Studies of the Microbiology of Periodontosis

Newman, Michael G.; Socransky, S. S.; Savitt, Eugene D.; Propas, D.; Crawford, Ann

doi:10.1902/jop.1976.47.7.373

Cited by 375 publications

(184 citation statements)

References 17 publications

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“…Organisms such as Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia or Eikenella corrodens have been considered to have a role in periodontal disease (9,11). Studies have demonstrated a strong correlation between the presence of putative periodontal organisms and the destruction of periodontal tissues (10).…”

Section: Introductionmentioning

confidence: 99%

PCR detection of four periodontopathogens from subgingival clinical samples

Ávila-Campos¹

2003

Braz. J. Microbiol.

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In this study, A. actinomycetemcomitans, B. forsythus, P. gingivalis and F. nucleatum were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. Subgingival clinical samples were collected with sterilized paper points and transported in VMGA III. From all the diluted clinical samples (1:10), DNA was obtained by boiling, and after centrifugation the supernatant was used as template. Specific primers for each bacterial species were used in PCR. PCR amplification was sensitive to identify these organisms. PCR products from each species showed a single band and can be used to identify periodontal organisms from clinical specimens. PCR detection odds ratio values for A. actinomycetemcomitans and B. forsythus were significantly associated with disease showing a higher OR values for B. forsythus (2.97, 95% CI 1.88 -4.70). These results suggest a strong association among the studied species and the periodontal lesion.

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Section: Introductionmentioning

confidence: 99%

PCR detection of four periodontopathogens from subgingival clinical samples

Ávila-Campos¹

2003

Braz. J. Microbiol.

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“…C. gingivalis is thought to form part of the normal oral flora (Holdeman et al, 1985), and is present in both supragingival and subgingival plaque. Members of the genus have been implicated in juvenile periodontal disease (Newman et al, 1976;Slots, 1976) and periodontal disease associated with insulindependent diabetes mellitus (Mashimo e t al., 1983). In mono-infected rats, Capnoytophaga spp.…”

Section: Introductionmentioning

confidence: 99%

Capnocytophaga gingivalis aminopeptidase: a potential virulence factor

1995

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The production and properties of an aminopeptidase from Capnocytophaga gingiwalis were studied. C. gingiveris was grown in continuous culture over a range of dilution rates and the cell-bound and extracellular levels of Frenchay Campus, . Coldharbour Lane, Bristol BS16 lQY, UK aminopeptidase and trypsin-like protease (TLPase) measured. A t high growth rates (06 pm,) TLPase specific activity was low and found exclusively as cellbound activity; at low growth rates (09375 pd), specific activity was high and 26 O/ O was found as extracellular activity. In contrast, aminopeptidase specific activity was highest at 0-3 pm, and the ratio of cell-bound to extracellular activity was relatively constant at all growth rates. Only about 5% of the total activity was extracellular. The aminopeptidase, which has a wide specificity towards artificial substrates, was purified to homogeneity, as judged by SDS-PAGE, from the supernatant fluid of cells grown in continuous culture in a tryptone/glucose!thiamine medium. The enzyme has a molecular mass of 61 kDa, a pl of 63, a pH optimum close to 7.5 and showed a requirement for magnesium or calcium ions. The N-terminal sequence of the first 10 amino acids (Asp-Val-Asn-Met-Leu-Trp-Tyr-Val-x-Arg ...) showed no similarity to any published sequence. This enzyme in its cell-bound or extracellular form may be important in the nutrition and pathogenesis of C. gingiwdis in the human oral cavity.

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“…Both organisms are indigenous to dental plaque, but they are usually present in small numbers (15). Several recent investigations of clinical (21,27,28), serological (33), toxicological (9), and animal model character (11) have implicated A. actinomycetemcomitans as a major pathogen in periodontal destruction, particularly in juveniles. The periopathogenic potential of haemophili is traditionally considered small (25), and H .…”

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confidence: 99%

Notes: Differentiation Between Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by High-Resolution, Two-Dimensional Protein Electrophoresis

Jellum

Tingelstad

Olsen

1984

International Journal of Systematic Bacteriology

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The cellular proteins of eight strains of Actinobacillus actinomycetemcomitans and seven strains of Haemophilus aphrophilus were analyzed by two-dimensional electrophoresis and silver staining. The electrophoretic patterns of A . actinomycetemcomitans and H . aphrophilus were so different that distinction between these closely related bacteria could easily be made. There were also minor quantitative and qualitative differences in the protein patterns of strains within both species, enabling, particularly, A . actinomycetemcomitans strains FDC 2097 and FDC Y4 and H . aphrophilus strains ATCC 19415, FDC 626, and FDC 621 to be singled out.

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Studies of the Microbiology of Periodontosis

Cited by 375 publications

References 17 publications

PCR detection of four periodontopathogens from subgingival clinical samples

PCR detection of four periodontopathogens from subgingival clinical samples

Capnocytophaga gingivalis aminopeptidase: a potential virulence factor

Notes: Differentiation Between Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by High-Resolution, Two-Dimensional Protein Electrophoresis

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