Studies on Agranulocytosis. III. The Reduced Glutathione (GSH) Content of Leukocytes of Normals and Patients Recovered from Agranulocytosis

Pisciotta, Anthony V.; Daly, Mary B.

doi:10.1182/blood.v16.5.1572.1572

Cited by 8 publications

(1 citation statement)

References 5 publications

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“…Such a reaction might thus contribute to defervescence. Although there is at present no evidence that the proteolytic mechanism actually operates under the conditions which pertain in inflammatory exudates, it is of interest that polymorphonuclear leucocytes contain detectable amounts of reduced glutathione (27,28).…”

Section: Discussionmentioning

confidence: 99%

Studies on the Pathogenesis of Fever

Kaiser

Wood

1962

The Journal of Experimental Medicine

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Polymorphonuclear leucocytes obtained from acute peritoneal exudates in rabbits are capable, when appropriately stimulated, of producing large amounts of endogenous pyrogen (1-3). There is mounting evidence that this pyrogenic factor plays an important role in the pathogenesis of fever (4, 5). Exploratory studies relating to the production of leucocytic pyrogen in vitro have revealed t h a t the process is temperature-dependent and that it results in a substantial net increase of active pyrogen per cell during incubation in saline (2). Since these findings are in keeping with the hypothesis that the active pyrogen is formed, either from an inactive precursor or b y de novo synthesis within the cell, experiments were undertaken to investigate the possible effects of certain enzyme inhibitors upon the combined metabolic reactions involved in the formation and release of the pyrogen. MethodsThe technical procedures employed in obtaining appropriate quantifies of rabbit granulocytes from acute peritoneal exudates and in assaying the endogenous pyrogen which they produce during incubation in saline were identical with those of the preceding study (2). The standard aliquot of granulocytes used in aU experiments contained 3.5 × 108 polymorphonudear leucocytes from 12 to 18 hour peritoneal exudates. All cell suspensions or extracts to which enzyme inhibitors or activators had been added were brought to neutrality before being incubated, unless otherwise stated. After incubation, the samples to be assayed for pyrogenicity were dialyzed in ceUophane tubing against a minimum of 30 volumes of saline at 4°C for 20 hours. Three changes of saline were made during each dialysis. RESULTS Sulfhydryl Reagents.-Each of four standard aliquots of granulocytes harvested from 18 hour exudates was washed in 30 ml of cold normal saline x and was resuspended in 11.7 ml (3 X l0 T ceils per ml) of saline

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Section: Discussionmentioning

confidence: 99%