Studies on a Blood Serum Proteolytic Enzyme with Particular Reference to Gastric Secretory Function

Chinn, Austin B.

doi:10.1016/s0016-5085(19)36280-8

Cited by 24 publications

(8 citation statements)

References 6 publications

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“…We also confirm the results of Mirsky et al (1952) and of Hoar and Browning (1956) who found that the mean value for normal women was not very different from that of normal men and that the level dropped only slightly with increasing age. In keeping with other reports (Chinn, 1953;Spiro, Ryan, and Jones, 1956;Sievers and Fischer, 1957;Nolan, 1958), our patients with pernicious anaemia had low serum pepsinogen levels. However, our results are at variance with some of the observations of Spiro and Schwartz (1958) and Nolan (1958).…”

Section: Discussionsupporting

confidence: 93%

The serum pepsinogen level with special reference to the histology of the gastric mucosa

Böck¹,

Arapakis²,

Witts³

et al. 1963

Gut

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Section: Discussionsupporting

confidence: 93%

The serum pepsinogen level with special reference to the histology of the gastric mucosa

Böck¹,

Arapakis²,

Witts³

et al. 1963

Gut

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“…The highly significant reduction in the values from patients with pernicious anaemia compared with those from control subjects confirms previous findings. Nevertheless there is a considerable overlap between the results in these two groups of subjects, in agreement with the results of Chinn (1953), Hoar and Browning (1956), and Mirsky et al (1952).…”

Section: Discussionsupporting

confidence: 90%

“…Patients with pernicious anaemia and related conditions show lower levels of serum pepsinogen than normal subjects (Chinn, 1953;Hoar and Browning, 1956;Mirsky, Futterman, and Kaplan, 1952;Nolan, 1958;Singh and Shinton, 1965). Bock, Arapakis, Witts, and Richards (1963), using a modification of the method of Edwards, Jepson, and Wood (1960) with freeze-dried human plasma as the substrate, found a correlation between the serum pepsinogen level and the histological appearance of gastric biopsy specimens.…”

mentioning

confidence: 99%

Estimation of serum pepsinogen.

Ilic¹,

Spray²

1966

Gut

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EDITORIAL SYNOPSIS A modified method for estimating serum pepsinogen using haemoglobin as substrate offers some advantages over those previously published.Measurement of serum pepsinogen, the proteolytic activity of serum at about pH 2-0, has been used as one of a number of indirect tests of gastric function. Patients with pernicious anaemia and related conditions show lower levels of serum pepsinogen than normal subjects (Chinn, 1953;Hoar and Browning, 1956;Mirsky, Futterman, and Kaplan, 1952;Nolan, 1958;Singh and Shinton, 1965). Bock, Arapakis, Witts, and Richards (1963), using a modification of the method of Edwards, Jepson, and Wood (1960) with freeze-dried human plasma as the substrate, found a correlation between the serum pepsinogen level and the histological appearance of gastric biopsy specimens.As part of an investigation being made in this Department of the incidence of 'latent pernicious anaemia ' (Callender and Spray, 1962) among the relatives of patients with pernicious anaemia, we wished to measure pepsinogen in the serum of a large number of subjects. A study of the method used by Bock et al. (1963) showed it to be both too laborious and unsuitable in other respects for use in a survey requiring several hundred estimations. A modified method has therefore been developed, using haemoglobin as substrate, and incorporating a buffer system to control the pH of the mixtures of substrate and serum. Both substrate and serum are treated with charcoal to reduce the blank readings, and the use of the Folin-Ciocalteau reagent to measure tyrosine has been eliminated. This method appears to offer some advantages over those previously published, and it is described in detail in this paper, together with the results obtained in normal subjects and patients with pernicious anaemia. METHODSsuBsTRATE Haemoglobin type II powder (Sigma London Chemical Co., Ltd.) was used throughout as the substrate. Haemoglobin (2 g.) was dissolved in 25 ml. water containing 3-4 drops of 0-5 N NaOH solution, using a magnetic stirrer. The volume was made up to 100 ml. with sodium acetate-HC1 buffer (16-4 g. anhydrous sodium acetate +260 ml. N HCI per litre). The solution was treated with 1 g. charcoal (Norit NK, Hopkin and Williams Ltd.) for 15 minutes, and the charcoal was removed by centrifuging for 20 minutes at 2,400 r.p.m. SERUM Blood was obtained by venepuncture and was allowed to clot in glass vessels. The clots were broken up with glass rods, the samples were centrifuged, and the serum was stored at -15 'C until required for estimation. After thawing at room temperature and mixing, serum (3 ml.) was treated with about 5 mg. charcoal for 15 minutes and the charcoal was removed by centrifugation.

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“…There is a virtual absence of pepsinogen activity in plasma and urine following total gastrectomy and in cases of pernicious anaemia (Chinn, 1953;Hirschowitz, 1955). Conversely, gastric pepsin secretion and plasma pepsinogen activity are both significantly higher in patients with duodenal ulcer than in normal persons (Chinn, 1953;Van Goidsenhoven, Wilkoff, and Kirsner, 1958). In addition, significant correlations have been shown between gastric pepsin and plasma pepsinogen activity in normal people by Janowitz, Levy, and Hollander, (1950) and Van Goidsenhoven et al (1958).…”

Section: Resultsmentioning

confidence: 99%

“…The exact relation between peptic cell activity and plasma pepsinogen activity is uncertain. There is a virtual absence of pepsinogen activity in plasma and urine following total gastrectomy and in cases of pernicious anaemia (Chinn, 1953;Hirschowitz, 1955). Conversely, gastric pepsin secretion and plasma pepsinogen activity are both significantly higher in patients with duodenal ulcer than in normal persons (Chinn, 1953;Van Goidsenhoven, Wilkoff, and Kirsner, 1958).…”

Section: Resultsmentioning

confidence: 99%

The effect of phenylbutazone (Butazolidin) on plasma pepsinogen activity

Muirden¹

1961

Gut

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Studies on a Blood Serum Proteolytic Enzyme with Particular Reference to Gastric Secretory Function

Cited by 24 publications

References 6 publications

The serum pepsinogen level with special reference to the histology of the gastric mucosa

The serum pepsinogen level with special reference to the histology of the gastric mucosa

Estimation of serum pepsinogen.

The effect of phenylbutazone (Butazolidin) on plasma pepsinogen activity

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