Studies of biological functions in blood cells from individuals with large platelets

Breimo, Einar S; Østerud, Bjarne

doi:10.1080/02697450310001632597

Cited by 17 publications

(12 citation statements)

References 35 publications

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“…Our data are consistent with those of Villagra et al who found that the platelets of humans with SCD and pulmonary hypertension had increased sensitivity to activation with ADP and a PAR-1 thrombin receptor activating peptide (62). They did not report platelet size in their patients, but data from other studies in humans have demonstrated that large platelets are functionally more active (63).…”

Section: Discussionmentioning

confidence: 99%

Morphological and functional platelet abnormalities in Berkeley sickle cell mice

Shet

Hoffmann

Jiroušková

et al. 2008

Blood Cells, Molecules, and Diseases

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Berkeley sickle cell mice are used as an animal model of human sickle cell disease but there are no reports of platelet studies in this model. Since humans with sickle cell disease have platelet abnormalities, we studied platelet morphology and function in Berkeley mice (SS). We observed elevated mean platelet forward angle light scatter (FSC) values (an indirect measure of platelet volume) in SS compared to wild type (WT) (37 ± 3.2 vs. 27 ± 1.4, mean ± SD; p <0.001), in association with moderate thrombocytopenia (505 ± 49 × 10 3 /μl vs. 1151 ± 162 × 10 3 /μl; p <0.001). Despite having marked splenomegaly, SS mice had elevated levels of Howell-Jolly bodies and "pocked" erythrocytes (p <0.001 for both) suggesting splenic dysfunction. SS mice also had elevated numbers of thiazole orange positive platelets (5 ± 1 % vs. 1 ± 1%; p <0.001), normal to low plasma thrombopoietin levels, normal plasma glycocalicin levels, normal levels of platelet recovery, and near normal platelet life spans. Platelets from SS mice bound more fibrinogen and antibody to Pselectin following activation with a threshold concentration of a protease activated receptor (PAR)-4 peptide compared to WT mice. Enlarged platelets are associated with a predisposition to arterial thrombosis in humans and some humans with SCD have been reported to have large platelets. Thus, additional studies are needed to assess whether large platelets contribute either to pulmonary hypertension or the large vessel arterial occlusion that produces stroke in some children with sickle cell disease.

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Section: Discussionmentioning

confidence: 99%

Morphological and functional platelet abnormalities in Berkeley sickle cell mice

Shet

Hoffmann

Jiroušková

et al. 2008

Blood Cells, Molecules, and Diseases

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“…Antiplatelet agents have been shown to be effective for prevention of venous thromboembolic disorders, although to a smaller extent than anticoagulants [24,28]. Large platelets have a higher thrombotic potential than small platelets [11], and express higher levels of platelet activation markers, such as P-selectin [12]. Recently, increased levels of P-selectin have been shown in VTE patients [29], and a higher level of circulating P-selectin was associated with increased risk of recurrent VTE in patients with first unprovoked VTE [30].…”

Section: Discussionmentioning

confidence: 99%

“…Increased platelet volume is associated with increased platelet reactivity [9], shortened bleeding time [9], and increased platelet aggregation ex vivo [10]. Large platelets have higher thrombotic potential [11] and express higher levels of P-selectin [12] and glycoprotein IIbIIIa [13] than small platelets. Several studies have shown increased MPV in patients with coronary artery disease [14,15], and MPV has been identified as an independent risk factor for myocardial infarction and stroke [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Mean platelet volume is a risk factor for venous thromboembolism: the Tromsø study

Brækkan¹,

Mathiesen

Njølstad

et al. 2010

Journal of Thrombosis and Haemostasis

282

252

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Summary. Background: Platelet size, measured as mean platelet volume (MPV), is associated with platelet reactivity. MPV is increased in acute myocardial infarction, and has been identified as an independent risk factor for future myocardial infarction and stroke. Objectives: The purpose of the study was to determine the impact of platelet count and MPV on the incidence of venous thromboembolism (VTE) in a prospective, population-based study. Methods: Platelet count, MPV and baseline characteristics were registered in 25 923 subjects aged 25-96 years who participated in the Tromsø Study in 1994-1995. Incident VTE events were registered to the end of followup (1 September 2007). Results: There were 445 validated incident VTE events (1.6 per 1000 person-years), of which 186 (42%) were unprovoked, during a mean of 10.8 years of followup. Subjects with MPV ‡ 9.5 fL had a 1.3-fold [95% confidence interval (CI) 1.0-1.7] higher risk of total VTE and a 1.5-fold (95% CI 1.1-2.3) higher risk of unprovoked VTE than subjects with MPV < 8.5 fL in analyses adjusted for age, sex, smoking, body mass index, and platelet count. Increasing MPV was associated with increased risk of total VTE (P for trend = 0.09) and unprovoked VTE (P for trend = 0.03) in analyses adjusted for age and sex. There was no significant association between increasing platelet count and risk of VTE. Conclusions: An increasing MPV was identified as a predictor for VTE, in particular VTE of unprovoked origin. The present findings support the concept that platelet reactivity is important in the pathogenesis of VTE.

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“…Furthermore, high mean platelet volume (MPV), which indicates platelet activation, has been shown to be an independent risk factor for cardiovascular events [12][13][14][15][16][17]. Plasma viscosity is another relatively easy index of rheology to measure and is also predictive of cardiovascular events [10,11].…”

Section: Introductionmentioning

confidence: 99%

Plasma viscosity and mean platelet volume in patients undergoing coronary angiography

Şenen

Topal²,

Kılınç

et al. 2010

Clinical Hemorheology and Microcirculation

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Background: Markers of platelet activation and haemorrheological indices have been demonstrated to play a role in the pathophysiology of atherosclerosis and cardiovascular events. In this study, we aimed at investigate the association between plasma viscosity and platelet indices in patients undergoing coronary angiography.Materials and methods: Three hundred and eighty four consecutive patients scheduled to undergo coronary angiography were included in the study. Prior to coronary angiography, blood samples were withdrawn to determine routine biochemical markers, blood cell analyses and viscosity measurements. According to the results of coronary angiography, patients were classified either in a subgroup with coronary artery disease (CAD; 1 or more stenoses >50%) or normal coronary arteries (NCA; no stenoses or <50%).Results: There was a statistically significant correlation between plasma viscosity and mean platelet volume levels in all patients undergoing coronary angiography (r = 0.199, p < 0.001). Additionally, when correlation analysis was performed within each group, plasma viscosity significantly correlated with MPV both in patients with CAD (r = 0.18, p = 0.004) and in patients with NCAs (r = 0.272, p = 0.002). Linear regression analysis revealed that plasma viscosity was positively associated with MPV while platelet number was inversely but significantly associated with MPV.Conclusion: We have shown for the first time that MPV correlates with plasma viscosity in patients undergoing coronary angiography, suggesting a relation with plasma proteins and activation of circulating platelets or peripheral consumption of platelets. To evaluate this relation further controlled studies also in patients with acute coronary syndromes are warranted.

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Studies of biological functions in blood cells from individuals with large platelets

Cited by 17 publications

References 35 publications

Morphological and functional platelet abnormalities in Berkeley sickle cell mice

Morphological and functional platelet abnormalities in Berkeley sickle cell mice

Mean platelet volume is a risk factor for venous thromboembolism: the Tromsø study

Plasma viscosity and mean platelet volume in patients undergoing coronary angiography

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