Studies of Budding and Cell Wall Structure of Yeast

Agar, Hilda D.; Douglas, H. C.

doi:10.1128/jb.70.4.427-434.1955

Cited by 55 publications

(8 citation statements)

References 14 publications

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“…It probably also serves as a fracture point which permits the escape of the motile daughter cells from the filament. A somewhat similar structure has been observed between mother and daughter cells in thin sections of yeast (18).…”

Section: Electron Micrograph Of Unsectioned Rhodomicrobium Vannielii supporting

confidence: 74%

FINE STRUCTURE OF THE PHOTOSYNTHETIC BACTERIUM RHODOMICROBIUM VANNIELII

Boatman

Douglas

1961

The Journal of Cell Biology

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The fine structure of the photosynthetic bacterium Rhodomicrobium vannielii was studied by the ultra thin scctioning technique. Cclls were fixed in buffered osmium tctroxide and embedded in Epoxy resin. The feature most common to nearly all cells was an array of intracellular membranes situated in a concentric manner at the periphery of the cell. The membranes were mostly paired and quite often five pairs were seen aligned together. Calculations from densitomctric tracings showed the average width of a "unit" membrane to be 65 A. Sections of material from disrupted cells after passage through a sucrose gradient revcaled vesicular forms composed of membranes similar in width to those in the intact cell. Absorption spectra of both intact cells and isolated membrancs were very similar in the bacteriochlorophyll regions. Septa and membranes were demonstrated in the filaments that join mature cells. No evidence for chromatophores was obtained although the methods used were adequate for their demonstration in Rhodospirillum rubrum.

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Section: Electron Micrograph Of Unsectioned Rhodomicrobium Vannielii supporting

confidence: 74%

FINE STRUCTURE OF THE PHOTOSYNTHETIC BACTERIUM RHODOMICROBIUM VANNIELII

Boatman

Douglas

1961

The Journal of Cell Biology

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“…Consequently, this investigation has shown that in this fungus, the fine structure of the main cellular components is similar to that found already in a great many cells of varied origins. A similar conclusion seems also to be valid for several other fungi (1,16,22,40,47), though, in some cases, technical difficulties of fixation and embedding apparently prevent a clear resolution of structures.…”

Section: Discussionmentioning

confidence: 53%

“…In contrast, the mitochondria in the young plants, the rhizoids, and the hyphae are mostly elongate, and have few cristae which do not penetrate deeply into the matrix of the mitochondrion (Figs. 1,17,19). Cross-sections of what may be tubules and small vesicles are often present in the matrix (Figs.…”

Section: Mitochondriamentioning

confidence: 99%

Relation Between Basophilia and Fine Structure of Cytoplasm in the Fungus Allomyces macrogynus Em

Blondel

Turian

1960

The Journal of Cell Biology

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In a fungus, illomyces macrogynus Em., staining tests have revealed changes in the location of cytoplasmic basophilia following different phases of the developmental cycle. These variations in location were used to observe which fine structures correspond to basophile and non-basophile areas of the cytoplasm.Hyphae, gametangia, zygotes, and plants were fixed at various developmental stages in OsO4, pH 6.1, and embedded in vestopal. Sections were examined in the electron microscope.Comparison of basophile and non-basophile cytoplasms leads to the conclusion that cytoplasmic particles of 150 to 200 A in diameter are responsible for basophilia. The possibility of these particles being ribosomes is discussed and confirmed.The present paper also describes some observations on the fine structure of other cellular components of this fungus, such as nuclei, mitochondria, various granules, and flagella.

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“…Cells from the gypsum slants were fixed, dehydrated, and embedded in methacrylate as previously described (Agar and Douglas, 1955a). Polymerization at both 45 C (Newman et al, 1949) and 80 C (Borysko, 1955) was used.…”

mentioning

confidence: 99%

Studies on the Cytological Structure of Yeast: Electron Microscopy of Thin Sections

1957

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Ultrathin sections of Saccharomyces cerevisiae, grown on Lindegren's presporulation medium, have been studied with the electron microscope. A cytoplasmic membrane, mitochondria, nucleus, central vacuole, storage granules, and an internal double membrane system have been described. The cytoplasmic structures observed resemble closely analogous structures described in thin sections of cells from a variety of other sources.

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Studies of Budding and Cell Wall Structure of Yeast

Cited by 55 publications

References 14 publications

FINE STRUCTURE OF THE PHOTOSYNTHETIC BACTERIUM RHODOMICROBIUM VANNIELII

FINE STRUCTURE OF THE PHOTOSYNTHETIC BACTERIUM RHODOMICROBIUM VANNIELII

Relation Between Basophilia and Fine Structure of Cytoplasm in the Fungus Allomyces macrogynus Em

Studies on the Cytological Structure of Yeast: Electron Microscopy of Thin Sections

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