Structure of stewartan, the capsular exopolysaccharide from the corn pathogen Erwinia stewartii

Nimtz, Manfred; Mort, Andrew J.; Wray, Victor; Domke, Tobias; Zhang, Yongxiang; Coplin, David L.; Geider, Klaus

doi:10.1016/s0008-6215(96)90797-1

Cited by 27 publications

(34 citation statements)

References 8 publications

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“…For example, P. stewartii is note to produce “stewartan” a heteropolymer of glucose and galactose [54]. P. agglomerans KFS-9 produces a heteropolymer constituted by arabinose, glucose galactose and gulcuronic acid with a molecular weight of 760 kDa [55].…”

Section: Resultsmentioning

confidence: 99%

Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide

et al. 2013

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BackgroundMarine environments are the widest fonts of biodiversity representing a resource of both unexploited or unknown microorganisms and new substances having potential applications. Among microbial products, exopolysaccharides (EPS) have many physiological functions and practical applications. Since EPS production by many bacteria is too scarce for practical use and only few species are known for their high levels of production, the search of new high EPS producers is of paramount importance. Many marine bacteria, that produce EPS to cope with strong environmental stress, could be potentially exploited at the industrial level.ResultsA novel bacterium, strain BM39, previously isolated from sediments collected in the Tyrrhenian Sea, was selected for its production of very high levels of EPS. BM39 was affiliated to Pantoea sp. (Enterobacteriaceae) by 16S rRNA gene sequencing and biochemical tests. According to the phylogenetic tree, this strain, being quite far from the closest known Pantoea species (96% identity with P. agglomerans and P. ananatis) could belong to a new species. EPS production was fast (maximum of ca. 21 g/L in 24 h on glucose medium) and mainly obtained during the exponential growth. Preliminary characterization, carried out by thin layer and gel filtration chromatography, showed that the EPS, being a glucose homopolymer with MW of ca. 830 kDa, appeared to be different from those of other bacteria of same genus. The bacterium showed a typical slightly halophilic behavior growing optimally at NaCl 40 ‰ (growing range 0-100 ‰). Flow cytometry studies indicated that good cell survival was maintained for 24 h at 120 ‰. Survival decreased dramatically with the increase of salinity being only 1 h at 280 ‰. The biochemical characterization, carried out with the Biolog system, showed that MB39 had a rather limited metabolic capacity. Its ability, rather lower than that of P. agglomerans, was almost only confined to the metabolization of simple sugars and their derivatives. Few alcohols, organic acids and nitrogen compounds were partially used too.ConclusionsStrain BM39, probably belonging to a new species, due to its remarkable EPS production, comparable to those of known industrial bacterial producers, could be suggested as a new microorganism for industrial applications.

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Section: Resultsmentioning

confidence: 99%

Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide

et al. 2013

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“…Cells of overnight cultures were streaked on a cellophane disk placed on CPG or MM2C agar [10,19]. After 3 d incubation at 28 • C, the cell cultures were suspended in 2 ml water and gently vortexed.…”

Section: Preparation Of Epsmentioning

confidence: 99%

“…Amylovoran consists of five different sugar residues and a glucose residue in a second side chain [9]. Pyrifolan, produced by E. pyrifoliae, and stewartan are related to amylovoran, but the glucose of the second side chain is absent in pyrifolan and a galactose residue in the backbone of stewartan is substituted by glucose [10]. Also the main side chain of stewartan terminates in glucose rather than pyruvate.…”

Section: Introductionmentioning

confidence: 99%

Variations in the molecular masses of the capsular exopolysaccharides amylovoran, pyrifolan and stewartan

Schollmeyer

Langlotz

Huber

et al. 2012

International Journal of Biological Macromolecules

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“…stewartii uses the EsaI/EsaR QS circuitry to regulate stewartan EPS production, necessary for biofilm formation, plant colonization, and virulence. Stewartan EPS is a well-characterized acidic polymer of glucose, galactose and glucuronic acid that enmeshes bacteria in mature biofilms [41,42]. At low cell density and low AHL concentrations, the EsaR QS regulator represses transcription of the stewartan EPS biosynthetic genes and bacteria are competent for attachment to abiotic surfaces [32,43].…”

Section: Epssmentioning

confidence: 99%

“…Furthermore, QS as target for the eradication of biofilms is also discussed. [22,45,47,58,59,[65][66][67] Burkholderia cenocepacia C6-HSL, C8-HSL, BDSF unknown defective in late stages [23,27] Aeromonas hydrophila C4-HSL unknown defective in late stages [9] Pseudomonas putida 3-oxo-C10-HSL, 3-oxo-C12-HSL putisolvin I and II structured biofilm (QS as biofilm repressor) [25,56] Serratia marcescens C4-HSL EPS thin and nonstructured [26,30] Pantoea stewartii 3-oxo-C6-HSL stewartan EPS flat and uniform [32,42,43] Xanthomonas campestris DSF unknown no development or no dispersal [34,35] Xylella fastidiosa DSF unknown no development [17] transcriptional and post-transcriptional level [11]. This highly sophisticated regulatory network, which integrates various environmental parameters, may explain why the influence of QS on biofilm structures was found to be highly dependent on experimental conditions.…”

Section: Introductionmentioning

confidence: 99%

Cell–Cell Communication in Biofilms of Gram‐Negative Bacteria

Aguilar

Carlier

Riedel

et al. 2009

Bacterial Signaling

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Structure of stewartan, the capsular exopolysaccharide from the corn pathogen Erwinia stewartii

Cited by 27 publications

References 8 publications

Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide

Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide

Variations in the molecular masses of the capsular exopolysaccharides amylovoran, pyrifolan and stewartan

Cell–Cell Communication in Biofilms of Gram‐Negative Bacteria

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