Studies of supported phospholipid bilayers formed on nanofiltration membranes surface

Wang, Zhining; Zhang, Zhilei; Wang, Xida; Wang, Li; Wang, Miaoqi; Wang, Shuzheng; Jiang, Sheng; Wang, Tao; Liu, Xingchen; Gao, Congjie

doi:10.1080/19443994.2013.768376

Cited by 5 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We speculate that more AqpZ may be incorporated into the positively charged DOPE/DOTAP liposomes, as the zeta potential of proteo-liposomes decreases depending on the amount of AqpZ incorporated. 22 This trend is in accordance with our recent stop-ow results. 30 Table 1 also summarizes the water permeability values.…”

Section: Characteristics Of Liposomes and Proteoliposomessupporting

confidence: 93%

“…By a combined rehydration and extrusion method, as described in our previous work, 0.1 mg mL À1 unilaminar DOPE or DOPE/ DOTAP (R m , mass ratio, ¼ 4/1) liposomes, with a diameter of about 100 nm, were prepared. 22 To prepare AqpZ-containing proteoliposomes, the AqpZ solution was added into the liposome solution, at 1.0 w/v% OG, with a protein-to-lipid mass ratio (P/L) of 1/100. To remove the detergent OG, the mixed solution was dialyzed against a PBS buffer for 3 days.…”

Section: Preparation Of Liposomes and Proteoliposomesmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of an aquaporin-based forward osmosis membrane through covalent bonding of a lipid bilayer to a microporous support

et al. 2015

Self Cite

View full text Add to dashboard Cite

AquaporinZ (AqpZ)-containing, planar, biomimetic membranes hold great application potential in water purification and seawater desalination, due to the excellent permeability and selectivity of AqpZ. However, there remain many challenges to production of robust and defect-free supported lipid bilayer (SLB) biomimetic membranes. By forming amide bonds between the lipid bilayer and microporous substrate, we fabricated an AqpZ-incorporated SLB forward osmosis (FO) membrane, with a large area of 36 cm 2 . With deionized water and 2 mol/L MgCl 2 draw solution, the AqpZ-incorporated biomimetic membrane exhibited a water flux of ~19.2 L·m −2 ·h −1 (LMH) and a reverse solute flux of ~3.2 g·m −2 ·h −1 (gMH). When positively charged phospholipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) was blended in the 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) bilayer, a higher flux (~23.1 LMH) could be reached accompanied with a constant reverse salt flux of 3.1 gMH, due to more AqpZ being embedded in the mixed bilayer. From nanofiltration (NF) test, the water permeability (A) could reach 6.31 LMH/bar with a relative low solute permeability (B) of 1.7 LMH for AqpZ-DOPE/DOTAP SLB membrane. When rinsed by a 0.24 mmol/L TritonX-100 (TX-100) surfactant solution, water flux and reverse salt flux of the biomimetic membrane with covalent bond only slightly increased, whereas the membrane without covalent bonds showed significant increase in both water flux and reverse salt flux after TX-100 treatment.This paper presented an effective method for preparation of biomimetic FO membrane with good separation performance as well as excellent stability and durability. Graphical Abstract IntroductionIn recent decades, forward osmosis (FO) has gradually emerged as one of the most promising water purification and desalination technologies in water supply, energy generation, and food processing. 1-4 Unlike pressure-driven membrane processes, like reverse osmosis (RO) and nanofiltration (NF), FO is a naturallyoccurring osmosis-driven membrane process that takes advantage of the osmotic pressure gradient to impel water across a semipermeable membrane, from the feed solution side with high chemical potential,

show abstract

Section: Characteristics Of Liposomes and Proteoliposomessupporting

confidence: 93%

Section: Preparation Of Liposomes and Proteoliposomesmentioning

confidence: 99%

Fabrication of an aquaporin-based forward osmosis membrane through covalent bonding of a lipid bilayer to a microporous support

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…A DOPC or DOPC/DOTAP mixture with a molar ratio of DOPC/DOTAP = 4/1 was used to prepare the liposome samples. The samples were prepared by using the film rehydration method according to a previously reported protocol . The resulting vesicle solution was extruded 11 times with 100 nm polycarbonate membranes through an extruder system (Avanti Polar Lipids, Alabaster, AL) to obtain unilaminar vesicles with a final concentration of 0.1 mg/mL.…”

Section: Methodsmentioning

confidence: 99%

Layer-by-Layer Assembly of Aquaporin Z-Incorporated Biomimetic Membranes for Water Purification

Wang

et al. 2015

Environ. Sci. Technol.

