Studies on Bacillus subtilis Ribonuclease. III. Purification and Amino Acid Composition^*

Conformational stability and thermodynamics of folding of ribonucleases Sa, Sa2 and Sa3 1 1Edited by P. E. Wright

“…(1.57 mg À1 ml cm À1 , Sa2), 17,550 M À1 cm À1 (1.59 mg À1 ml cm À1 , Sa3) (Hebert et al, 1997), and 25,880 M À1 cm À1 (2.09 mg À1 ml cm À1 , Ba) (Lees & Hartley, 1966). Ultrapure urea was purchased from Ambion.…”

How to measure and predict the molar absorption coefficient of a protein

“…Entries 58-66 are from the Privalov lab (Privalov et al, 1989;Griko et al, 1994;Yu et al, 1994). For barnase and mutants, entry 67 is from Lees and Hartley (1966), and 68-72 are from Loewenthal et al (1991). For RNase T1 and mutants, entries 73-80, the t values are from Tables 1 and 7 .…”

A reversible thermal transition of the extracellular ribonuclease of Bacillus amyloliquefaciens

“…In large or small proteins, portions of the molecule may be stabilized by disulfide bridges, as would appear to be the case with pancreatic ribonuclease. The ribonuclease discussed in this paper is small (mol wt 10,700, *~94 residues) and contains no sulfur (Hartley et al, 1963; Lees and Hartley, 1966).…”

“…The enzyme was prepared from spent culture medium of strain H2. Simplification and improvement of the purification since our previous report (Lees and Hartley, 1966) is not relevant to this paper and will be reported elsewhere.1…”