Structure of recombinant mouse collagenase-3 (MMP-13)

C5a mediates secretion and activation of matrix metalloproteinase 9 from human eosinophils and neutrophils

“…1, chemotaxis of eosinophils through Matrigel® could be directed by C3a or C5a, but only C5a was effective for neutrophils. Chemotaxis of both cell types was blocked by a chemical inhibitor of metalloproteinases, RS-113456 [14]. In addition, a mAb against MMP-9 also inhibited anaphylatoxin mediated transmigration of eosinophils (Fig.…”

“…Human recombinant IL-5 was supplied by R&D Systems (Minneapolis, MN), and fluorescein isothiocyanate (FITC-SCN) came from Pierce Chemical Co. (Rockford, IL). The metalloproteinase inhibitor RS-113456 [14] was a gift from Dr. Harold Vanwort, Roche Biosciences, Palo Alto, CA. Dulbecco's modified Eagle's medium (DMEM) (without indicator dye) was from BioWhittaker (Walkersville, MD), and Matrigel® was purchased from Becton Dickinson (Bedford, MA).…”

Parameterization and Classification of the Protein Universe via Geometric Techniques

“…Matrix metalloproteinases (MMPs) belong to a family of zinc dependent endopeptidases, which cleave proteins of the extracellular matrix. These enzymes are attractive therapeutic targets as they are involved in normal physiological processes such as angiogenesis, extracellular matrix remodeling, embryogenesis and differentiation, 31,32 and also in pathological processes such as metastasis, arthritis, and tumor invasion. 33,34 MMPs are characterized by two tetrahedrally coordinated zinc ions, one catalytic and one structural.…”

“…33,34 MMPs are characterized by two tetrahedrally coordinated zinc ions, one catalytic and one structural. 31 The catalytic zinc ion is coordinated by the imidazole nitrogen atoms His201, His205 and His211 and a carboxyl oxygen of Glu202 (1cvx numbering). The structural zinc ion coordinates His151, His166, His179, and the carboxyl oxygen atom of Asp153.…”

Structural and spectroscopic studies of tripodal [MgL] 2+ chelates containing only nitrogen donor atoms: alkaline earth metal complexes as potential drug delivery agents

“…The active sites of MMPs are highly conserved and contain a catalytic zinc center. The first coordination sphere of the Zn(II) ion comprises three imidazoles from His residues and between one and three water molecules [1,[5][6][7][8]. One of the water molecules, commonly known as the catalytic water, is used in hydrolysis of the peptide bond.…”