Structures of fd Gene 5 Protein·Nucleic Acid Complexes: A Combined Solution Scattering and Electron Microscopy Study

Tertiary structural changes and iron release from human serum transferrin

“…Guinier (1939) and indirect Fourier transform, or P(r), analyses (Moore, 1980) were used to calculate R g and forward scatter, I(0) for Tf. Detailed descriptions of these analysis procedures are given elsewhere (Heidorn et al, 1989;Trewhella et al, 1990;Olah et al, 1995). For the P(r) analysis, a Q range of 0.012 to 0.18 A Ê À1 was used and the high Q data were assumed to extrapolate as Q À4 in order to reduce truncation errors in the P(r) transformation.…”

“…The instrument resolution function was deconvoluted from the scattering from the Tf in the P(r) analysis. Data analyzed by the Guinier method were not deconvoluted, resulting in apparent R g values smaller by approximately 0.1 to 0.3 A Ê with respect to and within error of the R g values determined by the P(r) analysis method.`R od'' Guinier analysis was used to calculate radius of gyration of cross-section, R c value (Olah et al, 1995;Sosnick et al, 1991). When one dimension of a scattering particle is greater than the other two (e.g.…”

Context Dependence of Mutational Effects in a Protein: The Crystal Structures of the V35I, I47V and V35I/I47V Gene V Protein Core Mutants

“…The high resolution structure of wild-type gene V protein has been determined by X-ray crystallographic analysis (Skinner et al, 1994) and that of the Y41H mutant by X-ray diffraction and four-dimensional NMR methods . In addition, models of gene V protein binding to single-stranded DNA have been proposed (Skinner et al, 1994;Guan et al, 1994Guan et al, , 1995Folmer et al, 1994;Olah et al, 1995). In the present experiments, we have determined the crystal structures of the V35I, I47V, and V35I/I47V mutants of gene V protein in order to help identify the factors that lead to site-dependent effects of core mutations on the stability of this protein.…”

Analyses of the stability and function of three surface mutants (R82C, K69H, and L32R) of the gene V protein from Ff phage by X‐ray crystallography

“…A similar model has been proposed independently using the three-dimensional structure of Y41H GVP determined by NMR Folmer et al, 1994). Such models have been supported by Olah et al (1995) using neutron scattering and modeling.…”

“…During the rolling circle replication process, GVP binds to the ssDNA cooperatively and, ultimately, a superhelical GVP-DNA complex is formed. The structure of such helical complexes has been studied by a number of biophysical methods, including electron microscopy (Gray, 1989;Olah et al, 1995), neutron scattering (Gray et al, 1982;Olah et al, 1995), NMR and fluorescence spectroscopies (King & Coleman, 1987Dick et al, 1989;Stassen et al, 1992;Folkers et al, 1993). The results of these studies have provided valuable insights regarding the interactions of GVP and ssDNA.…”