Studies on 3-indoleacetic acid metabolism. VII. Metabolism of radioactive 3-indoleacetic acid by pea roots

Andreae, W. A.; Robinson, J. R.; Ysselstein, M. W. H. Van

doi:10.1104/pp.36.6.783

Cited by 18 publications

(9 citation statements)

References 10 publications

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“…Further, metabolism of NAA in both detached and intact leaves were similar to that occurring in root tips, stem segments, and leaf discs (see citations in the Introduction). Similar to other studies (4,6,39,40) an initial lag period of 2 to 4 hr occurred before the aspartic acid conjugation system was induced in cowpea leaves (Fig. 3A), and the formation of NAGlu was immediate following uptake of NAA (Fig.…”

Section: Discussionsupporting

confidence: 68%

Mechanism of Naphthaleneacetic Acid Conjugation

Gören¹,

Bukovac²

1973

Plant Physiol.

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Formation of naphthaleneacetic acid-glucose (NAGlu) in detached leaves, floating on a-naphthaleneacetic acid-1-V4C (NAA, 0.05 microcurie per milliliter, 3.1 ,uM)-buffer solution (phosphate-citrate, pH 4.2) began immediately while there was a 2-to 4-hour lag before NAA-asparatate (NAAsp) could be detected. Subsequent increase in the NAAsp conjugate reflected a decrease in free NAA to 1 to 2 % of the total radioactivity taken up. Pretreatment with 31 gM 'C-NAA for 18 hours doubled NAAsp formation after transfer for 4 hours to '4C-NAA. Pretreatment with ethylene, as ethephon (up to 400 milligrams per liter) or ethylene gas (10 microliters per liter), did not induce NAAsp formation. In the presence of NAA, ethylene had no effect on NAA conjugation. Similarly, CO2 (5%) did not modify the formation of the conjugates. Rhizobitoxine (1.87 uM) inhibited NAA-induced ethylene production but did not prevent NAA-induced formation of NAAsp. We concluded that the conjugation of NAA with aspartic acid is not mediated by ethylene.

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Section: Discussionsupporting

confidence: 68%

Mechanism of Naphthaleneacetic Acid Conjugation

Gören¹,

Bukovac²

1973

Plant Physiol.

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“…But a 1 % indoleacetic acid paste, substituted for the younger to older leaf-blades (No. [1][2][3][4][5][6][7][8], slows abscission only as miluch as the normal intact blades do (29). One would expect that since all these -treated petioles are presumably getting as much auxin ats petiole numTher one, that !they all should stay on as long as numlber one.…”

mentioning

confidence: 99%

Transport and Metabolism of Indole-3-Acetic Acid in Coleus Petiole Segments of Increasing Age

Veen

Jacobs

1969

Plant Physiol.

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“…Analysis of the tissue for radioactive components was achieved by a modification of the procedure of Andreae et al (2). The tissue was removed by filtration through 4 layers of cheesecloth and then immediately rinsed in running tap water for approximately 1 minute followed by several rinses in distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…The remaining bathing solution was extracted by the procedures of Andreae et al (2) for analysis by paper chromatography. The solution was saturated with ammonium sulfate, acidified to pH 2.5, and extracted 3 times with 1-butanol.…”

Section: Methodsmentioning

confidence: 99%