Studies of Urinary 17-Ketosteroid Excretion by Means of a New Micro-Chromatographic Fractionation Procedure*

Zygmuntowicz, A. S.; Wood, Margery; Christo, Eliane da Silva; Talbot, Ńathan B.

doi:10.1210/jcem-11-6-578

Cited by 49 publications

(6 citation statements)

References 17 publications

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“…Rotations were measured in CHCl8 (at 15-20') and ultraviolet absorption spectra in ethanol. Analysis was by Weiler and Zygmuntowicz, Wood, Christo & Talbot (1951) and colorimetric readings were taken at 430 and 520 m,u. and corrected (Medical Research Council, 1951).…”

Section: I956mentioning

confidence: 99%

Indirect analysis of corticosteroids. 3. The determination of steroidal dihydroxyacetones

Norymberski¹,

Stubbs²

1956

Biochemical Journal

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Section: I956mentioning

confidence: 99%

Indirect analysis of corticosteroids. 3. The determination of steroidal dihydroxyacetones

Norymberski¹,

Stubbs²

1956

Biochemical Journal

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“…Starting with microfractionation systems, the collected fractions often were found to be in the milliliter range. 30−33 We found that this naming system was used already in publications dated as early as 1951. 30 Successively, platforms working in the microliter range were called nanofractionation.…”

mentioning

confidence: 99%

“…30−33 We found that this naming system was used already in publications dated as early as 1951. 30 Successively, platforms working in the microliter range were called nanofractionation. 34−36 Therefore, in this study we called the presented nanoliter range fractionation platform picofractionation.…”

mentioning

confidence: 99%

“…The term “picofractionation” was chosen to continue the already existing nomenclature for fractionation platforms. Starting with microfractionation systems, the collected fractions often were found to be in the milliliter range. − We found that this naming system was used already in publications dated as early as 1951 . Successively, platforms working in the microliter range were called nanofractionation. − Therefore, in this study we called the presented nanoliter range fractionation platform picofractionation.…”

mentioning

confidence: 99%

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Bioactivity Profiling of Small-Volume Samples by Nano Liquid Chromatography Coupled to Microarray Bioassaying Using High-Resolution Fractionation

et al. 2019

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High-throughput screening platforms for the identification of bioactive compounds in mixtures have become important tools in the drug discovery process. Miniaturization of such screening systems may overcome problems associated with small sample volumes and enhance throughput and sensitivity. Here we present a new screening platform, coined picofractionation analytics, which encompasses microarray bioassays and mass spectrometry (MS) of components from minute amounts of samples after their nano liquid chromatographic (nanoLC) separation. Herein, nanoLC was coupled to a low-volume liquid dispenser equipped with pressure-fed solenoid valves, enabling 50-nL volumes of column effluent (300 nL/min) to be discretely deposited on a glass slide. The resulting fractions were dried and subsequently bioassayed by sequential printing of nL-volumes of reagents on top of the spots. Unwanted evaporation of bioassay liquids was circumvented by employing mineral oil droplets. A fluorescence microscope was used for assay readout in kinetic mode. Bioassay data were correlated to MS data obtained using the same nanoLC conditions in order to assign bioactives. The platform provides the possibility of freely choosing a wide diversity of bioassay formats, including those requiring long incubation times. The new method was compared to a standard bioassay approach, and its applicability was demonstrated by screening plasmin inhibitors and fibrinolytic bioactives from mixtures of standards and snake venoms, revealing active peptides and coagulopathic proteases.

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“…of the 17-ketosteroids was then performed, according to Callow's modification (1938) of Zimmermanns method (1935). Chromatography of the extract was carried out according to the micromethod of Zygmuntowicz et al (1951). To allow of iden¬ tification with infrared spectrography, however, a modified method has been developed.…”

mentioning

confidence: 99%

A Steroid Metabolic Study on Adrenalectomized Patients After Administration of Compounds E and F and Reichstein's Substance S

Plantin

1954

Acta Endocrinologica

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When steroid metabolic investigations are performed on patients with intact adrenals, the question always arises, how do administered steroids affect the secretion of the adrenals themselves? Hence, patients who have undergone total adrenalectomy are suitable subjects for the study of this problem. Despite the report of Laidlaw et al. (1952) that continued steroid excretion takes place after adrenalectomy, there is reason to believe that in adrenalectomized women and orchidectomized, adrenalectomized men without substitution therapy, the steroid excretion is of an order that cannot fundamentally affect the results in steroid metabolic studies. In some cases we have been able to demonstrate that as early as 48 hours after discontinuation of substitution therapy the 17\ x=req-\ ketosteroid excretion is very small in these patients (Birke & Plantin, 1953). When it was reported by Cohen (1951) that the corticoids of the urine are conjugated to glucuronic acid and that a substantially greater yield is obtained with \g=b\-glucuronidasehydrolysis than with acid hydrolysis, a study of the effect of administered steroids on corticoid and 17-ketosteroid excretion seemed in¬ dicated, to give an idea of the quantity converted to the respective metabolites. PRESENT INVESTIGATION Method Determination of the 17-ketosteroids: The urine was hydrolized by boiling with sulphuric acid. A continuous separate extraction was then performed with ethyl ether. The crude extract was separated with Girard's reagent T. Colorimetrie determination

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Studies of Urinary 17-Ketosteroid Excretion by Means of a New Micro-Chromatographic Fractionation Procedure*

Cited by 49 publications

References 17 publications

Indirect analysis of corticosteroids. 3. The determination of steroidal dihydroxyacetones

Indirect analysis of corticosteroids. 3. The determination of steroidal dihydroxyacetones

Bioactivity Profiling of Small-Volume Samples by Nano Liquid Chromatography Coupled to Microarray Bioassaying Using High-Resolution Fractionation

A Steroid Metabolic Study on Adrenalectomized Patients After Administration of Compounds E and F and Reichstein's Substance S

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