Transcortin. A Corticosteroid-Binding Protein of Plasma. IX. Isolation and Characterization^*

Abstract

Plasma from male subjects with cancer of the prostate, who were receiving diethylstilbestrol treatment, was fractionated successively on columns of diethylaminoethylcellulose, Sephadex G-25, hydroxylapatite, and Sephadex G-75. Transcortin was detected

Transcortin. Comparison of the cortisol-binding globulin from human and cavian plasma

“…All chromatographic steps were performed at 6°. DEAE-cellulose chromatography and subsequent desalting on Sephadex G-25 were performed as previously described (Slaunwhite et al, 1966). The resin was equilibrated with starting buffer which was 0.025 m in NaCl and 0.015 m in NaH2P04 at pH 5.0.…”

“…The molecular weight of human transcortin prepared in the present study was determined as previously described (Slaunwhite et al, 1966).…”

“…The preparation of a highly purified CBG, which has been named transcortin (Slaunwhite and Sandberg, 1959), from the plasma of normal and estrogen-treated human subjects was first reported by Seal and Doe (Seal and Doe, 1962a). Preliminary efforts by Slaunwhite (Slaunwhite et al, 1966) provided information regarding the molecular weight and chemical composition of transcortin isolated from plasma of male subjects treated with diethylstilbestrol.…”

Genetic Studies of Low-Abundance Human Plasma Proteins

“…CBG was first isolated from human plasma by Seal and Doe [14] and later by Slaunwhite et al [15] and Muldoon and Westphal [10], The reported molecular weights range from 49,000 to 58,000 daltons [1,6,8,10,15]. Approximately 30% of this weight is carbohydrate, with varia tion in glycosylation partly responsible for the apparent molecular weight heter ogeneity [2,10,17].…”

The Effect of Cortisone on the Volume and Total Protein Content of Mouse Liver Nuclei

“…These results suggest that some component which is present in the in vivo system is absent from the in vitro system . That this component may be a plasma protein is suggested by the facts that : a) cortisone binds selectively to a human plasma protein (8,11), and b) the perfusion of whole rat liver with medium containing cortisone produces little induction of gluconeogenic enzymes, whereas the addition of whole rat blood to such a perfusion system produces a marked induction (1) . It should also be noted that shortly after in vivo administration the hormone is found bound to rat liver histone (12) .…”