Studies on Benzophenanthridine Alkaloid Production in Elicited Cell Cultures of Papaver somniferum L.

Tyler, Robert T.; Eilert, U.; Rijnders, C. O. M.; Roewer, I. A.; McNabb, C. K.; Kurz, Wolfgang

doi:10.1007/978-3-642-74551-5_22

Cited by 16 publications

(8 citation statements)

References 17 publications

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“…Brodelius et al (1989) conducted a series of experiments on the effects of a yeast elicitor on the benzophenanthridine alkaloids in cell cultures of Thalictrum rugosum and Escholtzia californica, and showed that the presence of the elicitor induced spectacular changes to the metabolism of these alkaloids. Tyler et al (1989), extending earlier studies by Eilert, Kurz & Constabel (1985) on the elicitation of Papaver somniferum cells with a Botrytis elicitor, have confirmed the observation that massive amounts of a potentially important alkaloid, sanguinarine, an antibiotic in oral hygiene products, are produced in response to this treatment and they have begun to examine the possibility of commerciai production. It is now clear that the fiux of metabolites in a number of secondary pathways can bt infiuenced by elicitor treatment, often via thf induction or amplification of various enzymes, thuf facilitating the molecular analysis of the pathway (Hahlbrock & Scheel, 1989;Bohlmann & Eilert 1994).…”

Section: {D) Elicitationsupporting

confidence: 58%

Manipulating secondary metabolism in cultured plant cells

Yeoman¹,

Yeoman

1996

New Phytologist

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summary This review attempts to present an overall strategy for the production of useful secondary metabolites by cultured plant cells. After consideration of the nature and utility of secondary metabolites and the possible role of these substances to the plant, the review focuses attention on the properties of the plant cells in culture and how the cell populations and their physical and chemical environment can be manipulated to encourage the synthesis and accumulation of secondary products. Finally, consideration is given to the involvement of genetic engineering in the production of cells to perform particular metabolic tasks and how these techniques might contribute to the development of a new strategy to enable the production of useful secondary metabolites on a commercial scale.

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Section: {D) Elicitationsupporting

confidence: 58%

Manipulating secondary metabolism in cultured plant cells

Yeoman¹,

Yeoman

1996

New Phytologist

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“…In certain cases, they enhance alkaloid production in several plant species, for example, berberine in Coptis japonica (Nakagawa et al 1986;Ikuta & Itokawa 1988) and sanguinarine in P. somniferum (Tyler et al 1989). However, in this study, it seems the plant growth regulators did not have effects on the accumulation of alkaloid, and the content of thebaine in tissues of P. bracteatum was identical with that of wild-type plants (Fig.…”

Section: Hplc Analysis Of Thebaine Alkaloidcontrasting

confidence: 52%

In vitro regeneration of Persian poppy (Papaver bracteatum)

2010

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Persian poppy (Papaver bracteatum Lindl.) is an important commercial source of medicinal opiates and related compounds. In this research, calli were induced from seeds, roots, cotyledons and hypocotyls of P. bracteatum at a high efficiency. The optimized callus induction media consisted of the Murashige and Skoog (MS) basic media supplemented with 1.0 mg/L 2, 4-dichlorophenoxyacetic acid (2,4-D), 0.1 mg/L kinetin and 15 mg/L ascorbic acid. The concentrations of 2,4-D and ascorbic acid were found critical to callus induction and proliferation. Subsequent subcultures resulted in excellent callus proliferation. Ascorbic acid at concentration 15 mg/L increased the callus proliferation significantly. Maximum callus growth was achieved when the explants were incubated at 25• C. MS salts at full strength were found inhibitory for callus induction, while ľ MS salts were found to favor callus induction. Shoot regeneration of calli in vitro was achieved on ľ MS medium containing 0.5 mg/L benzylamine purine and 1.0 mg/L naphthalene acetic acid. Analysis of alkaloid extracts from Persian poppy tissues by high-performance liquid chromatography showed that thebaine accumulated in the tissues of plants. The thebaine alkaloid profile of the Persian poppy is a well-defined model to evaluate the potential for metabolic engineering of thebaine production in P. bracteatum.

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“…Numerous research reports exist on the effects of plant growth regulators or elicitors on secondary metabolism of in vitro cultures, and in certain cases they enhance the isoquinoline alkaloid production in several plant species, for example, berberine in Coptis japonica (Nakagawa et al 1986;Ikuta and Itokawa 1989), sanguinarine in Eschscholtzia californica (Collinge and Brodelius 1989) and Papaver somniferum (Tyler et al 1989). However, most regulators used in this study showed no significant effects on alkaloid accumulation.…”

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confidence: 61%

Alkaloid production by somatic embryo cultures of Corydalis ambigua

Hiraoka

Bhatt

Sakurai

et al. 2004

Plant Biotechnology

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Corydalis ambigua Cham. et Schltdl. is a perennial herb of the family Papaveraceae and found in the northern areas of the Far East, including Japan. The plant has been used in traditional Chinese medicine or folk medicine as an analgesic, antirheumatic and emmenagogue (Hotta et al. 1989; Chou and Hsü 1992) and contains corybulbine, corydaline and cavidine (Fig. 1) as the major tetrahydroprotoberberine alkaloids in the tuber (Naruto and Kaneko 1973; Yasuda et al. 1988). These tertiary bases are converted to corresponding dehydrogenated quaternary bases, the protoberberine alkaloids (Figure 1), during the drying process of tubers (Yasuda et al. 1987). Dehydrocorydaline is thought to be one of the main effective constituents of the crude drug (Chou and Hsü 1992). Ito et al. (2001) proposed corydaline, dehydrocorydaline and corybulbine to be potential cancer chemopreventive agents. In addition, corydaline has been shown to have anti-thrombic action in vitro (Matsuda et al. 1988) and dehydrocorydaline is known for its antiallergic activity (Matsuda et al. 1997a), antiinflammatory activity (Kubo et al. 1994b; Matsuda et al. 1997b), inhibitory effects against aldose reductase (Kubo et al. 1994a) and insecticidal activity (Miyazawa et al.

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Studies on Benzophenanthridine Alkaloid Production in Elicited Cell Cultures of Papaver somniferum L.

Cited by 16 publications

References 17 publications

Manipulating secondary metabolism in cultured plant cells

Manipulating secondary metabolism in cultured plant cells

In vitro regeneration of Persian poppy (Papaver bracteatum)

Alkaloid production by somatic embryo cultures of Corydalis ambigua

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