Structures of Lipopolysaccharide Side‐chains of NCPPB 2995, the Neopathotype Strain of <i>Pseudomonas syringae</i> pv. <i>mors‐prunorum</i> and Three Other Strains, in Relation to O‐serogroup

Spitali, Mariangela; Patel, Mrudula; Smith, Andrew

doi:10.1111/j.1439-0434.1995.tb00221.x

Cited by 9 publications

(34 citation statements)

References 14 publications

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“…Signals at H-6 and C-6 of from 3.87 to 3.81 and 61.1 ppm, respectively, were also consistent with the signals for this residue (see Table S1 three signals were assigned to the three rhamnose linkages (38,39). Weak signals at C and H of 56.6 ppm and 3.44 ppm, respectively, indicated the presence of an O-methyl group, as observed previously for similar molecules (37,39), but the position of this substituent was not investigated further. On the basis of the sugar, glycosyl linkage, and NMR data, Fig.…”

Section: Glycosyl Linkage Analysissupporting

confidence: 67%

“…The other major linkage observed was 4-linked glucopyranose; ␣-1,4-glucan has been reported to be the O-polysaccharide chain from certain strains of Pseudomonas syringae (37,41) and Pseudomonas fluorescens (42). The ratios of rhamnose, fucose, and glucose from the linkage analysis were similar to those determined from the constituent sugar analyses.…”

Section: Glycosyl Linkage Analysissupporting

confidence: 63%

“…␣-D-Glucans, similar to those reported here, have been shown to coextract with lipopolysaccharides from strains of P. syringae pv. morsprunorum (37,41).…”

Section: Fig 11mentioning

confidence: 99%

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Bactericidal Compounds Controlling Growth of the Plant Pathogen Pseudomonas syringae pv. actinidiae, Which Forms Biofilms Composed of a Novel Exopolysaccharide

Ghods

Sims

Moradali

et al. 2015

Appl Environ Microbiol

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c Pseudomonas syringae pv. actinidiae is the major cause of bacterial canker and is a severe threat to kiwifruit production worldwide. Many aspects of the disease caused by P. syringae pv. actinidiae, such as the pathogenicity-relevant formation of a biofilm composed of extracellular polymeric substances (EPSs), are still unknown. Here, a highly virulent strain of P. syringae pv. actinidiae, NZ V-13, was studied with respect to biofilm formation and architecture using a flow cell system combined with confocal laser scanning microscopy. The biofilm formed by P. syringae pv. actinidiae NZ V-13 was heterogeneous, consisting of a thin cellular base layer 5 m thick and microcolonies with irregular structures. The major component of the EPSs produced by P. syringae pv. actinidiae NZ V-13 bacteria was isolated and identified to be an exopolysaccharide. Extensive compositional and structural analysis showed that rhamnose, fucose, and glucose were the major constituents, present at a ratio of 5:1.5:2. Experimental evidence that P. syringae pv. actinidiae NZ V-13 produces two polysaccharides, a branched ␣-D-rhamnan with side chains of terminal ␣-D-Fucf and an ␣-D-1,4-linked glucan, was obtained. The susceptibility of the cells in biofilms to kasugamycin and chlorine dioxide was assessed. About 64 and 73% of P. syringae pv. actinidiae NZ V-13 cells in biofilms were killed when kasugamycin and chlorine dioxide were used at 5 and 10 ppm, respectively. Kasugamycin inhibited the attachment of P. syringae pv. actinidiae NZ V-13 to solid surfaces at concentrations of 80 and 100 ppm. Kasugamycin was bacteriostatic against P. syringae pv. actinidiae NZ V-13 growth in the planktonic mode, with the MIC being 40 to 60 ppm and a bactericidal effect being found at 100 ppm. Here we studied the formation, architecture, and composition of P. syringae pv. actinidiae biofilms as well as used the biofilm as a model to assess the efficacies of bactericidal compounds. P seudomonas syringae pv. actinidiae is the main cause of kiwifruit canker, which is epidemic worldwide (1-3). The P. syringae pv. actinidiae epidemic in New Zealand is threatening the kiwifruit industry, which is the second most important horticultural export crop by monetary value in New Zealand. In New Zealand, the highly virulent strain P. syringae pv. actinidiae V was reported in 2010 in the Bay of Plenty region and caused an estimated 50% loss of vines of the Hort16A kiwifruit variety known as Zespri Gold (4-6). This bacterial disease can affect all commercial species of kiwifruit (3,7,8). The main distinctive symptoms of disease are cankers on the vines and trunks, gummosis, dieback of the canes, wilting, and sometimes death of the vines (5).Similar to other pseudomonads, P. syringae pv. actinidiae can form cell communities known as biofilms. The formation of biofilms is a physiological response to environmental stresses mediated by the regulation of specific sets of genes, including genes encoding virulence factors. Biofilm formation protects cells against host defense me...

