Studies of drug—protein binding interactions by flow injection analysis with fluorimetric detection

Binding Properties of Hydrophobic Molecules to Human Serum Albumin Studied by Fluorescence Titration

“…These values are in good agreement with the values reported by several authors. [23][24][25][26] Based on the nmax(1) and nmax (2) values thus obtained, we concluded that there are three fluorescence-active ANS binding sites, nsite = 3, in HSA. We previously reported the binding behavior of ANS to BSA, in which identical affinity for four ANS binding sites are expected by the Scatchard analysis.…”

Rapid automated spectrophotometric competitive complexation studies of drugs with cyclodextrins using the flow injection gradient technique: tricyclic antidepressant drugs with α-cyclodextrin

“…Acid dissociation constants, [16][17][18][19] Michaelis-Menten constants, 20 solubility products and critical micellar concentrations 21 have been determined. The binding of fluorescence probes and drugs to proteins 22 and the complexation of micromolecules to b-CD 23 have been studied, and the Cu-EDTA, 24 Cd-glycine, 25 Fe 3+ -salicylate and -thiocyanate and La 3+ -Methyl Thymol Blue 27 complex formation constants have been determined.…”

A method for rapid screening of interactions of pharmacologically active compounds with albumin

“…The cefaclor, etodolac, sulindac, alpha-tris-(hydroxymethyl)-methylamine (tris) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich Chemie (Steinheim, Germany), the 3b-and 3a-aminotropane derivatives (compounds [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] were synthesized at the Department of Drug Technology and Pharmaceutical Biotechnology at the Medical University of Warsaw. Ortho-phosphoric acid (H 3 PO 4 ) was purchased from POCH (Gliwice, Poland).…”

“…Also the flow injection analysis was used for studying association constants. In this method the calibration of the system is required prior to measurements [13][14][15][16]. Each of the presented methods has disadvantages and limitations.…”