Studies in African Music

Waterman, Richard A.; Jones, A. M.

doi:10.2307/893220

Cited by 2 publications

(2 citation statements)

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“…The context-dependence of transcriptional enhancement by EBF is reminiscent of that of the pre-B and T cellspecific factor LEF-1/TCF-lIa (Waterman and Jones, 1990;Waterman et al, 1991;Travis et al, 1991a). The context-dependence of transcriptional enhancement by EBF is reminiscent of that of the pre-B and T cellspecific factor LEF-1/TCF-lIa (Waterman and Jones, 1990;Waterman et al, 1991;Travis et al, 1991a).…”

Section: Discussionmentioning

confidence: 97%

A novel lineage-specific nuclear factor regulates mb-1 gene transcription at the early stages of B cell differentiation.

1991

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The mb‐1 gene, which encodes a protein associated with membrane‐bound antibody, is expressed only at the early stages of B cell differentiation. To gain insight into the mechanisms that underlie temporally regulated gene expression, we examined the mb‐1 promoter region for interactions with cell type‐specific DNA binding proteins. Here, we report the characterization of a novel nuclear factor that recognizes the mb‐1 promoter. This DNA binding activity, termed Early B cell Factor, or EBF, is expressed in early stage B cells, but not in late stage B cells, T cells or non‐lymphoid cells. EBF recognizes the nucleotide sequence 5′‐CAAGGGAAT‐3′ in the mb‐1 and major histocompatibility complex (MHC) class II A alpha d promoters. The binding of EBF to DNA was characterized by DNase I footprinting and by methylation interference analysis which indicated both major and minor groove contacts. The specificity of EBF binding is distinct from that of other nuclear factors expressed in hematopoietic cells. EBF appears to consist of at least two polypeptides of approximately 70–75 kDa and 80–85 kDa. The EBF binding site was important for maximal mb‐1 promoter activity in early stage B cells. Moreover, the EBF binding site conferred correct lineage‐ and stage‐specific transcriptional activity upon a heterologous promoter in a context‐dependent manner. Thus, EBF appears to represent an important transcriptional regulator of B cell specific gene expression.

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Section: Discussionmentioning

confidence: 97%

A novel lineage-specific nuclear factor regulates mb-1 gene transcription at the early stages of B cell differentiation.

1991

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“…These enhancer elements are composed of multiple domains that bind T cell-specific and ubiquitous nuclear proteins (Ho et al, 1989;Winoto and Baltimore, 1989a;Gottschalk and Takeda et al, 1990). Interestingly, the purification of some of these factors has shown that a few of them bind to sequences shared by several lymphoid enhancers (Ho et al, , 1991Waterman and Jones, 1990; Marine and Winoto, 1991;Ooesterwegel et al, 1991;Travis et al, 1991;van de Wetering et al, 1991;Waterman et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

TCR beta and TCR alpha gene enhancers confer tissue- and stage-specificity on V(D)J recombination events.

et al. 1993

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We describe transgenic mice carrying germline variable gene segments associated with either the T cell receptor (TCR) beta or alpha gene enhancers (E beta or E alpha). Transgenic constructs underwent high rates of site‐specific rearrangements predominantly in T cells from independent mice. Rearrangements of the E beta‐containing transgenes began at different stages of T cell differentiation in embryonic and adult thymus than did the E alpha‐containing ones, with a pattern superimposable upon the patterns of TCR beta or TCR alpha gene expression, respectively. We demonstrate that sequences within the TCR beta and TCR alpha gene enhancers confer tissue‐ and stage‐specificity upon the V(D)J recombination events affecting adjacent gene segments. The patterns of transgene expression also gave information on developmental events and lineage relationships (gamma delta versus alpha beta) during T cell development.

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Studies in African Music

Cited by 2 publications

References 0 publications

A novel lineage-specific nuclear factor regulates mb-1 gene transcription at the early stages of B cell differentiation.

A novel lineage-specific nuclear factor regulates mb-1 gene transcription at the early stages of B cell differentiation.

TCR beta and TCR alpha gene enhancers confer tissue- and stage-specificity on V(D)J recombination events.

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