Structured illumination microscopy using extraordinary transmission through sub-wavelength hole-arrays

Docter, M.W.

doi:10.1117/1.2794786

Cited by 12 publications

(5 citation statements)

References 16 publications

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“…State-of-the-art programmable SLMs have emerged as a boon, as these possess excellent capability to produce orientation change, and flexible structured illumination patterns, thereby replacing the use of etched gratings, masks, and interference beams for structured illumination. For some of the SIM techniques based on mask/grating [37,39,66,76,83,150], interference [38,77], the use of diffractive optical elements [171], subwavelength hole arrays [172], and SLMs [79,173,174], the reader is referred to the respective cited articles.…”

Section: Illumination Methodsmentioning

confidence: 99%

Structured illumination microscopy

2015

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Illumination plays an important role in optical microscopy. Köhler illumination, introduced more than a century ago, has been the backbone of optical microscopes. The last few decades have seen the evolution of new illumination techniques meant to improve certain imaging capabilities of the microscope. Most of them are, however, not amenable for wide-field observation and hence have restricted use in microscopy applications such as cell biology and microscale profile measurements. The method of structured illumination microscopy has been developed as a wide-field technique for achieving higher performance. Additionally, it is also compatible with existing microscopes. This method consists of modifying the illumination by superposing a well-defined pattern on either the sample itself or its image. Computational techniques are applied on the resultant images to remove the effect of the structure and to obtain the desired performance enhancement. This method has evolved over the last two decades and has emerged as a key illumination technique for optical sectioning, super-resolution imaging, surface profiling, and quantitative phase imaging of microscale objects in cell biology and engineering. In this review, we describe various structured illumination methods in optical microscopy and explain the principles and technologies involved therein.

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Section: Illumination Methodsmentioning

confidence: 99%

Structured illumination microscopy

2015

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“…The results are indicated per unit area and an average over 50 images is used. Fluorescent images are similar to the ones published elsewhere [15]. The results are compensated for the use of neutral density (ND) filter, exposure time, and illumination light.…”

Section: Measurementsmentioning

confidence: 85%

Fabrication and optical characterization of nano-hole arrays in gold and gold/palladium films on glass

Piciu

Docter

Krogt

et al. 2007

Proceedings of the Institution of Mechanical Engineers, Part N:

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This paper reports on the improved fabrication process and the optical characterization of different nano-hole arrays in thin metal films that are to be integrated into a novel atto-litre titre plate device for high-speed molecular analysis, such as DNA hybridizations and protein immunoassays. The optical detection is based on the enhanced optical transmission that was recently discovered when light passes through periodically distributed sub-wavelength apertures in optically thick metal films. The transmitted light has also small angular diffraction and well-defined spectral features. Using electron-beam (e-beam) lithography (EBL) and lift-off technique, various array structures with hole diameters ranging between 100 nm and 200 nm and different pitches were fabricated in a 200-nm thick layer of gold (Au), palladium (Pd), and gold/palladium (Au/Pd = 60/40) alloy on glass. Introducing Pd to Au, the grain size of the material is decreased, getting a more well-defined shape of the holes. The transmitted spectrum was measured through periodically and randomly distributed nano-holes in Au. Transmitted spectra were compared as well through similar subwavelength hole arrays in Au, Pd, and Au/Pd alloy. Moreover, the fluorescence of Rhodamine G6 (0.05 µM) was measured when using the transmitted light through periodical cavities in Au as the illumination source. It reveals a nine-fold increase in the fluorescent signal.

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“…State-of-the-art programmable SLMs have emerged as a boon, as these possess excellent capability to produce orientation change, and flexible structured illumination patterns, thereby replacing the use of etched gratings, masks and interference beams for structured illumination. For some of the SIM techniques based on mask / grating [37,39,66,76,83,150], interference [38,77], use of diffractive optical elements [171], sub-wavelength hole arrays [172] and SLM [79,173,174] the reader is referred to the respective cited articles.…”

Section: Illumination Methodsmentioning

confidence: 99%

Structured Illumination Microscopy

2016

Super-Resolution Imaging in Biomedicine

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Structured illumination microscopy using extraordinary transmission through sub-wavelength hole-arrays

Cited by 12 publications

References 16 publications

Structured illumination microscopy

Structured illumination microscopy

Fabrication and optical characterization of nano-hole arrays in gold and gold/palladium films on glass

Structured Illumination Microscopy

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