Studies of enteric coronaviruses in a feline cell line

Woods, R D

doi:10.1016/0378-1135(82)90059-1

Cited by 10 publications

(3 citation statements)

References 12 publications

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“…Moreover, CCV field strains may vary in their ability to infect different animal hosts and different cell culture systems. Woods andWesley (1986, 1992) were able to infect pigs directly with CCV strain UCD-1 or by contact with dogs infected with an older NVSL challenge strain (DK-1). In contrast, pigs were not infected by CCV strain 1-71 (Binn et al, 1974).…”

Section: Discussionmentioning

confidence: 99%

“…Fetal cat (FC) cells (Woods, 1982) were used to replicate CCV strains and swine testicular (ST) cells (McClurkin and Norman, 1966) were used to replicate TGEV strains. Both the FC and ST cell lines were grown in modified Eagle's minimum essential medium (MEM, Gibco BRL, Gaithersburg, MD) supplemented with fetal bovine serum (FBS, 10%), sodium bicarbonate (0.22%), lactalbumin hydrolysate (0.25%), sodium pyruvate (1×), and gentamicin sulfate (50 mg/ml).…”

Section: Cellsmentioning

confidence: 99%

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The S gene of canine coronavirus, strain UCD-1, is more closely related to the S gene of transmissible gastroenteritis virus than to that of feline infectious peritonitis virus

Wesley

1999

Virus Research

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To gain insight into the genetic relationships among six canine coronavirus (CCV) strains, the variable region of the spike (S) protein gene was sequenced. The CCV strains were: two ATCC reference strains, the Insavc-1 vaccine strain, the National Veterinary Services Laboratories (Ames, IA) challenge strain, and two California field isolates (UCD-1 and UCD-2) from the 1970s. All six strains, downstream of the nucleocapsid (N) protein gene, had sufficient size for an ORF 7b, and thus, none were transmissible gastroenteritis virus (TGEV)-like since TGEV lacks ORF 7b. By sequence analysis of the variable domain at the 5' end of the S gene, five of the six CCV strains had a high degree of identity with feline infectious peritonitis virus (FIPV). However, one CCV field isolate (UCD-1) was different and had a high degree of identity with the 5' end of the TGEV S gene. This suggests that RNA recombination occurred at this site between antigenically related coronaviruses. The low passage field isolates, UCD-1 and UCD-2, varied in their initial infectivity for swine testicular cells suggesting that sequence differences in the variable domain of the S gene may account for biological variation among CCVs.

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Section: Discussionmentioning

confidence: 99%

Section: Cellsmentioning

confidence: 99%

The S gene of canine coronavirus, strain UCD-1, is more closely related to the S gene of transmissible gastroenteritis virus than to that of feline infectious peritonitis virus

Wesley

1999

Virus Research

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“…Some in vivo subtle antigenic differences among FIPV, FECV and other heterologous coronaviruses doses have been described [4,[32][33][34][35]. Although recently developed competitive ELISA using type-specific mAbs may prove useful in distinguishing cats which are infected with virulent FIPV from cats infected with avirulent feline coronaviruses [36], none of the serologic assays routinely used has been specific enough to discern the virus to which a cat had been exposed [9,26,27].…”

Section: Discussionmentioning

confidence: 99%

An enzyme-linked immunosorbent assay using canine coronavirus-infected CRFK cells as antigen for detection of anti-coronavirus antibody in cat

Mochizuki

Furukawa

1989

Comparative Immunology, Microbiology and Infectious Diseases

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From the reasons that canine coronavirus (CCV) grows more efficiently than feline coronavirus in a cell culture and they are mutually related in their antigenicities, an enzyme-linked immunosorbent assay (ELISA) using CCV-infected feline kidney (CRFK) cells as substrate antigens was developed for detection of anti-coronavirus antibodies in cats. It was indispensable for generating coronavirus-specific ELISA antibody activities that the sample was applied to the mock-infected, normal CRFK cells in parallel with the CCV-infected cells and then the optical density values given by the mock-infected cell antigen were subtracted from those given by the virus-infected cell antigen. On the basis of ELISA antibody titers obtained in sera from the cats experimentally infected with CCV and from the spontaneous feline infectious peritonitis (FIP) cases, the ELISA described in the present study was found to be applicable as a simple and easy serologic test which was able to detect anti-coronavirus antibodies as efficiently as the indirect immunofluorescence assay with homologous FIP virus.

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Relatedness of Rabbit Coronavirus to Other Coronaviruses

1987

Coronaviruses

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Rabbit coronavirus (RbCV) was apparently first encountered in 1961 when Scandinavian investigators observed occasional mortality in rabbits used to propagate the Nichols strain of Treponema pallidum. Mortality rates reached 50 percent by 1968 and 75 percent by 1970. Contaminated samples of I. pallidum were brought to the Johns Hopkins University School of Medicine, a World Health Organization center for the study of treponematoses. There it was established that the causative agent was filterable, the heart was the target organ, and the agent was determined by electron microscopy to be a coronavirus. Also, complement fixing antibodies to the human coronaviruses 229E (two way cross) and OC43 (one way cross) were demonstrated in surv1v1ng rabbits. Immunofluorescent staining with anti-229E serum localized fluorescence in the interstitial tissue of the myocardium. Antiserum to RbCV cross reacted with coronaviruses of three other diseases, feline infectious peritonitis (FIPV), canine coronavirus diarrhea (CCV), and transmissible gastroenteritis (TGEV) by radioimmunoassay. In plaque neutralization tests, a slight reduction was observed against TGE and CCV but not against FIP. Antiserum to 229EV, CCV, FIPV and to a lesser degree TGEV partially blocked the clinical course of the disease and reduced mortality. Slight protection was afforded rabbits by vaccination with, in descending order of survivors, CCV, FIPV, and TGEV. Vaccination with calf diarrhea coronavirus (CDCV) provided no protection. M. M. C. Lai et al. (eds.

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Studies of enteric coronaviruses in a feline cell line

Cited by 10 publications

References 12 publications

The S gene of canine coronavirus, strain UCD-1, is more closely related to the S gene of transmissible gastroenteritis virus than to that of feline infectious peritonitis virus

The S gene of canine coronavirus, strain UCD-1, is more closely related to the S gene of transmissible gastroenteritis virus than to that of feline infectious peritonitis virus

An enzyme-linked immunosorbent assay using canine coronavirus-infected CRFK cells as antigen for detection of anti-coronavirus antibody in cat

Relatedness of Rabbit Coronavirus to Other Coronaviruses

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