Structure of triosephosphate isomerase from<i>Cryptosporidium parvum</i>

Nguyen, Trang Nhu; Abendroth, Jan; Leibly, D.J.; Le, Kristen P.; Guo, Wenjin; Kelley, Angela; Stewart, Lance; Myler, Peter J.; Voorhis, Wesley C. Van

doi:10.1107/s1744309111019178

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…Very few were more than 3.0 angstrom that have been studied previously in three different research groups [40][41][42][43] . Out of 77 deposited protein structures of Cryptosporidium retrieved from the Protein Data Bank (PDB), only 16 protein structures had X-Ray resolution value less than 2.0 angstrom [44][45][46][47][48] .…”

Section: Protein Target Selectionmentioning

confidence: 99%

Bioinformatics Applications on Cryptosporidium Research: A Review

Yusof

2022

Bangladesh J Med Sci

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The biology of Cryptosporidium has been studied increasingly since it was recognized as a pathogen of humans more than a century. Its recent recognition as a second leading cause of diarrhoea or cryptosporidiosis immunocompromised patients globally has led many researchers to study on this parasite. Many new technologies such as high-throughput omics and bioinformatics tools have been implemented to investigate this zoonotic parasite in a better approach. The aim of this review article is mainly to briefly describe recent applications of structural bioinformatics in order to reveal the potentiality of a suitable therapeutic target in Cryptosporidium. This review was written based on the search of cited publications in SCOPUS website with the combination of word ‘Cryptosporidium’ with other words like bioinformatics, protein structure, structural biology and homology modeling. The search results then were selected based on the relativeness of updated information needed to be prepared in this review. Several cited publications were used to elaborate the review accordingly despitelimitedreviewupdatesrelatedtoprotein bioinformation of thisparasite. As a conclusion, bioinformatics is a commonly known to be cutting-edge technology that has been recognised for its power to reveal the secret of parasite biology in silico including a neglected parasite, Cryptosporidium. Bangladesh Journal of Medical Science Vol. 21(1) 2022 Page : 8-18

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Section: Protein Target Selectionmentioning

confidence: 99%

Bioinformatics Applications on Cryptosporidium Research: A Review

Yusof

2022

Bangladesh J Med Sci

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“…Nitazoxanide acts against C. parvum by hindering the anaerobic energy transfer reactions through inhibition of the parasitic enzyme pyruvateferredoxin oxidoreductase (Fox and Saravolatz 2005). One of the important enzymes in the glycolysis pathway of C. parvum is triosephosphate isomerase, which ensures maximum ATP production (Nguyen et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Therapeutic efficacy of proton pump inhibitor (omeprazole) on cryptosporidiosis parvum in immunosuppressed experimental mice

et al. 2023

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Cryptosporidiosis is one of the most frequent food and water-borne diseases. The disease might be life-threatening in immunosuppressed patients. Unfortunately, the only approved drug, nitazoxanide, is with variable e cacies, particularly in malnourished children and immunocompromised patients. Therefore, there is a need to discover an alternative treatment that could be achieved by targeting the metabolic pathways. One of the important enzymes in the glycolysis pathway of C. parvum is triosephosphate isomerase, which could be hindered by the proton pump inhibitor (PPI) omeprazole. In this study, omeprazole was repurposed against C. parvum infection in experimentally immunosuppressed mice. This study was conducted on ve mice groups (n = 10). Group I (Normal Control), group II (Infected Control): Mice were infected orally with 1×10 5 C. parvum oocysts on the 15th day of DEX induced immunosuppression. Group III (NTZ-treated): infected and treated by NTZ. Group IV (Omeprazole-treated), and lastly, Group V (NTZ + Omeprazole-treated). The result obtained with omeprazole alone was better than nitazoxanide regarding oocyst shedding reduction percentages (84.9% & 56.1%, respectively). Also, it was better regarding restoration of histopathological and ultrastructural architectures, improvement of liver enzymes (alanine aminotransferase and aspartate aminotransferase) and renal functions (urea and creatinine), and the reduction of C. parvum triosephosphate isomerase (TIM) gene expression by RT-PCR. However, the best results were obtained with the combined treatment. Hence, omeprazole could be considered a novel drug option to treat this life-threatening parasitic infection either alone or combined with NTZ, especially in immunosuppressed patients.

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Cryptosporidium Metabolism

Zhu¹,

Guo²

2013

Cryptosporidium: Parasite and Disease

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Structure of triosephosphate isomerase fromCryptosporidium parvum

Cited by 5 publications

References 32 publications

Bioinformatics Applications on Cryptosporidium Research: A Review

Bioinformatics Applications on Cryptosporidium Research: A Review

Therapeutic efficacy of proton pump inhibitor (omeprazole) on cryptosporidiosis parvum in immunosuppressed experimental mice

Cryptosporidium Metabolism

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