Studies on Adenosine Triphosphate Transphosphorylases

Transcortin. A Corticosteroid-Binding Protein of Plasma. IX. Isolation and Characterization*

“…The nitrogen content of the protein was not determined. The was calculated on the best available dry weight3 of protein as recommended by other authors (Mahowald et al, 1962;Spero et al, 1965). The sum of these values was then divided into 100 to obtain a correction factor for the analysis values.…”

Molecular weight and amino acid composition of five-times-crystallized phosphoglucose isomerase from rabbit skeletal muscle

“…In addition, similar alkylation experiments were conducted in 1 % sodium dodecyl sulfate; however, difficulty was experienced in achieving reproducible extents of carboxymethylation. d The value in parentheses is calculated by assuming a recovery of 80% (Gundlach et al, 1959;Noltmann et al, 1962). 6 Compare Chatterjee and Noltmann (1967b).…”

“…Travel of the boundary is from right to left. et al (1958) on Beckman-Spinco automatic amino acid analyzers Models 120B and 120C with attention to experimental details elaborated upon in the past Noltmann et al, 1962;Moore and Stein, 1963;Tristram and Smith, 1963).…”

“…Determination of Cysteine or Half-cystine. The following methods, each based on a different analytical principle, were employed: conversion into cysteic acid by oxidation with performic acid (Moore, 1963); conversion into S-carboxymethylcysteine by alkylation with iodoacetic acid after denaturation and reduction (Cole et al, 1958;Noltmann et al, 1962); air oxidation at pH 6.8 after acid hydrolysis (Hirs et al, 1954); titration with p-mercuri benzoate (Boyer, 1954); and amperometric titration of sulfhydryl groups as the Tris-silver complexes (Benesch et al, 1955). Determination of Tryptophan.…”

“…Chromatographic determination by ion-exchange chromatography after alkaline hydrolysis with barium hydroxide. Two methods of hydrolysis were compared: (a) the procedure of Ray and Koshland (1962); (b) a modification1 of an earlier method (Noltmann et al, 1962), consisting of hydrol-…”