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Section: Glycosyl Linkage Analysissupporting

confidence: 67%

Section: Glycosyl Linkage Analysissupporting

confidence: 63%

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Bactericidal Compounds Controlling Growth of the Plant Pathogen Pseudomonas syringae pv. actinidiae, Which Forms Biofilms Composed of a Novel Exopolysaccharide

Ghods

Sims

Moradali

et al. 2015

Appl Environ Microbiol

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“…Most strains of P. syringae that cause canker in stone fruit trees, named variously pv. morsprunorum or syringae, fall either into the serogroup MOP1, characterized by a side‐chain with the structure (Smith et al., 1985b; Spitali et al., 1995): or belong to serogroup MOP2, with the side‐chain as follows (Spitali et al., 1995):…”

Section: Introductionmentioning

confidence: 99%

Structure of the Lipopolysaccharide Side‐chain of Pseudomonas syringae pv. syringae Strain S29

Smith¹

2008

Journal of Phytopathology

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“…In previous papers, we described the side‐chain structures of LPSs from strains of P. syringae pvs. morsprunorum , syringae (Spitali et al., 1995a), aptata , pisi and phaseolicola (Spitali et al., 1995b), representative of the serogroups MOP1 and 2, APT‐PIS and PHA, respectively, in the typing system proposed by Samson and Saunier (1987).…”

Section: Introductionmentioning

confidence: 99%

Structure of the Lipopolysaccharide Side-chain ofPseudomonas syringaepv.tomatoStrain CFBP 2545 in Relation to O-serotype

Spitali

Smith²

2000

Journal of Phytopathology

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The side-chain from the lipopolysaccharide (LPS) of Pseudomonas syringae pv. tomato strain CFBP 2545 contained L-rhamnose. By 1 H-and 13 C-nuclear magnetic resonance spectroscopy, methylation analysis and Smith degradation, the polysaccharide was shown to be a backbone composed of 2,3)±a±L±Rhap(14; 2)±a±L± Rhap(14; and 3)±a±L±Rhap(14 residues with N-acetylfucosamine as a branch residue, primarily of the following structure: * Present address: Celltech-Chiroscience, 216, Bath Road, Slough SL1 4EN, UK.

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Structures of Lipopolysaccharide Side‐chains of NCPPB 2995, the Neopathotype Strain of Pseudomonas syringae pv. mors‐prunorum and Three Other Strains, in Relation to O‐serogroup

Cited by 9 publications

References 14 publications

Bactericidal Compounds Controlling Growth of the Plant Pathogen Pseudomonas syringae pv. actinidiae, Which Forms Biofilms Composed of a Novel Exopolysaccharide

Bactericidal Compounds Controlling Growth of the Plant Pathogen Pseudomonas syringae pv. actinidiae, Which Forms Biofilms Composed of a Novel Exopolysaccharide

Structure of the Lipopolysaccharide Side‐chain of Pseudomonas syringae pv. syringae Strain S29

Structure of the Lipopolysaccharide Side-chain ofPseudomonas syringaepv.tomatoStrain CFBP 2545 in Relation to O-serotype

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