Studies on Actinomycetales producing antibiotics only on agar culture. I. Screening, taxonomy and morphology-productivity relationship of Streptomyces halstedii, strain SF-1993.

Shomura, Takashi; Yoshida, Junko; Amano, Shoichi; Kojima, Michio; Inouye, Shigeharu; Niida, Taro

doi:10.7164/antibiotics.32.427

Cited by 72 publications

(34 citation statements)

References 5 publications

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“…Preliminary screenings for antibiotic production were carried out (Shomurat et al 1979). The Streptomyces strains were spot inoculated in Petri plates containing glycerol asparagine medium for 7 days.…”

Section: Spot Inoculation Methodsmentioning

confidence: 99%

Bioactive potential of Streptomyces associated with marine sponges

Dharmaraj

Sumantha

2009

World J Microbiol Biotechnol

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The present work deals with isolation of Streptomyces associated with marine sponges and its bioactive potential. Streptomyces sp. were isolated from the marine sponges Callyspongia diffusa, Mycale mytilorum, Tedania anhelans and Dysidea fragilis. From the initial screening, 94 cultures of Streptomyces were obtained and from these 58 cultures exhibited antagonism against bacteria, 36 strains against fungi and 27 strains exhibited broad spectrum activity against both. The submerged culture extracts of the 58 anti-bacterial isolates were analysed and of these 58 strains, 37 strains showed positive inhibition against Bacillus subtilis, 43 against Staphylococcus aureus, 10 against Vibrio cholerae and 10 against Escherichia coli. The antifungal activities of the 36 strains were also evaluated and 27 strains showed positive inhibition against Aspergillus niger, 23 against Saccharomyces cerevisiae and 16 against Candida albicans. The production of polyene substances from the active extracts was confirmed by UV spectral analysis by the absorbance peaks that ranged from 225 to 262 nm and the TLC (R f values) ranging from 0.40 to 0.78. The results indicate that Streptomyces strains isolated from marine sponges produce potential antibacterial, antifungal and broad spectrum antibiotic compounds.

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Section: Spot Inoculation Methodsmentioning

confidence: 99%

Bioactive potential of Streptomyces associated with marine sponges

Dharmaraj

Sumantha

2009

World J Microbiol Biotechnol

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“…For all selected isolates, the antibacterial activity was more significant in solid than in liquid media. It has been established that solid medium is more adequate to the development of the isolates and the production of antibiotics (Iwai and Omura 1982;Shomura et al 1979;Badji et al 2005). After extraction by the ethyl acetate and butanol, 17 organic and 4 aqueous extracts (ST 3 , R 5 , T 5 and AS 8 ) show the antibacterial activity against M. luteus.…”

Section: Characterizations Of Active Isolatesmentioning

confidence: 99%

Actinomycetes from Moroccan habitats: isolation and screening for cytotoxic activities

Anibou

Chait²,

Zyad

et al. 2008

World J Microbiol Biotechnol

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In our screening for actinomycetes showing cytotoxic activities, 8 samples were collected from various Moroccan habitats, 136 isolates were tested for their capacity to produce antibacterial compounds against gram positive bacteria. Thirty-seven strains of these isolates were active against Gram-positive bacteria. Using the following steps of primary screening: antibacterial activity, confrontation between the isolates and toxicity to Artemia salina; fifteen different isolates were used for further investigation. The aqueous extracts of Streptomyces sp. T5 and Streptomyces sp. AS8 were selected for their cytotoxic activity against Hep2, BSR and P815 cell lines, and two active compounds were observed on HPLC. The two isolates exhibited high activity against human cancer cell lines and were inactive on PBMC cell lines. Furthermore, the Streptomyces sp. T5 extract showed a proliferative activity.

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“…The test strains were seeded in cross streaks to actinomycete culture. The antibacterial inhibition was evaluated after incubation at 30°C for 24 to 48 h. The results were confirmed using the agar piece method (Petrosyan et al, 2003;Saadoun and Al moumani, 1997;Shomura et al, 1979). The actinomycetes isolates were grown in scratches tightened in SCA medium and incubated at 28°C for 7 to 10 days.…”

Section: Detection Of Antimicrobial Activitymentioning

confidence: 98%

“…It is worth nothing that antibacterial activity was found in crude extract of actinomycetes isolates, performed from both solid and liquid culture. Indeed, it is recognized that antibiotic production by actinomycetes is more effective from solid than liquid medium and many strains are unable to produce antibiotics from liquid medium (Iwai and Omura, 1982;Shomura et al, 1979).…”

Section: Partial Characterization Of the Antimicrobial Productsmentioning

confidence: 99%