Structure of the lymphocyte membrane. II. Evidence of two classes of Ig on the surface of rabbit lymphocytes during primary response to sheep red blood cells using inhibition of rosette‐forming cells and combined ultrastructural autoradiographic and peroxydase methods

Bona, C; Trebiciavsky, I.; Anteunis, A; Heuclin, C.; Robineaux, R

doi:10.1002/eji.1830020510

Cited by 18 publications

(2 citation statements)

References 28 publications

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“…Normal mouse Ig was also used, and here the activity was 2.05 pCi/,ug protein. Peripheral blood lymphocytes were obtained from a Ficoll-Triosil gradient (Bona et al 1972b) and incubated separately with both iodinated antisera. After incubation ( 1 h at 4' C ) and washing ( X 3 ) , blood films were prepared, and these were used in autoradiography.…”

Section: Methodsmentioning

confidence: 99%

Distribution of Theta Antigens in Ovum Fusion Derived AKR → CBA/H‐T6T6 Tetraparental Mouse Chimaeras

Bona

Tuffrey²,

Barnes³

1974

Tissue Antigens

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The distribution of antigens has been studied on blood lymphocytes of eight AKR++ CBA/ H-ToTa chimaeras using immunoautoradiography and of male hybrids born from the mixed germ line AKR++CBAiH-ToTfi chimaera X Balbic involving the cytotoxicity method. Analysis of 0 positive cells in the peripheral blood of the tetraparental chimaeras showed that the sum of both 0 positive cell populations varied between 49 and 81 % of the total blood lymphocytes. In spite of this, there was a marked variation in the proportion of the two cell populations ~ in some chimaeras ARK 8 positive cells predominated, in others the reverse was true. I n hybrids it is apparent that both antigens were detected, and furthermore, the total percentage exceeded 100 %I. The quantitative distribution of both 0 antigens is held to reflect the contribution of each genotype in the general make-up of the total somatic population and it is concluded that the ovum fusion has not resulted in cell hybridization.

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Section: Methodsmentioning

confidence: 99%

Distribution of Theta Antigens in Ovum Fusion Derived AKR → CBA/H‐T6T6 Tetraparental Mouse Chimaeras

Bona

Tuffrey²,

Barnes³

1974

Tissue Antigens

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“…Analyzes for compatibility determination involve antigenic macromolecules which are common to all lymphocytes. [4]. The macromolecules are distributed on the surface of the lymphocytes allowing processes such as reactions, physicochemical interactions, and adsorption.…”

Section: Introductionmentioning

confidence: 99%

Development of electrochemical biosensor: voltammetric analysis of lymphocytes and indication activation of the complement system

Oliveira¹,

Melo²,

Monte³

et al. 2023

Development and Its Applications in Scientific Knowledge

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This work reports the development of an electrochemical biosensor after immobilization of the lymphocytes to detect the reaction between antibodies and specific HLA antigens present in the serum samples. A clean homemade gold electrode with voltammetric polycrystalline characteristics was used. Lymphocytes were immobilized and tested with positive and negative human serum and complements on the gold electrode. The experiments were carried out in a cell with three electrodes: working - gold, and reference - Ag/AgCl/sat. KCl and auxiliary - platinum. The cyclic voltammetric analyses of immobilized lymphocytes on the gold surface presented an anodic current equal to 1.78 μA at c.a. 0.50 V vs. Ag/AgCl/sat. KCl. The electrochemical responses of the serum (positive and negative) and complement do not show signs of oxidation or reduction in the potential range used. The electrodes with cells and positive serum showed the amplified current signal in the oxidation potential of the cells. The electrode was developed to verify the antigen-antibody reaction, present lymphocyte cells, and human serum samples. The electrode was qualitatively efficient when compared to the methods of flow cytometric analysis and complement-dependent cytotoxicity, being able to be used with operational and economic advantages.

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Immunoglobulin determinants on lymphocytes in germ‐free piglets

et al. 1973

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Surface IgM determinants on splenic lymphocytes were detected in 80-day old pig fetuses using anti-L and anti-p sera conjugated with horseradish peroxidase. Similarly, by the immunofluorsecence technique, only IgM determinants were demonstrated o n unprimed lymphocytes from spleen and bone marrow iin fetuses at the end of gestation (on the 112-1 14th day) and in precolostral, germ-free piglets.The pattern of labeling with anti-p sera is characteristic for nonactivated lymphocytes as documented on fetal splenic lymphocytes o r the small lymphocytes in a dense lymphatic area of the omentum of precolostral piglets. The lymphocytes which showed surface immunofluorescence staining with anti-y serum were observed in conventionally reared, nonimmunized piglets and appeared in all the germ-free reared piglets which were immunized with sheep red blood cells, human serum albumin or bacterial antigens.We conclude that the true antigen recognition units, or "primary" receptors, on antigen-sensitive uncomitted lymphocytes are exclusively of the IgM type and that the IgG-carrying cells can be detected in piglets only after antigenic stimulation.

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Structure of the lymphocyte membrane. II. Evidence of two classes of Ig on the surface of rabbit lymphocytes during primary response to sheep red blood cells using inhibition of rosette‐forming cells and combined ultrastructural autoradiographic and peroxydase methods

Cited by 18 publications

References 28 publications

Distribution of Theta Antigens in Ovum Fusion Derived AKR → CBA/H‐T6T6 Tetraparental Mouse Chimaeras

Distribution of Theta Antigens in Ovum Fusion Derived AKR → CBA/H‐T6T6 Tetraparental Mouse Chimaeras

Development of electrochemical biosensor: voltammetric analysis of lymphocytes and indication activation of the complement system

Immunoglobulin determinants on lymphocytes in germ‐free piglets

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