Studies of Ultraviolet Irradiation Phenomena -- An Approach to the Problems of Bacteriophage Reproduction

Bowen, G.

doi:10.1101/sqb.1953.018.01.035

Cited by 14 publications

(2 citation statements)

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“…The Effect of Ultraviolet Light on the Isolated Bacteriophages UV sensitivity was determined by exposing phage lysate to a UV lamp [37], and the survival of the phages was determined by the plaque assay technique [36].…”

Section: Physical Characterizationmentioning

confidence: 99%

Production of Dothiepin sulfoxide Carbonyl compounds by the phage resistant Streptomyces coeruleorubidus.

Magdy

2023

Preprint

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Researchers are increasingly looking for novel antibiotics due to the lack of new methicillin-resistant antibiotics against staphylococcus aureus (MRSA) and anti-Vancomycin-resistant Enterococci (VRE) matter. Streptomyces is the source of 66% of used antimicrobial substances. Moreover, improving phage-resistant strains of Streptomyces disposed of the phages and significantly increased the production of antimicrobial substances. In this study, 58 isolates of Actinobacteria were isolated from seven soil samples of rhizospheric soil in Egypt and tested for antimicrobial activity. A Streptomyces coeruleorubidus strain showed a strong antimicrobial effect on five groups of Gram +ve and Gram –ve microorganisms are known to be potential human pathogens. Colonies of S. coeruleorubidus were selected and identified following the International Streptomyces Project (ISP) method and the 16S rRNA sequence appeared to be 96.47–100% similar to various Streptomyces spp. The metabolite produced was identified as Dothiepin sulfoxide with advanced spectroscopy Nuclear magnetic resonance (NMR) spectroscopy, and gas chromatography-mass spectrometry instrument(GC mass). Unfortunately, a lysis of the production strain was observed. Three Streptomyces phages (ϕPRSC1, ϕPRSC2, and ϕPRSC4) were isolated and characterized using S. coeruleorubidus as a bacteria indicator. DNA of phage was digested with two restriction enzymes, EcoRI and HindIII. DNA of the phage ϕPRSC2 is sensitive to HindIII and fragments into two different banding patterns, whereas DNA of ϕPRSC1 and ϕPRSC4 is resistant to both enzymes. The phage-resistant bacterial strain was more stable than the original sensitive strain. This study suggested using phage-resistant strains of S. coeruleorubidus to produce the antimicrobial metabolite Thiocarbamic acid, N,N-dimethyl, -1,3-diphenyl-2-butenyl ester (Dothiepin sulfoxide). While screening for new antibiotics produced by actinomycetes, colonies that do not appear lysed must be selected.

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Section: Physical Characterizationmentioning

confidence: 99%

Production of Dothiepin sulfoxide Carbonyl compounds by the phage resistant Streptomyces coeruleorubidus.

Magdy

2023

Preprint

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“…Photo-reactivation of phage T2r DNA within infected cells was shown to consist of distinct light reaction and dark reaction steps, each of these steps following first-order kinetics (23,24). These conclusions were reached in experiments in which a series of short high-intensity light flashes were used.…”

Section: Photo-reactivationmentioning

confidence: 99%

Deoxyribonucleic acid repair in bacteriophage.

Bernstein¹

1981

Microbiological Reviews

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These numbers of lethal lesions were calculated from values given in reference 6 as 0.1% survival dose ratios. dThese data are from V. Johns (personal communication). 'These values were calculated for the purpose of this table from curves presented in the references. f Mutants of gene 58-61 were originally misclasefied into two complementation groups, corresponding to genes 58 and 61. Subsequently, however, it was recognized (219) that these mutations belong to one cistron, and the gene was then designated

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Génétique des Bactériophages

Fredericq

1958

Handbuch Der Virusforschung

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Studies of Ultraviolet Irradiation Phenomena -- An Approach to the Problems of Bacteriophage Reproduction

Cited by 14 publications

References 0 publications

Production of Dothiepin sulfoxide Carbonyl compounds by the phage resistant Streptomyces coeruleorubidus.

Production of Dothiepin sulfoxide Carbonyl compounds by the phage resistant Streptomyces coeruleorubidus.

Deoxyribonucleic acid repair in bacteriophage.

Génétique des Bactériophages

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