Studies in Metabolic Spectra Iv

Abstract

CHENG, LORRAINE (Radiobiochemistry Department, Chas. Pfizer & Co., Inc., Maywood, N. J.) AND J. F. SNELL. Studies in metabolic spectra. IV. Effects of tetracyclines, some of their derivatives, and chloramphenicol on accumulation of glutamic acid in Escwerichia coli. J. Bacteriol. 83:711-719. 1962.-Escherichia coli strain 21 was incubated in the Warburg apparatus at 37 C with sodium acetate-2-C"4 and 0.1 Mmole/ml of various test compounds. Up to 1 hr, de novo C14-glutamic acid (synthesized from the C'4-acetate precursor) accumulation in the fermentation broth was found to be a common phenomenon for the control cells and cells treated with oxytetracycline, chlortetracycline, tetracycline, and chloramphenicol. Subsequently,