Studies of the role of steroid hormone in the regulation of oocyte maturation in cattle

Wang, Hai Feng; Isobe, Naoki; Kumamoto, Kanako; Yamashiro, Hideaki; Yamashita, Yugo; Takato, Tsuyoshi

doi:10.1186/1477-7827-4-4

Cited by 40 publications

(16 citation statements)

References 38 publications

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“…DHEA and IA are widely used inhibitors of PPP and glycolysis, respectively [35, 36]. Addition of DHEA and IA to the IVM medium significantly inhibited oocyte maturation and subsequent development in mice [1, 37], pigs [2] and cattle [38]. Our results were consistent with these previous studies, as addition of DHEA to the IVM medium significantly decreased the MII-stage rate, while both inhibitors significantly reduced the resultant blastocyst rate.…”

Section: Discussionsupporting

confidence: 91%

The Role of Glucose Metabolism on Porcine Oocyte Cytoplasmic Maturation and Its Possible Mechanisms

et al. 2016

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In the present study, we investigated the potential role of glucose and pyruvate in the cytoplasmic maturation of porcine oocytes by investigating the effect of glucose and/or pyruvate supplementation, in the presence or absence of 10% porcine follicular fluid (PFF), on meiotic maturation and subsequent embryo development. In the absence of 10% PFF, without exogenous addition of glucose and pyruvate, the medium seemed unable to support maturation. In the presence of 10% PFF, the addition of 5.6 mM glucose and/or 2 mM pyruvate during in vitro maturation of cumulus enclosed oocytes increased MII oocyte and blastocyst rates. In contrast, oocytes denuded of cumulus cells were not able to take full advantage of the glucose in the medium, as only pyruvate was able to increase the MII rate and the subsequent early embryo developmental ability. Treatment of cumulus enclosed oocytes undergoing maturation with 200 μM dehydroepiandrosterone (DHEA), a pentose phosphate pathway inhibitor, or 2 μM iodoacetate (IA), a glycolysis inhibitor, significantly reduced GHS, intra-oocyte ATP, maternal gene expression, and MPF activity levels. DHEA was also able to increase ROS and reduce the levels of NADPH. Moreover, blastocysts of the DHEA- or IA-treated groups presented higher apoptosis rates and markedly lower cell proliferation cell rates than those of the non-treated group. In conclusion, our results suggest that oocytes maturing in the presence of 10% PFF can make full use of energy sources through glucose metabolism only when they are accompanied by cumulus cells, and that pentose phosphate pathway (PPP) and glycolysis promote porcine oocyte cytoplasmic maturation by supplying energy, regulating maternal gene expression, and controlling MPF activity.

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Section: Discussionsupporting

confidence: 91%

The Role of Glucose Metabolism on Porcine Oocyte Cytoplasmic Maturation and Its Possible Mechanisms

et al. 2016

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“…In the present study we did not find a relationship between E 2 concentration and the resumption of meiosis in oocytes. This observation is in agreement with the findings of in vitro experiments [ 36 ], where it was suggested that E 2 is not involved in nuclear maturation of pig oocytes but rather may promote changes in calcium activity during oocyte cytoplasmic maturation [ 37 ].…”

Section: Discussionsupporting

confidence: 92%

The effect of hormonal estrus induction on maternal effect and apoptosis-related genes expression in porcine cumulus-oocyte complexes

Bogacki

Wasielak

Kitewska

et al. 2014

Reprod Biol Endocrinol

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BackgroundThe effect of hormonal estrus induction on maternal effect (MATER - maternal antigen that embryo requires, ZAR-1 - zygote arrest 1, and BMP15 - bone morphogenetic protein 15) and apoptosis-related genes expression (BCL-2 and BAX) in porcine cumulus-oocyte complexes (COCs) and selected follicular parameters was investigated in this study.MethodsGilts were divided into three groups: (I) with natural estrus; (II) stimulated with PMSG/hCG; and (III) with PMSG/hCG + PGF2alpha. Analysis of maternal effect and apoptosis-related transcripts expression in COCs, and progesterone synthesis pathway genes expression (P450scc and 3betaHSD) in granulosa cells was performed by qPCR. BMP15 protein expression in follicular fluid (FF) was analyzed by western blot. Oocyte nuclear maturation was assessed by aceto-orcein staining. Progesterone (P4) and estradiol (E2) concentrations in FF and serum were measured by ELISA. Data were analyzed with the one-way ANOVA and Bonferroni post-test or Kruskal-Wallis test and Dunns post-test.ResultsThe highest expression of MATER, ZAR-1, and BMP15 genes was found in COCs recovered from gilts treated with PMSG/hCG when compared to PMSG/hCG + PGF2alpha-stimulated or non-stimulated gilts. Hormonal treatment did not affect the BMP15 protein expression in FF, but increased the expression of genes participating in P4 synthesis in granulosa cells. The higher percentage of immature oocytes was found in PMSG/hCG-treated when compared to the non-stimulated gilts. The expression of BCL-2 and BAX mRNA, and BCL-2/BAX mRNA ratio was significantly higher in COCs derived from PMSG/hCG-treated when compared to PMSG/hCG + PGF2alpha-treated or non-stimulated subjects. The level of P4 in serum was similar in animals from all experimental groups, while its concentration in FF was greater in gilts subjected to PMSG/hCG treatment than in PMSG/hCG + PGF2alpha-stimulated and non-stimulated gilts. The concentration of E2 did not differ in the serum or FF between the control group and the hormonally stimulated groups.ConclusionsHormonal induction of estrus affected maternal effect gene transcripts levels in COCs and and oocyte nuclear maturation. The inclusion of PGF2alpha into the stimulation protocol enabled maintaining of physiological concentration of P4 in FF. Additionally, both hormonal treatments seem to be beneficial for apoptosis prevention through increasing BCL-2/BAX transcript ratio.

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“…Next step of the work we determined the concentrations of 17-estradiol and progesterone in culture media conditioned with maturing OCCs, depending on the culture system used, considering the importance of steroid hormones for nuclear and cytoplasmic maturation of bovine cattle oocytes [25]. At the end of the culture, the content of 17-estradiol in the two-phase system without hormones (448±25 pg/ml) was less than in the single-phase system (583±27 pg/ml) 1.3 times (p < 0.01) (Fig.…”

Section: Development Sex Steroid Hormonesmentioning

confidence: 99%

Bovine Oocyte Ability to Embryonic Development When Maturing in Different Two-Phase Culture Systems

Сингина¹

2017

S-h. biol.

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Studies of the role of steroid hormone in the regulation of oocyte maturation in cattle

Abstract: Background: The objective of this study was to investigate whether the steroid hormone(s) secreted from cumulus-oocyte complexes (COCs) is a prerequisite for bovine oocyte maturation and cumulus expansion using aminoglutethimide (AGT), a P450 cholesterol side-chain cleavage inhibitor.

Cited by 40 publications

References 38 publications

The Role of Glucose Metabolism on Porcine Oocyte Cytoplasmic Maturation and Its Possible Mechanisms

The Role of Glucose Metabolism on Porcine Oocyte Cytoplasmic Maturation and Its Possible Mechanisms

The effect of hormonal estrus induction on maternal effect and apoptosis-related genes expression in porcine cumulus-oocyte complexes

Bovine Oocyte Ability to Embryonic Development When Maturing in Different Two-Phase Culture Systems

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