STRUKTUR GENETIK Calliandra calothyrsus DI INDONESIA MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD)

Abstract

Calliandra calothyrsus was introduced to Indonesia from Guatemala in 1930s and had been planted widely throughout Indonesia for fuel wood. Genetic diversity within and among population is an important factor for initiating breeding program. Aim in this study was to evaluate genetic structure of C. calothyrsus in Indonesia, to obtain a better understanding of the history of the distribution and efficiency of breeding strategy of this species. Leaf samples were collected in a seedling seed orchard plot from 10 populations. Six RAPD markers consisted of 34 loci were used to analyze genetic diversity and genetic structure. The results showed that genetic diversity was in low to moderate level (mean HE= 0.321). The AMOVA analysis showed that genetic differentiation among geographical sources was significant, also among populations within geographical sources and among individual within populations as well. It was revealed that the 10 populations originated from only two ancestors. The limited number of ancestors resulted low to moderate genetic distances among populations (mean Da=0.070). In conclusion, C. calothyrsus has moderate genetic diversity within population and weak genetic structure among populations. Genetic diversity in population/individual level should be considered as a selection unit in the breeding strategies.

The application of fertilizers on calliandra (Calliandra calothyrsus Meissn.) seedlings from several populations in West Java

“…The population of calliandra in Indonesia is estimated to only come from 2 areas of origin, which causes low genetic diversity between and within populations [7]. These results are also in line with other types such as Neolamarckia sp.…”

BREEDING FOR BETTER GROWTH OF Calliandra calothyrsus Meissn., A FAST GROWING LEGUME FOR WOOD-ENERGY

“…High genetic gain achieved here, most likely is generated by variations among Calliandra populations collected from throughout Indonesia which was revealed in the progeny test (Nurtjahjaningsih, Sulistyawati & Rimbawanto, 2016). This variation which caused high heritability values (Hendrati, 2015a) might be influenced by different growth habitat on its original population including elevation (Hendrati, 2013).…”

“…Take note that, Calliandra was introduced to Indonesia in 1930 (Nurtjahjaningsih et al, 2016), since then, this species found scattered all around Indonesia might have been able to produce more than 20 generations at particular locations. Due to the ability to adapt to the local condition, these populations might form land races with different genetic structures.…”

Invasive Alien Species Plants in Sukaraja Atas Resort, Bukit Barisan Selatan National Park

“…Menurut Herdiawan dan Sutedi (2015), kaliandra dapat tumbuh di berbagai jenis tanah, termasuk tanah liat berpasir dan tanah masam. Tumbuhan tersebut diintroduksi sejak tahun 1930-an untuk tujaun penyediaan kayu bakar dan pakan ternak, bahkan digunakan sebagai pencegah kebakaran (Herdiawan and Sutedi 2015;Nurtjahjaningsih et al 2016). Menurut Mustabi et al (2019), selain digunakan untuk mencegah kebakaran, ekstrak tumbuhan ini memiliki kandungan zat adiktif yang mampu membunuh cacing seperti cacing kawat (Haemonchus contortus).…”