechanisms of antibacterial action are involved, but synthesis of organic acids and antimicrobial peptides (bacteriocins) are the most common weapons of bacterial wars for colonization locus and for nutrients. Expression of many bacteriocins of lactobacilli, enterococci or bifidobacteria is strictly regulated by the complex genetic regulatory systems involving three-component signaling and pheromone activation by the quorum sensing mechanism [34,35,36]. The majority of bacteriocin-producing strains generate peptides inhibiting growth of a narrow range of bacteria with similar colonization preferences; however, some probiotics such as L. plantarum 8P-A3 or E. faecium L3 synthesize multiple bacteriocins with extremely high inhibitory activities against gram-positive and gram-negative pathogens [35, 36].…”

Section: Mechanisms Of Probiotic Actionmentioning

confidence: 99%

Gut Microbiota, Probiotics, and Human Health

Suvorov

2013

Bioscience of Microbiota, Food and Health

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The review is devoted to the problems of microbiota and the ways of it correction employing beneficial life bacteria- probiotics. It covers the issues related to the functioning of human microbiota and its importance for the health, individual variability of microbial content, functioning of the probiotics in the human organism and the history of probiotic studies with particular focus on the microbiological investigations in the USSR. The article discusses the safety issues related to probiotics and the problems with probiotic therapy, trying to explain the reasons for the side effects caused by probiotics. The necessity of personified selection of the probiotic strain or individual microbial therapy autoprobiotics is also discussed.

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Section: Mechanisms Of Probiotic Actionmentioning

confidence: 99%

Gut Microbiota, Probiotics, and Human Health

Suvorov

2013

Bioscience of Microbiota, Food and Health

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“…plnABCD locus has been known as regulatory operon, and the next three loci (plnEFI, plnJKLR, and plnMNOP) are related to plantaricin biosynthesis and their cognate immunity. plnGHSTUVW locus encodes the ATP-binding cassette (ABC) transporter and suggesting a putative membrane protein, but homology searching with the deduced gene products of these open reading frames (ORFs) showed no obvious sequence similarity to any known proteins [9,10].…”

Section: Introductionmentioning

confidence: 99%

Cloning and Expression of Plantaricin W Produced by Lactobacillus plantarum U10 Isolate from “Tempoyak” Indonesian Fermented Food as Immunity Protein in Lactococcus lactis

Lages

Mustopa

Sukmarini

et al. 2015

Appl Biochem Biotechnol

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Plantaricins, one of bacteriocin produced by Lactobacillus plantarum, are already known to have activities against several pathogenic bacterium. L. plantarum U10 isolated from "tempoyak," an Indonesian fermented food, produced one kind of plantaricin designated as plantaricin W (plnW). The plnW is suggested as a putative membrane location of protein and has similar conserved motif which is important as immunity to bacteriocin itself. Thus, due to study about this plantaricin, several constructs have been cloned and protein was analyzed in Lactococcus lactis. In this study, plnW gene was successfully cloned into vector NICE system pNZ8148 and created the transformant named L. lactis NZ3900 pNZ8148-WU10. PlnW protein was 25.3 kDa in size. The concentration of expressed protein was significantly increased by 10 ng/mL nisin induction. Furthermore, PlnW exhibited protease activity with value of 2.22 ± 0.05 U/mL and specific activity about 1.65 ± 0.03 U/mg protein with 50 ng/mL nisin induction. Immunity study showed that the PlnW had immunity activity especially against plantaricin and rendered L. lactis recombinant an immunity broadly to other bacteriocins such as pediocin, fermentcin, and acidocin.

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“…Pln locus of plantaricin encoding genes is genetically organized in either simple or complex operon (Diep et al, 2009). Plantaricin 423, S and W are found on one simple operon (Nissen-Meyer et al, 2009) whereas, L. plantarum C11, WCFS1, JDM1, J23, J51 and NC8 strains are organized in complex pln locus consisting of 25-28 genes in the mosaiclike structure of 5-6 operons encoding Class IIb bacteriocin (Diep et al, 2009;Tsapieva et al, 2011;Tai et al, 2015). All bacteriocin related information collected from bacteriocin databases, that have been created to compile the increasing number of bacteriocins characterized from both Gram-positive and Gram-negative bacteria, may be used for the automated screening of bacteriocin gene clusters (Blin et al, 2013;Van Heel et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Genome Analysis of Lactobacillus plantarum Isolated From Some Indian Fermented Foods for Bacteriocin Production and Probiotic Marker Genes

2020

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In this study, Lactobacillus plantarum strain DHCU70 isolated from dahi, a fermented milk product and L. plantarum strain DKP1 isolated from kinema, a fermented soybean food of India, respectively were evaluated for their bacteriocin production and probiotic properties. Both strains of L. plantarum (DHCU70 and DKP1) were found to have potent antimicrobial activity against Kocuria rhizophila ATCC 9341. Bacteriocin produced by L. plantarum strains DHCU70 and DKP1 did not exhibit inhibition of cell wall, DNA and fatty acids biosynthesis mechanisms as evaluated by whole cell reporter assays. We characterized the bacteriocin encoding genes in L. plantarum strains DHCU70 and DKP1 by whole genome sequence which consisted of a single and circular chromosome with genome size of 3.38 Mb (GC content of 44.3%) and 3.39 Mb, respectively and a GC content of 44.3%. L. plantarum DHCU70 has 3252 number of protein encoding genes comprising 89 number of RNA genes (69tRNA, 16rRNA, 4nc RNA) whereas L. plantarum DKP1 has total of 3277 number of protein encoding genes with 89 number. of RNA genes (69tRNA, 16S rRNA, 4nc RNA). Analysis revealed the presence of 20.5 kb long and 23 numbers of plantaricin encoding locus (pln locus) for production of antimicrobial compound. BAGEL analysis has shown that the pln locus of both the strains of L. plantarum showed maximum sequence similarity with plantaricin NC8 of L. plantarum NC8, originally isolated from grass silage. Annotated whole genome sequence of both strains DHCU70 and DKP1 was analyzed for the presence of probiotic marker genes. The probiotic properties of these strains of were also evaluated in vitro. Due to the presence of genes responsible for antimicrobial activity and probiotic properties, both strains of L. plantarum may be considered as a suitable probiotic candidate in food industry.

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Structure of plantaricin locus of Lactobacillus plantarum 8P-A3

Cited by 27 publications

References 16 publications

Gut Microbiota, Probiotics, and Human Health

Gut Microbiota, Probiotics, and Human Health

Cloning and Expression of Plantaricin W Produced by Lactobacillus plantarum U10 Isolate from “Tempoyak” Indonesian Fermented Food as Immunity Protein in Lactococcus lactis

Genome Analysis of Lactobacillus plantarum Isolated From Some Indian Fermented Foods for Bacteriocin Production and Probiotic Marker Genes

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