Self Cite

135

108

View full text Add to dashboard Cite

We fabricated a biomimetic nanofiltration (NF) membrane by immobilizing an Aquaporin Z (AqpZ)-incorporated supported lipid bilayer (SLB) on a layer-by-layer (LbL) complex polyelectrolyte membrane to achieve excellent permeability and salt rejection with a high stability. The polyelectrolyte membranes were prepared by LbL assembly of poly(ethylenimine) (PEI) with positive charges and poly(sodium 4-styrenesulfonate) (PSS) with negative charges alternately on a porous hydrolyzed polyacrylonitrile (H-PAN) substrate. AqpZ-incorporated 1,2-dioleloyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dioleoyl-3-trimethylammo-nium-propane (chloride salt) (DOTAP) vesicles with positive charges were deposited on the H-PAN/PEI/PSS polyelectrolytes membrane surface. The resulting biomimetic membrane exhibited a high flux of 22 L·m(-2)·h(-1) (LMH), excellent MgCl2 rejection of ∼97% and NaCl rejection of ∼75% under an operation pressure of 0.4 MPa. Due to the attractive electrostatic interaction between SLB and the polyelectrolyte membrane, the biomimetic membrane showed satisfactory stability and durability as well as stable NF flux and rejection for at least 36 h. In addition, the AqpZ-containing biomimetic membrane was immersed in a 0.24 mM (critical micellar concentration, CMC) Triton X-100 solution for 5 min. The flux and rejection were slightly influenced by the Triton X-100 treatment. The current investigation demonstrated that the AqpZ-incorporated biomimetic membranes fabricated by the LbL method led to excellent separation performances and robust structures that withstand a high operation pressure for a relatively long time.

show abstract

“…Liposome samples were prepared by the film rehydration method as previously described [ 23 ]. Briefly, a N 2 stream was used to evaporate chloroform from a chloroform solution of DOPC/DOTAP mixture with a molar ratio of DOPC: DOTAP = 4:1 to obtain a lipid film.…”

Section: Methodsmentioning

confidence: 99%

Bio-Inspired Aquaporinz Containing Double-Skinned Forward Osmosis Membrane Synthesized through Layer-by-Layer Assembly

et al. 2015

Self Cite

View full text Add to dashboard Cite

We demonstrated a novel AquaporinZ (AqpZ)-incorporated double-skinned forward osmosis (FO) membrane by layer-by-layer (LbL) assembly strategy. Positively charged poly(ethyleneimine) (PEI) and negatively charged poly(sodium 4-styrenesulfonate) (PSS) were alternately deposited on both the top and bottom surfaces of a hydrolyzed polyacrylonitrile (H-PAN) substrate. Subsequently, an AqpZ-embedded 1,2-dioleloyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dioleoyl-3-trimethylammonium- propane (chloride salt) (DOTAP) supported lipid bilayer (SLB) was formed on PSS-terminated (T-PSS) membrane via vesicle rupture method. The morphology and structure of the biomimetic membranes were characterized by in situ atomic force microscopy (AFM), scanning electron microscope (SEM), Fourier transform infrared spectrometer using the attenuated total reflection technique (ATR-FTIR), and contact angle. Moreover, the FO performance of the resultant membrane was measured by using 2 M MgCl2 solution as draw solution and deionized (DI) water as feed solution, respectively. The membrane with a protein-to-lipid weight ratio (P/L) of 1/50 exhibits 13.2 L/m2h water flux and 3.2 g/m2h reversed flux by using FO mode, as well as 15.6 L/m2h water flux and 3.4 L/m2h reversed flux for PRO mode (the draw solution is placed against the active layer). It was also shown that the SLB layer of the double-skinned FO membrane can increase the surface hydrophilicity and reduce the surface roughness, which leads to an improved anti-fouling performance against humic acid foulant. The current work introduced a new method of fabricating high performance biomimetic FO membrane by combining AqpZ and a double-skinned structure based on LbL assembly.

show abstract

Studies of supported phospholipid bilayers formed on nanofiltration membranes surface

Cited by 5 publications

References 35 publications

Fabrication of an aquaporin-based forward osmosis membrane through covalent bonding of a lipid bilayer to a microporous support

Fabrication of an aquaporin-based forward osmosis membrane through covalent bonding of a lipid bilayer to a microporous support

Layer-by-Layer Assembly of Aquaporin Z-Incorporated Biomimetic Membranes for Water Purification

Bio-Inspired Aquaporinz Containing Double-Skinned Forward Osmosis Membrane Synthesized through Layer-by-Layer Assembly

Contact Info

Product

Resources

About