Studies of Cell Growth on TiO₂ Nanotubes

Abstract

The in vitro cell response was investigated on flat Ti surface vs nanostructured TiO2 nanotube surface. The titanium dioxide nanotube layers were prepared by electrochemical anodization of Ti in ethylene glycol, 5 wt% NH4F and 1ml H2O2. The nanotube layered structure and morphology were characterized using X-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM). The diameter and the length of the nanotubes are found to increase with anodization voltage. Hs27 and breast cancer cell line MCF-7 were used for cell interaction studies. Different surfaces of titanium show variation in term of growth and viability of cells. Different cell type also show different cellular responses to these surfaces. Titanium nanotube with tube diameter 90 nm promoted normal cell adherence and spreading but killed the cancer cells. The detail of the observation is discussed thoroughly in this paper.

The Effect of Initial Etching Sites on the Morphology of TiO2Nanotubes on Ti-6Al-4V Alloy

“…The application of such modification to titanium and its alloys has a positive effect on the bioactivity property of the latter [1]- [3]. This effect has been partly ascribed to the biomimicking of bone nanostructure [4]- [7].…”

“…The following decrease was due to the formation of the barrier layer on the titanium alloy sample (Ti whereby i is the current density, E is the electric field across the oxide layer and U is the voltage across the oxide with thickness, d (1). According to equations (1) and (2), as the thickness of the oxide layer d is increased, the current density would decrease exponentially.…”

Mechano‐cytoskeleton remodeling mechanism and molecular docking studies on nanosurface technology: Titania nanotube arrays

“…8,9 TNA have been described previously with unique surface roughness properties, contact angle, and surface energy that is favorable for cell interaction. [10][11][12][13][14][15] Studies have reported that TNA nanosurface provides better cell spreading and differentiation behavior. Most notably, this study has provided insights into understanding the cell-TNA mechanotransduction response.…”

Localised Delivery of Cisplatin from Chitosan-Coated Titania Nanotube Array Nanosystems Targeting Nasopharyngeal Carcinoma

“…Successful cell attachment and proliferation on the TNA surfaces suggests that the arrays have good biocompatibility and provide a favourable environment for cell growth, as reported in previous studies. 19 , 20 In addition to NPC cells, HEK 293, a human embryonic kidney cell line, was also observed to confirm the biocompatibility of the TNA nanosystem with both normal and cancer cells ( Figure 2a, b ).…”