Structure of the feline c-fes/fps proto-oncogene: genesis of a retroviral oncogene

Abstract

The nucleotide sequence of the feline c-fes/fps proto-oncogene was analyzed. Comparison with v-fes and v-fps revealed that all v-feslfps homologous sequences were dispersed over 11 kilobase pairs in 19 interspersed segments. All segments, numbered exon 1 to exon 19 as in the chicken and human loci, were flanked by consensus splice junctions. The putative promoter region contained a CATT sequence and three CCGCCC motifs which were also found in the human locus at similar positions. About 200 nucleotides downstream of a translational stop codon in exon 19, a putative poly(A) addition signal was identified. Using the putative translation initiation codon in exon 2, a 93,000-molecular-weight protein could be deduced. This protein resembled very well the putative protein of the human c-feslfps proto-oncogene (94% overall homology) and, although less well, the putative protein of the chicken c-feslfps proto-oncogene (70% overall homology). As far as the feline c-feslfps proto-oncogene sequences transduced to the Gardner-Arnstein (GA) and Snyder-Theilen (ST) strains of feline sarcoma virus (FeSV) are concerned, homology in deduced amino acid sequences between the GA-and ST-v-fes viral oncogenes and the proto-oncogene was 99%. Analysis of the recombination junctions between feline leukemia virus and v-fes sequences in GA-and ST-FeSV proviral DNA revealed for the left-hand junction the involvement of homologous recombination, presumably at the DNA level. The right-hand junction, which appeared identical in the GA-FeSV and ST-FeSV genomes, could have been the result of a site-specific recombination at the RNA level.

Introduction to the special issue on negotiation processes: Modeling frameworks and information technology

“…Dots indicate nucleotides in the various species that are identical to those of the human sequence. The human and cat sequences determined here exactly match the published sequences (Alcalay et al, 1990;Roebroek et al, 1987). The feline sarcoma virus sequence was not determined in this study but is included for comparative purposes.…”

Common mechanism of retrovirus activation and transduction of c-mil and c-Rmil in chicken neuroretina cells infected with Rous-associated virus type 1

“…They were initially identified through their association with retroviruses, acting as natural transducing vectors (38). Generation of replication-defective retroviruses carrying transforming genes proceeds through multiple recombination events and results in alteration of both viral and cellular sequences (3,28,32,37,40). These successive steps during in vivo transduction are difficult to dissect.…”

Structure, origin, and transforming activity of feline leukemia virus-myc recombinant provirus FTT

“…When the v-fes-containing feline sarcoma viruses FeSV-ST and FeSV-GA were examined, the 3' recombination site was found to be identical in both the parental viruses and cellular sequences (24,54), yet the two transducing viruses seem to have arisen separately (10). FeSV-GA (24), FeSV-HZ2 and -HZ4 (containing v-abl and v-kit, respectively) (4,5), and L115 have 5' recombination sites within a 24base-pair region of gag p30.…”