Studies of Simian Sarcoma and Simian Sarcoma-Associated Virus: I. Analysis of Viral Structural Proteins, and Preparation and Characterization of Antiserum Specific for Viral Envelope Components

Abstract

The structural proteins of simian sarcoma virus type 1 and its associated virus (SSV) were analysed; in general they were similar to the corresponding components of murine leukemia viruses (MuLV), i. e. glycoproteins of approximately 69.000 and 71.000 Daltons (gp69/71), pro­teins corresponding to p15(E) and p12(E) which were identified as envelope, and p31, p15, p12 and p10 proteins identified as internal components. As in MuLV, p12 stained reddish with Coomassie blue but a pl5(C) distinct from p15(E) was not clearly identified. Using antisera specific for individual components of MuLV cross-reactions were observed between the proteins pl5(E), pi2(E) and p31 of SSV and MuLV, and to a minor degree also between their respective major glycoproteins. An antiserum reacting strongly, specifically and almost exclusively with the envelope components of SSV gp69/71, p15 (E) and p12 (E) was prepared in a goat by inoculation of the animal’s own cells, previously transformed in vitro with SSV and grown in goat serum. Comparative studies with this antiserum in complement fixation and Ouchterlony tests confirmed the strong antigenic similarities between SSV and gibbon ape lymphoma virus but did not identify any interspecies reactivity.

Human antibodies reactive with purified envelope antigens of primate type C tumor viruses.

“…Similarly, FLV consists of the spleen focus-forming virus and its helper virus, the lymphoid leukosis virus, which provides the gp7l FLV. For the sake of brevity, the major virus glycoproteins will be designated as gp70 SSV(SSAV) and gp7l FLV. SSV(SSAV) and FLV were grown in HF SSV/JU suspension marmoset cells (42) and Eveline STU-mouse suspension cells (43), respectively, by W. Schafer, V. Moennig, and H.-J. Thiel (Max-Planck-Institute fur Virusforschung, Tfibingen, West Germany).…”

Detection of a recombinant murine leukemia virus-related glycoprotein on virus-negative thymoma cells.

“…51Cr release assays were performed by using rabbit (Pel-Freeze) complement and assorted antisera as described (26). Lactoperoxidasecatalyzed cell surface iodination and metabolic labeling of cells generally followed published procedures (27). The precipitates were analyzed on slab gels (28) and processed by fluorography (29).…”

Monoclonal Antibody Detects a Common Surface Antigen on Two Independently Established Murine Moloney Sarcoma Virus Non-producer Transformants

“…Lactoperoxidase-catalysed cell surface iodination generally followed a published procedure (Deinhardt et al, 1978). Immunoprecipitates were formed as described previously (Deinhardt et al, 1978), analysed on slab gels (Laemmli, 1970), and processed for fluorography according to Bonner & Laskey (1974).…”

“…Immunoprecipitates were formed as described previously (Deinhardt et al, 1978), analysed on slab gels (Laemmli, 1970), and processed for fluorography according to Bonner & Laskey (1974).…”